Journal ArticleDOI
The determination of hydroxyproline in tissue and protein samples containing small proportions of this imino acid.
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TLDR
Method has been applied to a study of hydroxyproline distribution in cell particulates, tissue fluids, and purified plant and animal proteins, and significant amounts of hydroXYproline were found in crystalline preparations of pepsin, elastase.About:
This article is published in Archives of Biochemistry and Biophysics.The article was published on 1961-05-01. It has received 3756 citations till now. The article focuses on the topics: Hydroxyproline & Imino acid.read more
Citations
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Application of a PDGF-containing novel gel for cutaneous wound healing.
TL;DR: It is revealed that the monomeric nature of biological molecules in association with PDGF exerted simultaneous biomimetic and chemotactic effects and promoted wound healing.
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Development of reduced graphene oxide (rGO)-isabgol nanocomposite dressings for enhanced vascularization and accelerated wound healing in normal and diabetic rats.
Ponrasu Thangavel,Ramya Kannan,Balaji Ramachandran,Ganeshkumar Moorthy,Lonchin Suguna,Vignesh Muthuvijayan +5 more
TL;DR: Investigation showed that Isab’s+ rGO scaffold dressing could significantly accelerate the healing of normal and diabetic wounds and played a major role in shortening the inflammation phase and recruiting macrophages to enhance the early phase of wound healing.
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Dermal Collagen and Lipid Deposition Correlate with Tissue Swelling and Hydraulic Conductivity in Murine Primary Lymphedema
Joseph M. Rutkowski,Carl Erik Markhus,Christina C. Gyenge,Kari Alitalo,Helge Wiig,Melody A. Swartz +5 more
TL;DR: It is suggested that lymphedema associated with increased collagen and lipid accumulation counteracts an increased hydraulic conductivity associated with dermal swelling, which in turn further limits interstitial transport and swelling.
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“Milwaukee shoulder”—association of microspheroids containing hydroxyapatite crystals, active collagenase, and neutral protease with rotator cuff defects. iii. morphologic and biochemical studies of an excised synovium showing chondromatosis
TL;DR: Electronmicroscopy revealed calcific foci in microvilli which could easily escape into the adjacent joint space through areas denuded of synovial cells, and energy dispersive analysis showed elemental ratios consistent with hydroxyapatite.
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Quantitative study of sodium hypochlorite as an in vitro endodontic irrigant
TL;DR: Solutions of sodium hypochlorite were tested as root canal irrigants in single-rooted human teeth and the measure of effectiveness used was the hydroxyproline content of the irrigant, which reflects the amount of collagen-containing tissues dissolved.
References
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Journal ArticleDOI
The determination of hydroxyproline.
Robert E. Neuman,Milan A. Logan +1 more
TL;DR: A short and simple calorimetric method is here described that is applicable to the determination of hydroxyproline in hydrolysates of 40 to 100 y of collagen with a reproducibility of f2 per cent and an accuracy of f1 per cent as judged by recovery of hydroXYproline from elastin hydrolysate and from an amino acid mixture simulating collagen.
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[Microdetermination of hydroxyproline with chloramine-T and p-dimethylaminobenzaldehyde].
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Growth and sexual differentiation of hydra in mass culture
W. F. Loomis,H. M. Lenhoff +1 more
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Appendix—Notes on a modification of the Neuman & Logan method for the determination of the hydroxyproline
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Formation of collagen hydroxyproline in vitro
N. M. Green,D. A. Lowther +1 more
TL;DR: Stetten (1949) suggested that free hydroxyproline was not an intermediate in collagen biosynthesis and that hydroxylation of the proline residues occurred at the protein or peptide stage.