Journal ArticleDOI
The FET3 gene of S. cerevisiae encodes a multicopper oxidase required for ferrous iron uptake.
Candice C. Askwith,David J. Eide,Anthony Van Ho,Philip S. Bernard,Liangtao Li,Sandra Davis-Kaplan,David M. Sipe,Jerry Kaplan +7 more
TLDR
It is suggested that the catalytic activity of the Fet3 protein is required for cellular iron accumulation, similar to that of the blue multicopper oxidoreductases.About:
This article is published in Cell.The article was published on 1994-01-28. It has received 665 citations till now. The article focuses on the topics: Ferrous iron transport & Multicopper oxidase.read more
Citations
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Journal ArticleDOI
Multicopper Oxidases and Oxygenases
TL;DR: Copper sites have historically been divided into three classes based on their spectroscopic features, which reflect the geometric and electronic structure of the active site: type 1 or blue copper, type 2 (T2) or normal copper, and type 3 (T3) or coupled binuclear copper centers.
Journal ArticleDOI
Positional cloning of zebrafish ferroportin1 identifies a conserved vertebrate iron exporter
Adriana Donovan,Alison Brownlie,Alison Brownlie,Yi Zhou,Jennifer L. Shepard,Stephen J. Pratt,John Moynihan,Barry H. Paw,Anna Drejer,Bruce A. Barut,Bruce A. Barut,Agustín G. Zapata,Terence C. Law,Carlo Brugnara,Samuel E. Lux,Geraldine S. Pinkus,Jack L. Pinkus,Paul D. Kingsley,James Palis,Mark D. Fleming,Mark D. Fleming,Nancy C. Andrews,Nancy C. Andrews,Leonard I. Zon,Leonard I. Zon +24 more
TL;DR: The gene responsible for the hypochromic anaemia of the zebrafish mutant weissherbst is identified and Ferroportin1 function may be perturbed in mammalian disorders of iron deficiency or overload.
Journal ArticleDOI
A Novel Duodenal Iron-Regulated Transporter, IREG1, Implicated in the Basolateral Transfer of Iron to the Circulation
Andrew T. McKie,Paola Marciani,Andreas Rolfs,Karen Brennan,Kristina Wehr,Dalna Barrow,Silvia Miret,Adrian Bomford,Tim J Peters,Farzin Farzaneh,Matthias A. Hediger,Matthias W. Hentze,Robert J. Simpson +12 more
TL;DR: The isolation and characterization of a novel cDNA (Ireg1) encoding a duodenal protein that is localized to the basolateral membrane of polarized epithelial cells are described and it is concluded that IREG1 represents the long-sought duodental iron export protein and is upregulated in the iron overload disease, hereditary hemochromatosis.
Journal ArticleDOI
A novel iron-regulated metal transporter from plants identified by functional expression in yeast
TL;DR: In Arabidopsis, IRT1 is expressed in roots, is induced by iron deficiency, and has altered regulation in plant lines bearing mutations that affect the iron uptake system, providing the first molecular insight into iron transport by plants.
Journal ArticleDOI
Copper Active Sites in Biology
Edward I. Solomon,David E. Heppner,Esther M. Johnston,Jake W. Ginsbach,Jordi Cirera,Munzarin F. Qayyum,Matthew T. Kieber-Emmons,Christian H. Kjaergaard,Ryan G. Hadt,Li Tian +9 more
TL;DR: This review presents in depth discussions of all these classes of Cu enzymes and the correlations within and among these classes, as well as the present understanding of the enzymology, kinetics, geometric structures, electronic structures and the reaction mechanisms these have elucidated.
References
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Journal ArticleDOI
Basic Local Alignment Search Tool
TL;DR: A new approach to rapid sequence comparison, basic local alignment search tool (BLAST), directly approximates alignments that optimize a measure of local similarity, the maximal segment pair (MSP) score.
Journal ArticleDOI
Studies on transformation of Escherichia coli with plasmids
Douglas Hanahan,Douglas Hanahan +1 more
TL;DR: Competition with both transforming and non-transforming plasmids indicates that each cell is capable of taking up many DNA molecules, and that the establishment of a transformation event is neither helped nor hindered significantly by the presence of multiple plasmid molecules.
Journal ArticleDOI
Universal chemical assay for the detection and determination of siderophores
Bernhard Schwyn,J.B. Neilands +1 more
TL;DR: The universal method to detect and determine siderophores was developed by using their high affinity for iron(III) and was successfully used to screen mutants in the iron uptake system of two Rhizobium meliloti strains, DM5 and 1021.
Journal ArticleDOI
New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites.
R D Gietz,A Sugino +1 more
TL;DR: The production of new alleles of the LEU2, URA3 and TRP1 genes of Saccharomyces cerevisiae by in vitro mutagenesis is described and a unique series of yeast-Escherichia coli shuttle vectors derived from the plasmid pUC19 are constructed.