Journal ArticleDOI
The language of covalent histone modifications.
Brian D. Strahl,C D Allis +1 more
Reads0
Chats0
TLDR
It is proposed that distinct histone modifications, on one or more tails, act sequentially or in combination to form a ‘histone code’ that is, read by other proteins to bring about distinct downstream events.Abstract:
Histone proteins and the nucleosomes they form with DNA are the fundamental building blocks of eukaryotic chromatin. A diverse array of post-translational modifications that often occur on tail domains of these proteins has been well documented. Although the function of these highly conserved modifications has remained elusive, converging biochemical and genetic evidence suggests functions in several chromatin-based processes. We propose that distinct histone modifications, on one or more tails, act sequentially or in combination to form a 'histone code' that is, read by other proteins to bring about distinct downstream events.read more
Citations
More filters
Journal ArticleDOI
E2F-dependent histone acetylation and recruitment of the Tip60 acetyltransferase complex to chromatin in late G1.
Stefan Taubert,Chiara Gorrini,Scott R. Frank,Tiziana Parisi,Miriam Fuchs,Ho Man Chan,David M. Livingston,Bruno Amati +7 more
TL;DR: It is shown that activating E2F species are required for hyperacetylation of target chromatin in human cells and speculated that the activities of multiple HAT complexes account for E 2F-dependent acetylation, transcription, and S-phase entry.
Journal ArticleDOI
Involvement of Histone H3 Lysine 9 (H3K9) Methyltransferase G9a in the Maintenance of HIV-1 Latency and Its Reactivation by BIX01294 *
TL;DR: Evidence is presented that histone H3 Lys9 (H3K9) methyltransferase G9a is responsible for transcriptional repression of HIV-1 by promoting repressive dimethylation at H3K 9 and for the maintenance of viral latency.
Journal ArticleDOI
The MTE, a new core promoter element for transcription by RNA polymerase II
TL;DR: It is found that the loss of transcriptional activity upon mutation of a TATA-box or DPE can be compensated by the addition of an MTE, a novel downstream core promoter element that is important for transcription by RNA polymerase II.
Journal ArticleDOI
Human LSD2/KDM1b/AOF1 Regulates Gene Transcription by Modulating Intragenic H3K4me2 Methylation
Rui Fang,Andrew J. Barbera,Yufei Xu,Michael S. Rutenberg,Thiago Leonor,Qing Bi,Fei Lan,Pinchao Mei,Guo-Cheng Yuan,Christine G. Lian,Junmin Peng,Dongmei Cheng,Guangchao Sui,Ursula B. Kaiser,Yang Shi,Yujiang Geno Shi +15 more
TL;DR: Characterization of the LSD2 complex reveals that LSD2 forms active complexes with euchromatic histone methyltransferases G9a and NSD3 as well as cellular factors involved in transcription elongation, providing a possible molecular mechanism linking LSD2 to transcriptional regulation after initiation.
Journal ArticleDOI
The fundamental role of epigenetics in hematopoietic malignancies.
TL;DR: Altered DNA methylation patterns may serve as biomarkers for cancer detection, assessment of prognosis, and prediction of response to therapy and clinical trials using epigenetically targeted therapies have yielded promising results in hematopoietic malignancies.
References
More filters
Journal ArticleDOI
Crystal structure of the nucleosome core particle at 2.8 Å resolution
TL;DR: The X-ray crystal structure of the nucleosome core particle of chromatin shows in atomic detail how the histone protein octamer is assembled and how 146 base pairs of DNA are organized into a superhelix around it.
Journal ArticleDOI
Histone acetylation in chromatin structure and transcription
TL;DR: The amino termini of histones extend from the nucleosomal core and are modified by acetyltransferases and deacetylases during the cell cycle, which may direct histone assembly and help regulate the unfolding and activity of genes.
Journal ArticleDOI
Protein modules and signalling networks
TL;DR: This work highlights conserved protein domains that act as key regulatory participants in many of these different signalling pathways in multicellular organisms.
Journal ArticleDOI
Mitosis-specific phosphorylation of histone H3 initiates primarily within pericentromeric heterochromatin during G2 and spreads in an ordered fashion coincident with mitotic chromosome condensation.
Michael J. Hendzel,Y Wei,Michael A. Mancini,A. Van Hooser,Tamara A. Ranalli,Bill R. Brinkley,David P. Bazett-Jones,C D Allis +7 more
TL;DR: It is proposed that the singular phosphorylation of the amino-terminus of histone H3 may be involved in facilitating two key functions during mitosis: (1) regulate protein-protein interactions to promote binding of trans-acting factors that “drive” chromatin condensation as cells enter M-phase and (2) coordinate chromatin decondensation associated with M- phase.
Journal ArticleDOI
Histone acetylation and transcriptional regulatory mechanisms
TL;DR: Understanding of the causal relationship between histone acetylation and gene expression has been enhanced dramatically by the identification of proteins with intrinsic hist one acetylase and deacetylase activity, which led to a major paradigm shift in understanding of chromatin structure and transcription regulation.