Journal ArticleDOI
The preparation and characterization of synaptic vesicles of high purity.
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TLDR
Very pure preparations of synaptic vesicles have been obtained from guinea pig cerebral cortex and from the electromotor synapses of Torpedo marmorata by density gradient centrifugation in a zonal rotor followed by chromatography on columns of glass beads of controlled pore size, and showed that glycolipids and lysolecithin are present in negligible amounts.About:
This article is published in Brain Research.The article was published on 1976-06-11. It has received 203 citations till now. The article focuses on the topics: Synaptic vesicle & Vesicle.read more
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Molecular Anatomy of a Trafficking Organelle
Shigeo Takamori,Matthew Holt,Katinka Stenius,Edward A. Lemke,Mads Grønborg,Dietmar Riedel,Henning Urlaub,Stephan Schenck,Britta Brügger,Philippe Ringler,Shirley A. Müller,Burkhard Rammner,Frauke Gräter,Jochen S. Hub,Bert L. de Groot,Gottfried Mieskes,Yoshinori Moriyama,Jürgen Klingauf,Helmut Grubmüller,John E. Heuser,Felix T. Wieland,Reinhard Jahn +21 more
TL;DR: A model has been constructed that integrates all quantitative data and includes structural models of abundant proteins and, with the exception of the V-ATPase, contains numerous copies of proteins essential for membrane traffic and neurotransmitter uptake.
Journal ArticleDOI
Synapsin I (protein I), a nerve terminal-specific phosphoprotein. III. Its association with synaptic vesicles studied in a highly purified synaptic vesicle preparation.
TL;DR: It is shown, by immunocytochemical techniques at the light microscopic and electron microscopic levels, that synapsin I is present in the majority of, and possibly in all, nerve terminals, where it is primarily associated with synaptic vesicles.
Journal ArticleDOI
VAMP-1: a synaptic vesicle-associated integral membrane protein
TL;DR: Tryptic digestion of intact and lysed vesicles suggests that the protein faces the cytoplasm, where it may play a role in packaging, transport, or release of neurotransmitters.
Journal ArticleDOI
Signalling via ATP in the nervous system.
TL;DR: The cloning and molecular identification of two putative ATP receptors supports the previously established pharmacological receptor classifications and places into perspective the evidence for ATP as a neural signalling substance by examining sites of storage, release and hydrolysis, as well as potential actions and targets.
Journal ArticleDOI
Biochemistry, localization and functional roles of ecto-nucleotidases in the nervous system
TL;DR: The paper discusses the biochemical properties, cellular localization and functional properties of surface-located enzymes that hydrolyse nucleotides released from nervous tissue and indicates that 5'-nucleotidase appears to have a preferential glial allocation in the adult mammal.
References
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Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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A simple method for the isolation and purification of total lipides from animal tissues.
TL;DR: In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.
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A new and rapid colorimetric determination of acetylcholinesterase activity.
TL;DR: A photometric method for determining acetylcholinesterase activity of tissue extracts, homogenates, cell suspensions, etc., has been described and Kinetic constants determined by this system for erythrocyte eholinesterases are presented.
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Methods of Enzymatic Analysis
TL;DR: Methods of enzymatic analysis, Methods of enzymes analysis, the authors, Methods of enzyme analysis, enzymatics, methods of enzymes, and methods of analysis, method of enzymes.
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Phosphorus Assay in Column Chromatography
TL;DR: If the highest accuracy was not required, the following manipulations simplified and speeded multiple total phosphorus determinations on the eluates from column chromatographic separations.
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The separation of synaptic vesicles from nerve-ending particles (`synaptosomes')
Molecular Anatomy of a Trafficking Organelle
Shigeo Takamori,Matthew Holt,Katinka Stenius,Edward A. Lemke,Mads Grønborg,Dietmar Riedel,Henning Urlaub,Stephan Schenck,Britta Brügger,Philippe Ringler,Shirley A. Müller,Burkhard Rammner,Frauke Gräter,Jochen S. Hub,Bert L. de Groot,Gottfried Mieskes,Yoshinori Moriyama,Jürgen Klingauf,Helmut Grubmüller,John E. Heuser,Felix T. Wieland,Reinhard Jahn +21 more