The Salmonella invasin SipB induces macrophage apoptosis by binding to caspase-1
Reads0
Chats0
TLDR
The data demonstrate that SipB functions as an analog of the Shigella invasin IpaB, and functional inhibition of caspase-1 activity by acetyl-Tyr-Val-Ala-Asp-chloromethyl ketone blocks macrophage cytotoxicity, and macrophages lacking casp enzyme are not susceptible to Salmonella-induced apoptosis.Abstract:
Recently, Salmonella spp. were shown to induce apoptosis in infected macrophages. The mechanism responsible for this process is unknown. In this report, we establish that the Inv-Spa type III secretion apparatus target invasin SipB is necessary and sufficient for the induction of apoptosis. Purified SipB microinjected into macrophages led to cell death. Binding studies show that SipB associates with the proapoptotic protease caspase-1. This interaction results in the activation of caspase-1, as seen in its proteolytic maturation and the processing of its substrate interleukin-1β. Caspase-1 activity is essential for the cytotoxicity. Functional inhibition of caspase-1 activity by acetyl-Tyr-Val-Ala-Asp-chloromethyl ketone blocks macrophage cytotoxicity, and macrophages lacking caspase-1 are not susceptible to Salmonella-induced apoptosis. Taken together, the data demonstrate that SipB functions as an analog of the Shigella invasin IpaB.read more
Citations
More filters
Journal ArticleDOI
Pyroptosis: host cell death and inflammation
TL;DR: Pyroptosis, or caspase 1-dependent cell death, is inherently inflammatory, is triggered by various pathological stimuli, such as stroke, heart attack or cancer, and is crucial for controlling microbial infections.
Journal ArticleDOI
Molecular definitions of cell death subroutines: recommendations of the Nomenclature Committee on Cell Death 2012
Lorenzo Galluzzi,Ilio Vitale,Ilio Vitale,Ilio Vitale,John M. Abrams,Emad S. Alnemri,Eric H. Baehrecke,Mikhail V. Blagosklonny,Ted M. Dawson,Valina L. Dawson,Wafik S. El-Deiry,Simone Fulda,Eyal Gottlieb,Douglas R. Green,Michael O. Hengartner,Oliver Kepp,Oliver Kepp,Oliver Kepp,Richard A. Knight,Sharad Kumar,Sharad Kumar,Stuart A. Lipton,Xin Lu,Frank Madeo,Walter Malorni,Patrick Mehlen,Gabriel Núñez,Marcus E. Peter,Mauro Piacentini,David C. Rubinsztein,Yufang Shi,Hans-Uwe Simon,Peter Vandenabeele,Eileen White,Junying Yuan,Boris Zhivotovsky,Gerry Melino,Gerry Melino,Guido Kroemer +38 more
TL;DR: A functional classification of cell death subroutines is proposed that applies to both in vitro and in vivo settings and includes extrinsic apoptosis, caspase-dependent or -independent intrinsic programmed cell death, regulated necrosis, autophagic cell death and mitotic catastrophe.
Journal ArticleDOI
The Inflammasomes: Guardians of the Body
TL;DR: The role of NLRs, and in particular the inflammasomes, in the recognition of microbial and danger components and the role they play in health and disease are discussed.
Journal ArticleDOI
Apoptosis, pyroptosis, and necrosis: mechanistic description of dead and dying eukaryotic cells
Susan L. Fink,Brad T. Cookson +1 more
TL;DR: A wide variety of pathogenic microorganisms have been demonstrated to cause eukaryotic cell death, either as a consequence of infecting host cells or by producing toxic products, and apoptosis in many of these systems is characterized as apoptosis.
Journal ArticleDOI
Differential activation of the inflammasome by caspase-1 adaptors ASC and Ipaf.
Sanjeev Mariathasan,Kim Newton,Denise M. Monack,Domagoj Vucic,Dorothy French,Wyne P. Lee,Meron Roose-Girma,Sharon Erickson,Vishva M. Dixit +8 more
TL;DR: Interestingly, cell death triggered by stimuli that engage caspase-1 was ablated in macrophages lacking either ASC or Ipaf, suggesting a coupling between the inflammatory and cell death pathways.
References
More filters
Book
Antibodies: A Laboratory Manual
Ed Harlow,David P. Lane +1 more
TL;DR: A second edition of Antibodies: A Laboratory Manual is being published in September 2013, Revised, extended and updated by Edward Greenfield of the Dana-Farber Cancer Center, the material has been recast with extensive new information and new chapters have been added.
Journal ArticleDOI
Human ICE/CED-3 Protease Nomenclature
Emad S. Alnemri,David J. Livingston,Donald W. Nicholson,Guy S. Salvesen,Nancy A. Thornberry,Winnie W. Wong,Junying Yuan +6 more
TL;DR: A committee of several scientists who have been involved in the identification and characterization of these enzymes have formed a committee, with the objective of proposing a nomenclature for the human members of this protease family that is sensible and easy to use.
Journal ArticleDOI
Aromatic-dependent Salmonella typhimurium are non-virulent and effective as live vaccines.
TL;DR: The use of a tetracycline-resistance transposon, Tn10 (refs 5, 6), inserted in gene aroA to produce non-reverting, aromatic-requiring derivatives of virulent S. typhimurium strains were virtually non-virulent; their use as live vaccines conferred excellent protection against challenge with a virulent strain.
Journal ArticleDOI
Eukaryotic proteins expressed in Escherichia coli: an improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase.
Kun-Liang Guan,Jack E. Dixon +1 more
TL;DR: The introduction of the glycine kinker into fusion proteins allows for the cleavage of the fusion proteins while they are attached to the affinity resin resulting in a single step purification of the recombinant protein.
Book ChapterDOI
Apoptosis: Mechanisms and Roles in Pathology
Mark J. Arends,Andrew H. Wyllie +1 more
TL;DR: This chapter discusses the mechanisms and roles of apoptosis in pathology, and the arrival of neutrophil polymorphs permits digestion and phagocytosis of the constituents of the necrotic cells, but brings with it the risk of further tissue damage.