The synthesis of complex-type oligosaccharides. I. Structure of the lipid-linked oligosaccharide precursor of the complex-type oligosaccharides of the vesicular stomatitis virus G protein.
E. Li,Ira Tabas,Stuart Kornfeld +2 more
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The synthesis of the complex-type oligosaccharide unit of the vesicular stomatitis virus G protein is initiated by the en bloc transfer of a high molecular weight oligosACcharide from a lipid carrier to the nascent polypeptide.About:
This article is published in Journal of Biological Chemistry.The article was published on 1978-11-10 and is currently open access. It has received 398 citations till now. The article focuses on the topics: Oligosaccharide & Mannose.read more
Citations
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Journal ArticleDOI
Biological Roles of Glycans
TL;DR: It is time for the diverse functional roles of glycans to be fully incorporated into the mainstream of biological sciences, as they are no different from other major macromolecular building blocks of life, simply more rapidly evolving and complex.
Journal ArticleDOI
Inhibitors of the Biosynthesis and Processing of N-Linked Oligosaccharide Chains
TL;DR: A number of inhibitors have been identified that interfere with glycoprotein biosynthesis, processing, or transport, such as tunicamycin, tridecaptin, and flavomycin this paper.
Inhibitors of the biosynthesis and processing of n-linked
TL;DR: A number of glycoproteins have oligosaccharides linked to protein in a GlcNAc----asparagine bond that are either of the complex, the high-mannose or the hybrid structure and a number of inhibitors have been identified that interfere with glycoprotein biosynthesis, processing, or transport.
Journal ArticleDOI
The carbohydrate-binding specificity of pea and lentil lectins. Fucose is an important determinant.
TL;DR: Glycopeptide binding to lentil lectin-Sepharose was enhanced by the exposure of terminal N-acetylglucosamine residues on the glycopeptides, whereas binding to pea lectin -Sepharoses was enhancedBy the exposure to terminal mannose residues, and the differences in carbohydrate binding specificity were exploited to fractionate a mixture of [2-3H]mannose-labeled glycoproteins derived from mouse lymphoma cell gly
Book ChapterDOI
Primary structure of glycoprotein glycans: basis for the molecular biology of glycoproteins.
TL;DR: This chapter elaborates the primary structure and metabolism of the N -linked glycans, and presents the hypothesis that the catabolism of N -acetyl-lactosainine glycans starts by the action of endo-2-acetamido- 2-deoxy-β- D -glucosidases in liberating oligosaccharides that are then degraded by exoglycosidases.
References
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Journal ArticleDOI
Processing of high mannose oligosaccharides to form complex type oligosaccharides on the newly synthesized polypeptides of the vesicular stomatitis virus G protein and the IgG heavy chain.
TL;DR: Experiments performed with mouse LPCl cells which secrete IgG demonstrated a similar sequence of processing of the oligosaccharide units of this immunoglobulin, indicating that processing is probably a general phenomenon.
Journal ArticleDOI
The synthesis of complex-type oligosaccharides. II. Characterization of the processing intermediates in the synthesis of the complex oligosaccharide units of the vesicular stomatitis virus G protein.
Stuart Kornfeld,E. Li,Ira Tabas +2 more
TL;DR: To elucidate the sequence of processing, pulse-chase experiments were performed with virus-infected Chinese hamster ovary cells using labeled mannose, glucosamine, and galactose and demonstrated that processing is initiated by the rapid removal of 2 of the 3 glucose residues of the precursor oligosaccharide.
Journal ArticleDOI
Isolation of wheat germ agglutinin-resistant clones of Chinese hamster ovary cells deficient in membrane sialic acid and galactose.
TL;DR: Data suggest that the variant cells of Chinese hamster ovary cells fail to carry out specific glycosyltransferase reactions in vivo despite the fact that they possess the appropriate nucleotide sugars, glycoprotein and glycolipid acceptors, and glycosYLtransferases.
Journal ArticleDOI
Oligosaccharide chains are trimmed during synthesis of the envelope glycoprotein of vesicular stomatitis virus
TL;DR: These studies indicated that the large oligomannosyl core structures initially added to the glycoprotein were being "trimmed" by the removal of mannose residues prior to (and/or during) the addition of the branch chains terminating in sialic acid.
Book ChapterDOI
[2] Characterization of carbohydrate units of glycoproteins
TL;DR: This chapter discusses the methods by which several problems associated with carbohydrate portion of a glycoprotein can be approached, and it is evident that although a general approach to the study of the carbohydrate units of glycoproteins may be given, the details of the experiments are modified from protein to protein to meet the specific problems at hand.