The synthesis of complex-type oligosaccharides. I. Structure of the lipid-linked oligosaccharide precursor of the complex-type oligosaccharides of the vesicular stomatitis virus G protein.
E. Li,Ira Tabas,Stuart Kornfeld +2 more
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The synthesis of the complex-type oligosaccharide unit of the vesicular stomatitis virus G protein is initiated by the en bloc transfer of a high molecular weight oligosACcharide from a lipid carrier to the nascent polypeptide.About:
This article is published in Journal of Biological Chemistry.The article was published on 1978-11-10 and is currently open access. It has received 398 citations till now. The article focuses on the topics: Oligosaccharide & Mannose.read more
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A kinetic comparison of the processing and secretion of the alpha beta dimer and the uncombined alpha and beta subunits of chorionic gonadotropin synthesized by human choriocarcinoma cells.
TL;DR: Pulse-chase studies with [35S]methionine or [3H]mannose were carried out in order to compare free alpha, free beta, and the alpha beta dimer with regard to the kinetics of synthesis, N-linked oligosaccharide processing, and secretion and to determine the Kinetics of alpha-beta subunit combination.
Journal ArticleDOI
Characterization of human immunodeficiency virus type 2 envelope glycoproteins: dimerization of the glycoprotein precursor during processing.
TL;DR: Pulse-chase experiments indicated that gp300 is formed after synthesis of gp140 and before the detection of the mature external envelope glycoprotein, gp125, and these results were confirmed by using various inhibitors of glycosylation and inhibitors of trimming enzymes.
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Effect of glucosylation of lipid intermediates on oligosaccharide transfer in solubilized microsomes from Saccharomyces cerevisiae.
TL;DR: The rate of N-glycosylation in vitro is approximately 25-fold faster with Glc3Man9GlcNAc2-P- P-dolichol than with Man9Gl cNAc 2-P -P-D-dollichol, and the apparent Km value for the glucosylation species is 75 nM, while that for the unglucosylated glycolipid is 55 nM.
Journal ArticleDOI
The Biosynthetic Pathway of the Asparagine-Linked Oligosaccharides of Glycoproteins
TL;DR: This review deals with the structure and addition of the different types of oligosaccharides to asparagine residues in proteins.
Journal ArticleDOI
Structure of oligosaccharides and the linkage region between keratan sulfate and the core protein on proteoglycans from monkey cornea.
TL;DR: The structure of the linkage region for keratan sulfate on corneal proteoglycans is clearly derived from a complex type of N-linked glycoprotein oligosaccharide precursor, indicating that only the oligosACcharides that have been processed to the complex type are used as primers for synthesizing keratan sulphate chains.
References
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Detection of sugars on paper chromatograms.
TL;DR: Modifications are introduced, based on a test given by Feigl for reducing sugars, which eliminate the heating step, and in which the reagents are applied in organic solvents, thus removing the danger of migration of the sugar spots.
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Determination of aminosugar linkages in glycolipids by methylation: Aminosugar linkages of ceramide pentasaccharides of rabbit erythrocytes and of Forssman antigen☆
TL;DR: This method was successfully applied to analysis of aminosugar linkages in blood group B-active ceramide pentasaccharide from rabbit erythrocytes and in Forssman antigen of equine spleen.
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The role of polyprenol-linked sugars in glycoprotein synthesis.
C J Waechter,W J Lennarz +1 more
TL;DR: The chiral stationary phase for lipid-Linked Monosaccharides showed good chiral recognition ability towards various lipids, and this property has been generalized to other lipids as well.
Book ChapterDOI
[90] α-Mannosidase, β-N-acetylhexosaminidase, and β-galactosidase from jack bean meal
Yu-Teh Li,Su-Chen Li +1 more
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Structural studies of two ovalbumin glycopeptides in relation to the endo-beta-N-acetylglucosaminidase specificity.
T Tai,Katsuko Yamashita,M Ogata-Arakawa,Norio Koide,Takashi Muramatsu,Shintaro Iwashita,Y Inoue,Akira Kobata +7 more
TL;DR: The presence of the unsubstituted alpha-mannosyl residue linked at the C-3 position of the terminal mannose of Manbeta1 leads to 4 GlcNAcAsn core must be essential for the action of the enzyme.