The synthesis of complex-type oligosaccharides. I. Structure of the lipid-linked oligosaccharide precursor of the complex-type oligosaccharides of the vesicular stomatitis virus G protein.
E. Li,Ira Tabas,Stuart Kornfeld +2 more
Reads0
Chats0
TLDR
The synthesis of the complex-type oligosaccharide unit of the vesicular stomatitis virus G protein is initiated by the en bloc transfer of a high molecular weight oligosACcharide from a lipid carrier to the nascent polypeptide.About:
This article is published in Journal of Biological Chemistry.The article was published on 1978-11-10 and is currently open access. It has received 398 citations till now. The article focuses on the topics: Oligosaccharide & Mannose.read more
Citations
More filters
Journal ArticleDOI
Sequential assembly and polymerization of the polyprenol-linked pentasaccharide repeating unit of the xanthan polysaccharide in Xanthomonas campestris.
TL;DR: In this study, the stepwise assembly of the repeating pentasaccharide unit of xanthan is described and results from two-stage reactions showed that essentially pentasACcharide-P-P -polyprenol is polymerized.
Journal ArticleDOI
Isolation and characterization of mosquito cell membrane glycoproteins.
T.D. Butters,R.C. Hughes +1 more
TL;DR: Plasma membranes have been purified from an established cell line, Mos 20A of Aedes aegypti, and analysed for glycoprotein and polypeptide constituents by isoelectric focusing and sodium dodecyl sulphate polyacrylamide gel electrophoresis, indicating the presence of N-glycans and O-glycosidically linked N-acetylgalactosamine residues.
Journal ArticleDOI
N-Glycosylation of Yeast Proteins Characterization of the Solubilized Oligosaccharyl Transferase
TL;DR: The enzyme transferring the oligosaccharide from DolPP-(GlcNAc)2(Glc)3 to asparagine residues of glycoproteins has been solubilized from yeast membranes by extraction with detergents and a clear difference in Km values for the hexapeptide was observed with difference dolichol-linked sugar derivatives.
Book ChapterDOI
Structure and Assembly of Alphaviruses
TL;DR: The alphaviruses are enveloped animal viruses that belong to the family of Toga viruses and their molecular biology is almost entirely based on studies with two members of this virus group, the Semliki Forest Virus and the Sindbis virus.
Journal ArticleDOI
Relationship of the terminal sequences to the length of poly-N-acetyllactosamine chains in asparagine-linked oligosaccharides from the mouse lymphoma cell line BW5147. Immobilized tomato lectin interacts with high affinity with glycopeptides containing long poly-N-acetyllactosamine chains.
TL;DR: There is a relationship in these cells between poly-N-acetyllactosamine chain length and the degree and type of sialylation of these chains, and it is found that immobilized tomato lectin interacts with high affinity with glycopeptides containing three or more linear units of the repeating disaccharide and thereby allows for a separation of glycopesptides on the basis of the length of the chain.
References
More filters
Journal ArticleDOI
Detection of sugars on paper chromatograms.
TL;DR: Modifications are introduced, based on a test given by Feigl for reducing sugars, which eliminate the heating step, and in which the reagents are applied in organic solvents, thus removing the danger of migration of the sugar spots.
Journal ArticleDOI
Determination of aminosugar linkages in glycolipids by methylation: Aminosugar linkages of ceramide pentasaccharides of rabbit erythrocytes and of Forssman antigen☆
TL;DR: This method was successfully applied to analysis of aminosugar linkages in blood group B-active ceramide pentasaccharide from rabbit erythrocytes and in Forssman antigen of equine spleen.
Journal ArticleDOI
The role of polyprenol-linked sugars in glycoprotein synthesis.
C J Waechter,W J Lennarz +1 more
TL;DR: The chiral stationary phase for lipid-Linked Monosaccharides showed good chiral recognition ability towards various lipids, and this property has been generalized to other lipids as well.
Book ChapterDOI
[90] α-Mannosidase, β-N-acetylhexosaminidase, and β-galactosidase from jack bean meal
Yu-Teh Li,Su-Chen Li +1 more
Journal ArticleDOI
Structural studies of two ovalbumin glycopeptides in relation to the endo-beta-N-acetylglucosaminidase specificity.
T Tai,Katsuko Yamashita,M Ogata-Arakawa,Norio Koide,Takashi Muramatsu,Shintaro Iwashita,Y Inoue,Akira Kobata +7 more
TL;DR: The presence of the unsubstituted alpha-mannosyl residue linked at the C-3 position of the terminal mannose of Manbeta1 leads to 4 GlcNAcAsn core must be essential for the action of the enzyme.