The synthesis of complex-type oligosaccharides. I. Structure of the lipid-linked oligosaccharide precursor of the complex-type oligosaccharides of the vesicular stomatitis virus G protein.
E. Li,Ira Tabas,Stuart Kornfeld +2 more
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The synthesis of the complex-type oligosaccharide unit of the vesicular stomatitis virus G protein is initiated by the en bloc transfer of a high molecular weight oligosACcharide from a lipid carrier to the nascent polypeptide.About:
This article is published in Journal of Biological Chemistry.The article was published on 1978-11-10 and is currently open access. It has received 398 citations till now. The article focuses on the topics: Oligosaccharide & Mannose.read more
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Processing of carbohydrate units of glycoproteins. Characterization of a thyroid glucosidase.
Journal ArticleDOI
Carbohydrate-free carboxypeptidase Y is transferred into the lysosome-like yeast vacuole
TL;DR: In the presence of tunicamycin yeast cells synthesize a carbohydrate-free carboxypeptidase Y, which could be traced after metabolic labeling with [ 14 C]-phenylalanine, which was segregated into the vacuoles to the same extent as the intact glycoprotein.
Journal ArticleDOI
Biosynthesis of the erythrocyte anion transport protein.
W A Braell,H F Lodish +1 more
TL;DR: The biosynthesis of the erythrocyte anion transport protein (Band III) was studied in erythroid precursor cells obtained from the spleens of anemic mice, and resembles that of cotranslationally inserted proteins whose NH2-terminal portions are exposed on the exterior of the cell, like VSV glycoprotein, HLA-A antigens, and glycophorin.
Journal ArticleDOI
Sugar residues on proteins.
TL;DR: The current methods of structural elucidation such as monosaccharide sequence, intersugar bonds, and anomeric linkages in the carbohydrate moiety of glycoproteins will be reviewed, in order to develop an understanding of a specific function(s) of the carbohydrate in glyCoproteins.
Journal ArticleDOI
Effect of tunicamycin on the biosynthesis of the major human red cell sialoglycoprotein, glycophorin A, in the leukemia cell line K562.
TL;DR: The human continuous leukemia cell line K562 synthesizes and expresses on its surface the major red cell sialoglycoprotein, glycophorin A, and N-glycosylation is not necessary for externalization of glycoph orin A.
References
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TL;DR: Modifications are introduced, based on a test given by Feigl for reducing sugars, which eliminate the heating step, and in which the reagents are applied in organic solvents, thus removing the danger of migration of the sugar spots.
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Determination of aminosugar linkages in glycolipids by methylation: Aminosugar linkages of ceramide pentasaccharides of rabbit erythrocytes and of Forssman antigen☆
TL;DR: This method was successfully applied to analysis of aminosugar linkages in blood group B-active ceramide pentasaccharide from rabbit erythrocytes and in Forssman antigen of equine spleen.
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The role of polyprenol-linked sugars in glycoprotein synthesis.
C J Waechter,W J Lennarz +1 more
TL;DR: The chiral stationary phase for lipid-Linked Monosaccharides showed good chiral recognition ability towards various lipids, and this property has been generalized to other lipids as well.
Book ChapterDOI
[90] α-Mannosidase, β-N-acetylhexosaminidase, and β-galactosidase from jack bean meal
Yu-Teh Li,Su-Chen Li +1 more
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Structural studies of two ovalbumin glycopeptides in relation to the endo-beta-N-acetylglucosaminidase specificity.
T Tai,Katsuko Yamashita,M Ogata-Arakawa,Norio Koide,Takashi Muramatsu,Shintaro Iwashita,Y Inoue,Akira Kobata +7 more
TL;DR: The presence of the unsubstituted alpha-mannosyl residue linked at the C-3 position of the terminal mannose of Manbeta1 leads to 4 GlcNAcAsn core must be essential for the action of the enzyme.