The synthesis of complex-type oligosaccharides. I. Structure of the lipid-linked oligosaccharide precursor of the complex-type oligosaccharides of the vesicular stomatitis virus G protein.
E. Li,Ira Tabas,Stuart Kornfeld +2 more
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The synthesis of the complex-type oligosaccharide unit of the vesicular stomatitis virus G protein is initiated by the en bloc transfer of a high molecular weight oligosACcharide from a lipid carrier to the nascent polypeptide.About:
This article is published in Journal of Biological Chemistry.The article was published on 1978-11-10 and is currently open access. It has received 398 citations till now. The article focuses on the topics: Oligosaccharide & Mannose.read more
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Journal ArticleDOI
Glucose starvation leads in rat hepatoma cells to partially N-glycosylated glycoproteins including alpha 1-acid glycoproteins. Identification by endoglycolytic digestions in polyacrylamide gels.
H Baumann,G P Jahreis +1 more
TL;DR: Under conditions of limiting glycosylation, it is shown that non- or partially N-glycosylated proteins do not acquire additional oligosaccharide units after restoration of glucose although the proteins can undergo secondary modification and, in the case of the secretory proteins, can be exported.
Journal ArticleDOI
The biosynthesis of oligosaccharide-lipids. Partial purification and characterization of mannosyltransferase II.
TL;DR: The extent of purification of the enzyme and its resistance to inhibition by amphomycin strongly suggest that the enzyme catalyzes direct transfer from nucleotide-sugar to the oligosaccharide-lipid acceptor.
Book ChapterDOI
[39] Inhibition of the dolichol pathway of protein glycosylation
Ralph T. Schwarz,Roelf Datema +1 more
TL;DR: This chapter indicates how to inhibit glycosylation in vivo and in vitro, discusses the properties of the various inhibitors, and illustrates how to check whether inhibition of glycosolation is really achieved under the chosen experimental conditions.
Journal ArticleDOI
The high mannose oligosaccharides of Dictyostelium discoideum glycoproteins contain a novel intersecting N-acetylglucosamine residue.
TL;DR: Using a variety of techniques, including NMR spectroscopy and fast atom bombardment mass spectrometry, it is found that many of these oligosaccharides also contain an N-acetylglucosamine residue linked beta 1-4 to the mannose residue linked alpha 1-6 to the beta-linked core mannoses.
Journal ArticleDOI
Characterization of the N-linked oligosaccharides in glycoproteins synthesized by microfilariae of Dirofilaria immitis.
TL;DR: The results demonstrate that glycoproteins synthesized by microfilariae of D. immitis have unusual carbohydrate moieties and may lead to a better understanding of the specific roles of glycoprotein oligosaccharides in host-parasite interactions.
References
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Detection of sugars on paper chromatograms.
TL;DR: Modifications are introduced, based on a test given by Feigl for reducing sugars, which eliminate the heating step, and in which the reagents are applied in organic solvents, thus removing the danger of migration of the sugar spots.
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Determination of aminosugar linkages in glycolipids by methylation: Aminosugar linkages of ceramide pentasaccharides of rabbit erythrocytes and of Forssman antigen☆
TL;DR: This method was successfully applied to analysis of aminosugar linkages in blood group B-active ceramide pentasaccharide from rabbit erythrocytes and in Forssman antigen of equine spleen.
Journal ArticleDOI
The role of polyprenol-linked sugars in glycoprotein synthesis.
C J Waechter,W J Lennarz +1 more
TL;DR: The chiral stationary phase for lipid-Linked Monosaccharides showed good chiral recognition ability towards various lipids, and this property has been generalized to other lipids as well.
Book ChapterDOI
[90] α-Mannosidase, β-N-acetylhexosaminidase, and β-galactosidase from jack bean meal
Yu-Teh Li,Su-Chen Li +1 more
Journal ArticleDOI
Structural studies of two ovalbumin glycopeptides in relation to the endo-beta-N-acetylglucosaminidase specificity.
T Tai,Katsuko Yamashita,M Ogata-Arakawa,Norio Koide,Takashi Muramatsu,Shintaro Iwashita,Y Inoue,Akira Kobata +7 more
TL;DR: The presence of the unsubstituted alpha-mannosyl residue linked at the C-3 position of the terminal mannose of Manbeta1 leads to 4 GlcNAcAsn core must be essential for the action of the enzyme.