Tightly-orchestrated rearrangements govern catalytic center assembly of the ribosome.
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In this paper, the structure of six intermediates in late nuclear and cytoplasmic maturation of the large subunit of the ribosome was determined, revealing a tightly-choreographed sequence of protein and RNA rearrangements controlling the insertion of Rpl10.Abstract:
The catalytic activity of the ribosome is mediated by RNA, yet proteins are essential for the function of the peptidyl transferase center (PTC). In eukaryotes, final assembly of the PTC occurs in the cytoplasm by insertion of the ribosomal protein Rpl10 (uL16). We determine structures of six intermediates in late nuclear and cytoplasmic maturation of the large subunit that reveal a tightly-choreographed sequence of protein and RNA rearrangements controlling the insertion of Rpl10. We also determine the structure of the biogenesis factor Yvh1 and show how it promotes assembly of the P stalk, a critical element for recruitment of GTPases that drive translation. Together, our structures provide a blueprint for final assembly of a functional ribosome.read more
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Signal Transduction in Ribosome Biogenesis: A Recipe to Avoid Disaster.
TL;DR: Some of the well-studied pathways known to control ribosome biogenesis (PI3K-AKT-mTOR, RB-p53, MYC) are examined and how they may interact with some of the less well studied pathways (eIF2α kinase and RNA editing/splicing) in higher eukaryotes to regulate ribosomes biogenesis, assembly, and protein translation in a dynamic manner are examined.
Journal ArticleDOI
Mechanism of completion of peptidyltransferase centre assembly in eukaryotes.
Vasileios Kargas,Pablo Castro-Hartmann,Norberto Escudero-Urquijo,Kyle Dent,Christine Hilcenko,Carolin Sailer,Gertrude Zisser,Maria J Marques-Carvalho,Simone Pellegrino,Leszek Wawiórka,Stefan M.V. Freund,Jane L. Wagstaff,Antonina Andreeva,Alexandre Faille,Edwin Chen,Florian Stengel,Helmut Bergler,Alan J. Warren +17 more
TL;DR: The mechanism of peptidyltransferase centre (PTC) completion is presented that explains how integration of the last ribosomal proteins is coupled to release of the nuclear export adaptor Nmd3 and reveals how the central functional site of the ribosome is sculpted.
Journal ArticleDOI
Construction of the Central Protuberance and L1 Stalk during 60S Subunit Biogenesis.
Lukas Kater,Valentin Mitterer,Matthias Thoms,Matthias Thoms,Jingdong Cheng,Otto Berninghausen,Roland Beckmann,Ed Hurt +7 more
TL;DR: Two nuclear pre-60S intermediates were discovered that represent previously unknown states after Rea1-mediated removal of the Ytm1-Erb1 complex and reveal how the L1 stalk develops from a pre-mature nucleolar to a mature-like nucleoplasmic state.
Journal ArticleDOI
Systematic Review of Plant Ribosome Heterogeneity and Specialization.
Federico Martinez-Seidel,Federico Martinez-Seidel,Olga Beine-Golovchuk,Yin-Chen Hsieh,Joachim Kopka +4 more
TL;DR: A data analysis pipeline is proposed that infers the heterogeneity of ribosome complexes and deviations from canonical structural compositions linked to stress events and can be extrapolated and enhanced by combination with other high-throughput methodologies, such as proteomics.
Journal ArticleDOI
The GTPase Nog1 co-ordinates the assembly, maturation and quality control of distant ribosomal functional centers.
Purnima Klingauf-Nerurkar,Ludovic C Gillet,Daniela Portugal-Calisto,Michaela Oborská-Oplová,Michaela Oborská-Oplová,Martin Jäger,Olga T. Schubert,Agnese Pisano,Cohue Peña,Sanjana Rao,Martin Altvater,Yiming Chang,Ruedi Aebersold,Ruedi Aebersold,Vikram Govind Panse +14 more
TL;DR: It is revealed that sequential ATPase and GTPase activities license release factors Rei1 and Yvh1 to trigger Arx1 and Mrt4 removal and Nog1 co-ordinates the assembly, maturation and quality control of distant functional centers during ribosome formation.
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