Journal ArticleDOI
Uncovering leaf rust responsive miRNAs in wheat (Triticum aestivum L.) using high-throughput sequencing and prediction of their targets through degradome analysis
Dhananjay Kumar,Summi Dutta,Dharmendra Singh,Dharmendra Singh,Kumble Vinod Prabhu,Manish Kumar,Kunal Mukhopadhyay +6 more
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TLDR
The obtained results indicate that wheat isolines employ diverse arrays of miRNAs that modulate their target genes during compatible and incompatible interaction, and contribute to increase knowledge on roles of microRNA in wheat–leaf rust interactions.Abstract:
Deep sequencing identified 497 conserved and 559 novel miRNAs in wheat, while degradome analysis revealed 701 targets genes. QRT-PCR demonstrated differential expression of miRNAs during stages of leaf rust progression. Bread wheat (Triticum aestivum L.) is an important cereal food crop feeding 30 % of the world population. Major threat to wheat production is the rust epidemics. This study was targeted towards identification and functional characterizations of micro(mi)RNAs and their target genes in wheat in response to leaf rust ingression. High-throughput sequencing was used for transcriptome-wide identification of miRNAs and their expression profiling in retort to leaf rust using mock and pathogen-inoculated resistant and susceptible near-isogenic wheat plants. A total of 1056 mature miRNAs were identified, of which 497 miRNAs were conserved and 559 miRNAs were novel. The pathogen-inoculated resistant plants manifested more miRNAs compared with the pathogen infected susceptible plants. The miRNA counts increased in susceptible isoline due to leaf rust, conversely, the counts decreased in the resistant isoline in response to pathogenesis illustrating precise spatial tuning of miRNAs during compatible and incompatible interaction. Stem-loop quantitative real-time PCR was used to profile 10 highly differentially expressed miRNAs obtained from high-throughput sequencing data. The spatio-temporal profiling validated the differential expression of miRNAs between the isolines as well as in retort to pathogen infection. Degradome analysis provided 701 predicted target genes associated with defense response, signal transduction, development, metabolism, and transcriptional regulation. The obtained results indicate that wheat isolines employ diverse arrays of miRNAs that modulate their target genes during compatible and incompatible interaction. Our findings contribute to increase knowledge on roles of microRNA in wheat-leaf rust interactions and could help in rust resistance breeding programs.read more
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Journal ArticleDOI
The progress of leaf rust research in wheat.
TL;DR: This review summarizes available information on different aspects of the pathogen Pt, genetics/genomics of leaf rust resistance in wheat including cloning and characterization of Lr genes and epigenetic regulation of disease resistance.
Journal ArticleDOI
Small RNA discovery in the interaction between barley and the powdery mildew pathogen.
Matt Hunt,Sagnik Banerjee,Priyanka Surana,Meiling Liu,Greg Fuerst,Sandra M. Mathioni,Blake C. Meyers,Blake C. Meyers,Dan Nettleton,Roger P. Wise +9 more
TL;DR: These data suggest that Bgh sRNAs regulate gene expression in metabolism, translation-related, and pathogen effectors, and both barley and Bgh control metabolism and infection-related responses via the specific accumulation and targeting of genes via s RNAs.
Journal ArticleDOI
Identification of Regulatory Networks of MicroRNAs and Their Targets in Response to Colletotrichum gloeosporioides in Tea Plant (Camellia sinensis L.).
Anburaj Jeyaraj,Xuewen Wang,Xuewen Wang,Shuangshuang Wang,Shengrui Liu,Ran Zhang,Ailin Wu,Chaoling Wei +7 more
TL;DR: Gene Ontology and metabolism pathway analysis revealed that most of the target genes were involved in the regulation of auxin pathway, ROS scavenging pathway, salicylic acid mediated pathway, receptor kinases, and transcription factors for plant growth and development as well as stress responses in tea plant against C. gloeosporioides stress.
Journal ArticleDOI
miR477 targets the phenylalanine ammonia-lyase gene and enhances the susceptibility of the tea plant (Camellia sinensis) to disease during Pseudopestalotiopsis species infection
TL;DR: The results implied that Csn-miR477 might act as a negative regulator in pathogen-infected tea plants by inhibiting the expression of its target, PAL, and that C Sn- miR477 is a candidate miRNA for improving the adaptation of tea plant to disease.
Journal ArticleDOI
Analysis of miRNAs in Two Wheat Cultivars Infected With Puccinia striiformis f. sp. tritici
TL;DR: This study analyzed the small RNA profiles of two cultivars that exhibit different reactions to stripe rust infection, providing evidence for miRNA-mediated regulation during infection and providing novel information on the yet-uncharacterized roles for miRNAs in the wheat-stripe rust pathosystem.
References
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