Vectored expression of the broadly neutralizing antibody FI6 in mouse airway provides partial protection against a new avian influenza A virus, H7N9.
Maria P. Limberis,Trina Racine,Darwyn Kobasa,Yan Li,George Fu Gao,Gary P. Kobinger,Gary P. Kobinger,James M. Wilson +7 more
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TLDR
H7N9 is currently contained in Southeastern China, and the majority of human cases of infection have reported direct exposure to poultry, while no fatalities have been reported.Abstract:
As of 4 July 2013, there have been 134 confirmed cases of human infection and 43 fatalities with a new avian influenza A virus (H7N9) ([1][1]). H7N9 is currently contained in Southeastern China, and the majority of human cases of infection have reported direct exposure to poultry ([2][2]). While noread more
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Protection against dengue disease by synthetic nucleic acid antibody prophylaxis/immunotherapy
Seleeke Flingai,Emily M. Plummer,Ami Patel,Sujan Shresta,Janess M. Mendoza,Kate E. Broderick,Niranjan Y. Sardesai,Karuppiah Muthumani,David B. Weiner +8 more
TL;DR: It is demonstrated that simple intramuscular delivery by electroporation of synthetic DNA plasmids engineered to express modified human nAbs against multiple DENV serotypes confers protection against DENV disease and prevents antibody-dependent enhancement of disease in mice.
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Adeno-associated virus 9-mediated airway expression of antibody protects old and immunodeficient mice against influenza virus.
Virginie S. Adam,Marco Crosariol,Sachin Kumar,Moyar Q. Ge,Sarah E. Czack,Soumitra Roy,Angela Franciska Haczku,Anna Tretiakova,James M. Wilson,Maria P. Limberis +9 more
TL;DR: It is proposed that airway-directed AAV9 passive immunization against airborne infectious agents may be beneficial in elderly and immunocompromised patients, for whom there still exists an unmet need for effective vaccination against influenza.
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Intramuscular Adeno-Associated Virus-Mediated Expression of Monoclonal Antibodies Provides 100% Protection Against Ebola Virus Infection in Mice.
Laura P. van Lieshout,Geoff Soule,Debra Sorensen,Kathy L. Frost,Shihua He,Kevin Tierney,David Safronetz,Stephanie A. Booth,Gary P. Kobinger,Gary P. Kobinger,Xiangguo Qiu,Sarah K. Wootton +11 more
TL;DR: It is demonstrated that AAV-mediated expression of nonneutralizing mAb 5D2 or 7C9 confers 100% protection against mouse-adapted Ebola virus infection, while neutralizing m Ab 2G4 was 83% protective.
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Genetic Modification of the Lung Directed Toward Treatment of Human Disease
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Human Infection with a Novel Avian-Origin Influenza A (H7N9) Virus
Rongbao Gao,Bin Cao,Yunwen Hu,Zijian Feng,Dayan Wang,Wanfu Hu,Jian Chen,Zhijun Jie,Haibo Qiu,Ke Xu,Xuewei Xu,Hongzhou Lu,Wenfei Zhu,Zhancheng Gao,Nijuan Xiang,Yinzhong Shen,Zebao He,Y. Gu,Zhiyong Zhang,Yi Yang,Xiang Zhao,Lei Zhou,Xiaodan Li,Shumei Zou,Ye Zhang,Xiyan Li,Lei Yang,Junfeng Guo,Jie Dong,Qun Li,Libo Dong,Yun Zhu,Tian Bai,Shiwen Wang,Pei Hao,Weizhong Yang,Yanping Zhang,Jun Han,Hongjie Yu,Dexin Li,George F. Gao,Guizhen Wu,Yu Wang,Zhenghong Yuan,Yuelong Shu +44 more
TL;DR: Novel reassortant H7N9 viruses were associated with severe and fatal respiratory disease in three patients, and all three patients died.
Journal ArticleDOI
A neutralizing antibody selected from plasma cells that binds to group 1 and group 2 influenza A hemagglutinins
Davide Corti,Jarrod Voss,Steven J. Gamblin,Giosiana Codoni,Annalisa Macagno,David Jarrossay,Sebastien G. Vachieri,Debora Pinna,Andrea Minola,Fabrizia Vanzetta,Chiara Silacci,Blanca Fernandez-Rodriguez,Gloria Agatic,Siro Bianchi,Isabella Giacchetto-Sasselli,Lesley J. Calder,Federica Sallusto,Patrick J. Collins,Lesley F. Haire,Nigel J. Temperton,Johannes P. M. Langedijk,John J. Skehel,Antonio Lanzavecchia +22 more
TL;DR: An antibody able to broadly neutralize both group 1 and group 2 influenza A viruses—and its target epitope—are identified and may be used for passive protection and to inform vaccine design because of its broad specificity and neutralization potency.
Journal ArticleDOI
Epidemiology of Human Infections with Avian Influenza A(H7N9) Virus in China
Qun Li,Lei Zhou,Minghao Zhou,Zhiping Chen,Furong Li,Huanyu Wu,Nijuan Xiang,Enfu Chen,Fenyang Tang,Dayan Wang,Ling Meng,Zhiheng Hong,Wenxiao Tu,Yang Cao,Leilei Li,Fan Ding,Bo Liu,Mei Wang,Rongheng Xie,Rongbao Gao,Xiaodan Li,Tian Bai,Shumei Zou,Jun He,Jiayu Hu,Yangting Xu,Chengliang Chai,Shiwen Wang,Yongjun Gao,Lianmei Jin,Yanping Zhang,Huiming Luo,Hongjie Yu,Jianfeng He,Qi Li,Xianjun Wang,Lidong Gao,Xinghuo Pang,Guohua Liu,Yansheng Yan,Hui Yuan,Yuelong Shu,Weizhong Yang,Yu Wang,Fan Wu,Timothy M. Uyeki,Zijian Feng +46 more
TL;DR: Most persons with confirmed H7N9 virus infection had severe lower respiratory tract illness, were epidemiologically unrelated, and had a history of recent exposure to poultry.
Journal ArticleDOI
H7N9 Influenza Viruses Are Transmissible in Ferrets by Respiratory Droplet
Qianyi Zhang,Qianyi Zhang,Jianzhong Shi,Guohua Deng,Jing Guo,Xianying Zeng,Xijun He,Huihui Kong,Chunyang Gu,Chunyang Gu,Xuyong Li,Jinxiong Liu,Guojun Wang,Guojun Wang,Yan Chen,Liling Liu,Libin Liang,Yuanyuan Li,Jun Fan,Jinliang Wang,Wenhui Li,Lizheng Guan,Qimeng Li,Qimeng Li,Huanliang Yang,Pucheng Chen,Li Jiang,Yuntao Guan,Xiaoguang Xin,Yongping Jiang,Guobin Tian,Xiurong Wang,Chuanling Qiao,Chengjun Li,Zhigao Bu,Hualan Chen,Hualan Chen +36 more
TL;DR: A large survey of H7N9 influenza viruses in China reveals a spectrum of mutation and virulence, with one virus isolated from humans was highly transmissible in ferrets by respiratory droplet and another caused up to 30% body weight loss in mice.
Journal ArticleDOI
Biological features of novel avian influenza A (H7N9) virus
Jiangfang Zhou,Dayan Wang,Rongbao Gao,Baihui Zhao,Jingdong Song,Xian Qi,Yanjun Zhang,Yonglin Shi,Lei Yang,Wenfei Zhu,Tian Bai,Kun Qin,Yu Lan,Shumei Zou,Junfeng Guo,Jie Dong,Libo Dong,Ye Zhang,Hejiang Wei,Xiaodan Li,Jian Lu,Liqi Liu,Xiang Zhao,Xiyan Li,Weijuan Huang,Le-ying Wen,Hong Bo,Li Xin,Y. Chen,Cuilin Xu,Yuquan Pei,Yue Yang,Xiaodong Zhang,Shiwen Wang,Zijian Feng,Jun Han,Weizhong Yang,George F. Gao,Guizhen Wu,Dexin Li,Yu Wang,Yuelong Shu +41 more
TL;DR: It is shown that the emerging H7N9 avian influenza virus poses a potentially high risk to humans, and current seasonal vaccination could not provide protection.