X-ray photon correlation spectroscopy of protein dynamics at nearly diffraction-limited storage rings
TLDR
How to optimize experimental setups to make BioXPCS measurements feasible at new generation synchrotron sources is explained.About:
This article is published in IUCrJ.The article was published on 2019-09-01 and is currently open access. It has received 37 citations till now. The article focuses on the topics: Photon energy & Length scale.read more
Citations
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Journal ArticleDOI
Potential and limits of a colloid approach to protein solutions.
TL;DR: A critically discuss the exploitation of colloid science concepts to better understand protein solutions and discusses current shortcomings in the ability to correctly reproduce and predict the structural and dynamic properties of protein solutions based on such a colloid approach.
Journal ArticleDOI
X-ray-Based Techniques to Study the Nano-Bio Interface
Carlos Sanchez-Cano,Ramon A. Alvarez-Puebla,John M. Abendroth,Tobias Beck,Robert H. Blick,Yuan Cao,Frank Caruso,Indranath Chakraborty,Henry N. Chapman,Chunying Chen,Bruce E. Cohen,Andre L.C. Conceição,David P. Cormode,Daxiang Cui,Kenneth A. Dawson,Gerald Falkenberg,Chunhai Fan,Neus Feliu,Mingyuan Gao,E. Gargioni,Claus C. Glüer,Florian Grüner,Moustapha Hassan,Yong Hu,Yalan Huang,Samuel Huber,Nils Huse,Yanan Kang,Ali Khademhosseini,Thomas F. Keller,Christian Körnig,Nicholas A. Kotov,Dorota Koziej,Xing-Jie Liang,Beibei Liu,Sijin Liu,Yang Liu,Ziyao Liu,Luis M. Liz-Marzán,Xiaowei Ma,Andres Machicote,Wolfgang Maison,Adrian P. Mancuso,Adrian P. Mancuso,Saad Megahed,Bert Nickel,Ferdinand Otto,Cristina Palencia,Sakura Pascarelli,Arwen R. Pearson,Oula Penate-Medina,Bing Qi,Joachim O. Rädler,Joseph J. Richardson,Axel Rosenhahn,Kai Rothkamm,Michael Rübhausen,Milan K. Sanyal,Raymond E. Schaak,Heinz Peter Schlemmer,Marius Schmidt,Oliver Schmutzler,Theo Schotten,Florian Schulz,A. K. Sood,Kathryn Spiers,Theresa Staufer,Dominik M. Stemer,Andreas Stierle,Xing Sun,Xing Sun,Gohar Tsakanova,Paul S. Weiss,Horst Weller,Fabian Westermeier,Ming Xu,Huijie Yan,Yuan Zeng,Ying Zhao,Yuliang Zhao,Dingcheng Zhu,Ying Zhu,Wolfgang J. Parak,Wolfgang J. Parak +83 more
TL;DR: X-ray-based analytics are routinely applied in many fields, including physics, chemistry, materials science, and engineering as discussed by the authors, but the full potential of such techniques in the life sciences and medicine has not yet been fully exploited.
Journal ArticleDOI
From Femtoseconds to Hours—Measuring Dynamics over 18 Orders of Magnitude with Coherent X-rays
TL;DR: X-ray photon correlation spectroscopy (XPCS) as mentioned in this paper enables the study of sample dynamics between micrometer and atomic length scales, and it benefits from the increased brilliance of the next-generation synchrotron radiation and Free-Electron Laser (FEL) sources.
Journal ArticleDOI
Microscopic Dynamics of Liquid-Liquid Phase Separation and Domain Coarsening in a Protein Solution Revealed by X-Ray Photon Correlation Spectroscopy
Anita Girelli,Hendrik Rahmann,Nafisa Begam,Anastasia Ragulskaya,Mario Reiser,Sivasurender Chandran,Sivasurender Chandran,Fabian Westermeier,Michael Sprung,Fajun Zhang,Christian Gutt,Frank Schreiber +11 more
TL;DR: In this article, the authors used x-ray photon correlation spectroscopy to determine the liquid-liquid phase separation dynamics of a model protein solution upon low temperature quenches and find distinctly different dynamical regimes.
Journal ArticleDOI
Towards molecular movies with X-ray photon correlation spectroscopy
Fivos Perakis,Christian Gutt +1 more
TL;DR: This perspective article highlights research opportunities and challenges in probing structural dynamics of molecular systems using X-ray Photon Correlation Spectroscopy (XPCS) and provides practical tips for designing and estimating feasibility of XPCS experiments as well as on detecting and mitigating radiation damage.
References
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TL;DR: Positive results of crowding include enhancing the collapse of polypeptide chains into functional proteins, the assembly of oligomeric structures and the efficiency of action of some molecular chaperones and metabolic pathways.
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Insights into phase transition kinetics from colloid science
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Accuracy of molecular mass determination of proteins in solution by small-angle X-ray scattering
TL;DR: In this article, the molecular mass (MM) of a monodisperse protein solution is calculated from the extrapolated scattering intensity at zero angle I(0), which provides valuable information about the oligomeric state and absence of unspecific aggregation.
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Interplay between Spinodal Decomposition and Glass Formation in Proteins Exhibiting Short-Range Attractions
TL;DR: It is shown that quenches below a temperature Ta lead to gel formation as a result of a local arrest of the protein-dense phase during spinodal decomposition.