Jo Vandesompele

TL;DR: Expression of miR-629-3p, mi-223-3P, and mi-142-3 p is increased in sputum of patients with severe asthma and is linked to neutrophilic airway inflammation, suggesting that these miRNAs contribute to this asthma inflammatory phenotype.

TL;DR: It is concluded that an activating mutation within the ALK kinase domain is sufficient to induce neuroblastoma development, and ALK inhibitors show promise for treating human neuroblastomas harboring ALK mutations.

TL;DR: Single mismatches located >5 bp from the 3' end have a moderate effect on qPCR amplification and can be tolerated, and this finding, together with the concentration independence for single mismatches and the complete blocking of the PCR reaction for ≥4 mismatches, can help to chart mismatch behavior inqPCR reactions and increase the rate of successful primer design for sequences with a high SNP density or for homologous regions of sequence.

TL;DR: The power of the previously described global meannormalization method is summarized in comparison to the multiple reference gene normalization method using the most stably expressed small RNA controls and a modified global mean normalization strategy based on the attribution of equal weight to each individual miRNA during normalization is compared.

TL;DR: This unique dataset adds power to ongoing and future gene expression studies in neuroblastoma and will facilitate the identification of molecular targets for novel therapies and could prove useful for the further study of human sympathoadrenal biogenesis.