Jo Vandesompele

TL;DR: It is found that a robust weighted least-squares approach is highly advisable, yet may also suffer from an inflated false-positive rate and the proposed assessments are also applicable to other analyses, such as the comparison of results obtained from qPCR and dPCR.

TL;DR: This work proposes a novel method that unites preprocessing and differential expression analysis in a single statistical model that provides a rigorous way for handling undetermined Cq values and shows that this method outperforms traditional RT-qPCR differential expressionAnalysis pipelines in the presence of undetermined values, both in terms of accuracy and precision.

TL;DR: A novel ESC m(i)RNA signature stratifies neuroblastomas with poor prognosis, enabling the identification of therapy-resistant tumors and warrants for drug design targeted at FOXM1 or key components controlling this pathway.

TL;DR: In muscle biopsies from dermatomyositis and polymyositis patients, NFAT5 colocalized with HDAC6, while in IBM, this was often absent, and disturbed myogenicNFAT5 physiology could possibly explain deleterious effects on muscle regeneration in myositis.

TL;DR: It is confirmed that in mouse tissues, EREs are broadly applicable reference targets for RT-qPCR normalization, provided that the RNA samples undergo a thorough DNase treatment.