Robert J. Lefkowitz

TL;DR: The recently cloned human beta-adrenergic cDNA and several mutated forms expressed in Xenopus laevis oocytes are validated and the utility of the Xenopus oocyte system for studying functional and regulatory properties of receptors coupled to adenylate cyclase is documented, and the possibility that elements in the 5' untranslated region of the beta 2- adrenergic receptor RNA may regulate its translation in vivo is suggested.

TL;DR: Long term agonist-induced desensitization of alpha 2AAR was found to be due in part to a decrease in the amount of cellular Gi, which was not dependent on receptor third loop phosphorylation sites.

TL;DR: It is suggested that S1,2,3 interacts with Gi as well as Gs, and that receptor:G protein coupling requires the concerted participation of multiple cytoplasmic receptor domains.

TL;DR: Recently developed radioligand binding techniques permit direct investigation of the alpha- and beta-adrenergic receptors for catecholamines in a wide variety of tissues, providing fresh insights into the mechanisms by which endogenous catecholsamines and other hormones regulate the properties of the adrenergic receptors and, in turn, control tissue sensitivity to catecholic action.

TL;DR: The data suggest that alpha-adrenergic receptors can be directly identified and studied by [3H]dihydroergocryptine binding, and that catecholamines that are devoid of alpha- adrenergic physiological activity do not compete for the binding sites.