Gandhi Institute of Technology and Management

About: Gandhi Institute of Technology and Management is a education organization based out in Visakhapatnam, Andhra Pradesh, India. It is known for research contribution in the topics: Computer science & Heat transfer. The organization has 3392 authors who have published 4043 publications receiving 29139 citations. The organization is also known as: GITAM & GITAM College.

Enhanced Alkaloid Production From Cell Culture System of Catharanthus Roseus in Combined Effect of Nutrient Salts, Sucrose and Plant Growth Regulators

Abstract: Callus and biomass culture of Catharanthus roseus L. were established to check for the presence of total alkaloid and its subsequent yield. Various treatments like strength of nutrient salts, sucrose concentrations and combinations of plant growth regulators (PGR’s) were applied to both MS and B5 in agar as well as suspension medium to test their effects on enhanced alkaloid content and its yield. There was no significant difference in any of the observable parameters of fresh wt, dry wt, alkaloid content, production, productivity and yield if the culture were treated similarly in both types of media formulations (MS or B5 salts). Physical state (agar solidified or the liquid suspension) of the medium had significant effect on all the parameters particular on fresh wt, alkaloid content and yields. Although, the fresh wt. and dry wt. of biomass in suspension culture was 2-3 times less than that of callus obtained from agar medium. However, the alkaloid content and yield was 2-3 times higher in suspension culture compared to agar medium in similar treatments. The highest alkaloid content observed was 5.67mg/g dwt in B5 suspension medium containing 3% sucrose and modified with 0.5mg/l 2,4-Dichlorophenoxy acetic acid (2,4-D) + 1 mg/l Kinetin (KIN) + 2mg/l α- naphthalene acetic acid (NAA). The combined effects of these factors on the enhanced production of total alkaloids were expected to contain higher yield of anticancer vinblastine and vincristine in the cell suspension culture system.

Zinc oxide eugenol and Endoflas pulpectomy in primary molars: 24-month clinical and radiographic evaluation.

Abstract: Context: Despite modern advancement in material and technical aspect, management of infected primary molars is of prime concern in pediatric endodontics. An effective root canal material plays the major role in achieving the fluid impervious seal by defending against variant microflora and maintaining the tooth in function for longer duration. Aims: This study aims to evaluate and compare the success of endoflas as root canal filling material in infected primary molars with zinc oxide eugenol (ZOE). Materials and Methods: Primary molars with necrotic pulp in healthy, cooperative children were selected. Ethical clearance and informed consent was obtained. Standardized pulpectomy procedure was done and root canals were filled with either ZOE or endoflas. Further follow-up with clinical and radiographic evaluation was carried at 0, 3, 6, 12, and 24 months. The findings obtained were statistically analyzed using Chi-square test. Results: Endoflas showed acceptable results as root canal filling material in primary molars even at 2-year follow-up, though overfilling of root canals led to low success rate compared to teeth with combined optimal and under fillings. There was no significant difference between the two materials (P > 0.05). Conclusions: Endoflas could be a potential alternative to ZOE for preserving infected primary molars.

Chemical Reaction and Radiation Effects on Non-Newtonian Fluid Flow over a Stretching Sheet with Non-Uniform Thickness and Heat Source

Abstract: The principle priority of this study is to inspect the performance of two dimensional chemically reacting non-Newtonian fluid bearing Soret, Dufour, thermal radiation, heat source and slip effects. The flow is prompted by a slendering surface with variable thickness. Casson and Williamson fluid models are incorporated in this discussion. Governing equations are evolved and converted into ordinary differential equations using similarity transformations. We adopted homotopy analysis method (HAM) to pick up the solutions. The graphical and tabular results for velocity, temperature, concentration, skin friction factor, local Nusselt number and Sherwood number are secured for both Casson and Williamson fluids. The correspondence between the acquired and previous results reveals that they are in good correlation. It is found that there is a significant increase in the thermal boundary layer thickness when the strength of the Dufour number is increased.

Epigenetic Mechanisms: Role in Hematopoietic Stem Cell Lineage Commitment and Differentiation.

Abstract: Major breakthroughs in the last several decades have contributed to our knowledge of the genetic regulation in development. Although epigenetics is not a new concept, unfortunately, the role of epigenetics has not come to fruition in the past. But the field of epigenetics has exploded within the past decade. Now, growing evidences show a complex network of epigenetic regulation in development. The epigenetic makeup of a cell, tissue or individual is much more complex than their genetic complement. Epigenetic modifications are more important for normal development by maintaining the gene expression pattern in tissue- and context-specific manner. Deregulation of epigenetic mechanism can lead to altered gene expression and its function, which result in altered tissue specific function of cells and malignant transformation. Epigenetic modifications directly shape Hematopoietic Stem Cell (HSC) developmental cascades, including their maintenance of self-renewal and multilineage potential, lineage commitment, and aging. Hence, there is a growing admiration for epigenetic players and their regulatory function in haematopoiesis. Epigenetic mechanisms underlying these modifications in mammalian genome are still not completely understood. This review mainly explains 3 key epigenetics mechanisms including DNA methylation, histone modifications and non-coding RNAs inference in hematopoietic lineage commitment and differentiation.

MHD Casson fluid in a suspension of convective conditions and cross diffusion across a surface of paraboloid of revolution

Abstract: In this paper, we analyzed the mathematical model of the convective conditions on Casson fluid across a Paraboloid of Revolution (PR) in the presence of pertinent parameters like magnetic field parameter, Soret, and Dufour. We used R-K-Felhberg-integration scheme based shooting technique to solve the modified governing equations and discussed the appearance of curious parameters on the profiles velocity, temperature and concentration profiles in three cases n = 0, n = 0.5 and n = 1 with the aid of plots. We found that the profiles of velocity, temperature, and concentration are non-uniform for all these three cases. Also, we have taken the help of tables to explore the skin friction coefficient, local Nusselt and Sherwood numbers, which are useful for the purpose of engineering interest. It is noticed that the cross diffusion helps to control the thermal, diffusion and momentum boundary layers for all the three cases. The mixed convective conditions are useful in improving the heat and mass transport phenomena. We also validated the present methodology with already existing methodologies under some limited cases.