Kadi Sarva Vishwavidyalaya

About: Kadi Sarva Vishwavidyalaya is a education organization based out in Gandhinagar, Gujarat, India. It is known for research contribution in the topics: Matrix (chemical analysis) & Mass spectrometry. The organization has 131 authors who have published 158 publications receiving 1343 citations.

Development and characterization of microemulsion based topical gel of essential oil of clove (Syzygium aromaticum) for superficial fungal infections

Abstract: A widespread of superficial fungal infections deals with concerns related to current therapeutic regimen such as drug resistance and adverse events associated with the same which leads exploration of natural oils as an antifungal agent. The aim of present work is to the development of clove oil loaded microemulsion based gel for treatment of superficial fungal infections. The microemulsion based gel was prepared by phase titration method and optimized using D-optimal design considering globule size, drug permeation and drug retention on skin as critical quality attributes. The MIC and zone of inhibition of clove oil was found to be 2.2 mg/ml and 38 mm respectively. A pale to yellowish transparent microemulsion had a globule size, zeta-potential and PDI of 14.41 nm, 0.73 and 0.0113 respectively indicating a stable microemulsion. A clove oil loaded microemulsion based gel (CLMBG) with a pH of 6.27 and viscosity of 12.87 m.pas/sec exhibited a comparable texture profile to marketed preparation. The drug release of the CLMBG with a drug content of 102.6 ± 4% in acetate buffer pH 5.5 was 98.5 ± 0.35% ensuring complete drug release from the formulation. Ex-vivo drug permeation study and skin irritation study dictated the retention of drug at site of action and the formulation to be non-irritant. The antifungal study proved the formulation to have similar efficacy as the marketed product (clobet gel). The stability study indicated the product to be safe, efficacious and stable formulation. From the above results, it can be derived that clove oil loaded microemulsion based gel can be a promising alternative to current antifungal regimens.

A Study on Perception and Attitude of Students Regarding Factors Which They Consider While Making Selection of Institute in MBA Programme in Gujarat State

Abstract: Education is one among the necessary service of human beings. Now a day, MBA is one of the career choices student make to pursue their post graduation studies. The objective of study is to analyze how various factors has influence on the decision of students in selecting institute for master degree. For detail study, the questionnaire was developed and all factors which can affect institute selection decision are divided into eight categories namely, Placement related factors, extra co-curricular activities related factors, infrastructure related factors, student related factors, faculty related factors, academics related factors, advertisement related factors & other factors. Study was carried out by use of factor analysis & means score analysis. The study was undertaken with sample of 150 students. While selecting the institute, placement activities done by the institute, computer lab facility, suggestion from friends and family, career goal of the students, positive word of mouth, experience of the faculty, guidance from the counselor, specialization offered and course provided by the institute, brand name of the institute & geographic location are mostly considered by students.

Validation of Simultaneous Quantitative Method of HIV Protease Inhibitors Atazanavir, Darunavir and Ritonavir in Human Plasma by UPLC-MS/MS

Abstract: Objectives. HIV protease inhibitors are used in the treatment of patients suffering from AIDS and they act at the final stage of viral replication by interfering with the HIV protease enzyme. The paper describes a selective, sensitive, and robust method for simultaneous determination of three protease inhibitors atazanavir, darunavir and ritonavir in human plasma by ultra performance liquid chromatography-tandem mass spectrometry. Materials and Methods. The sample pretreatment consisted of solid phase extraction of analytes and their deuterated analogs as internal standards from 50 μL human plasma. Chromatographic separation of analytes was performed on Waters Acquity UPLC C18 (50 × 2.1 mm, 1.7 μm) column under gradient conditions using 10 mM ammonium formate, pH 4.0, and acetonitrile as the mobile phase. Results. The method was established over a concentration range of 5.0–6000 ng/mL for atazanavir, 5.0–5000 ng/mL for darunavir and 1.0–500 ng/mL for ritonavir. Accuracy, precision, matrix effect, recovery, and stability of the analytes were evaluated as per US FDA guidelines. Conclusions. The efficiency of sample preparation, short analysis time, and high selectivity permit simultaneous estimation of these inhibitors. The validated method can be useful in determining plasma concentration of these protease inhibitors for therapeutic drug monitoring and in high throughput clinical studies.

Formulation of curcuminoid loaded solid lipid nanoparticles in order to improve oral bioavailability

Abstract: Objective : Solid lipid nanoparticles (SLNs) of Curcuminoids were formulated and characterized in order to improve poor oral bioavailability of Curcumin. In vivo pharmacokinetics study in rats was conducted to demonstrate improved oral bioavailability. Methods : High pressure homogenization followed by ultrasonication method was adopted to formulate solid lipid nanoparticles of Curcumin. Compritol 888 ATO and Precirol ATO 5 were explored as solid lipids with LIPOID S 75 being used as surfactant. Freeze dried solid lipid nanoparticles were compared with marketed formulation of Curcumin (Adcumin®) in rat plasma using High Pressure Liquid Chromatography (HPLC) method using ultraviolet (UV) detector. Results : Particle size measurements performed on Solid lipid nanoparticles of Curcumin revealed the mean particle size of 200-300 nm for optimized formulations and entrapment efficiency of close to 80%. Sucrose and Dextrose were suitable cryoprotectants to prepare freeze dried solid lipid nanoparticles. Curcumin loaded solid lipid nanoparticles exhibited sustained release pattern during in vitro release kinetics. Conclusion : In vivo pharmacokinetics study in Swiss albino rats revealed that encapsulation of Curcumin into solid lipid nanoparticles increased oral bioavailability of Curcumin to 12 folds when compared with marketed formulation of Raw Curcumin (Adcumin®).

Rapid and sensitive UPLC-MS-MS determination of tacrolimus in Wistar rats and human blood

Abstract: A simple, sensitive and high throughput ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS-MS) method was developed for the determination of tacrolimus in the whole blood of Wistar rats and humans. Sample preparation involved protein precipitation of the analyte, using sirolimus as the internal standard with ZnSO4 from 50 µL of rat blood/human blood, followed by solid-phase extraction. Chromatographic analysis was conducted on a Waters Acquity UPLC BEH C18 column (50 × 2.1 mm, 1.7 µm) using 10 mM ammonium acetate (pH 6.0) and methanol (5:95, v/v) under isocratic conditions and detection by MS-MS. Quantitation of the analytes was achieved by multiple reaction monitoring under positive ionization mode. The method was validated over a dynamic concentration range of 0.200-200 ng/mL and had a chromatographic run time of 1.2 min. The extraction recovery for tacrolimus was >96% across three quality control levels. Matrix effect was assessed by the precision (coefficient of variation) values for the calculated slopes of calibration curves from six lots of blood. The method was applied to a pre-clinical study in 25 rats and to a bioequivalence study in 20 healthy Indian subjects. The reproducibility of the assay was successfully demonstrated by the reanalysis of 80 subject samples.