Karlsruhe Institute of Technology

About: Karlsruhe Institute of Technology is a education organization based out in Karlsruhe, Germany. It is known for research contribution in the topics: Computer science & Catalysis. The organization has 37946 authors who have published 82138 publications receiving 2197068 citations. The organization is also known as: KIT & University of Karlsruhe.

Secretome protein enrichment identifies physiological BACE1 protease substrates in neurons

Abstract: Cell surface proteolysis is essential for communication between cells and results in the shedding of membrane-protein ectodomains. However, physiological substrates of the contributing proteases are largely unknown. We developed the secretome protein enrichment with click sugars (SPECS) method, which allows proteome-wide identification of shedding substrates and secreted proteins from primary cells, even in the presence of serum proteins. SPECS combines metabolic glycan labelling and click chemistry-mediated biotinylation and distinguishes between cellular and serum proteins. SPECS identified 34, mostly novel substrates of the Alzheimer protease BACE1 in primary neurons, making BACE1 a major sheddase in the nervous system. Selected BACE1 substrates—seizure-protein 6, L1, CHL1 and contactin-2—were validated in brains of BACE1 inhibitor-treated and BACE1 knock-out mice. For some substrates, BACE1 was the major sheddase, whereas for other substrates additional proteases contributed to total substrate shedding. The new substrates point to a central function of BACE1 in neurite outgrowth and synapse formation. SPECS is also suitable for quantitative secretome analyses of primary cells and may be used for the discovery of biomarkers secreted from tumour or stem cells.

Effect of Dynamic Interfacial Tension on the Emulsification Process Using Microporous, Ceramic Membranes☆

Abstract: Conventional devices used in industrial emulsification processes disperse the inner phase by droplet disruption of high energetic laminar or turbulent flow. Membrane emulsification is different because small droplets are directly formed at the surface of a microporous membrane. Energy consumption of the process is lower, and the stresses on the system at the membrane surface and inside the pores are smaller. This allows processing of shear-sensitive substances. The result of the emulsification process can be described by the mean droplet size and the flux of the disperse phase. Among other parameters, pore size of the membrane, pressure of the disperse phase, and adsorption kinetics of the emulsifier influence the results of emulsification. The faster the emulsifier molecules adsorb at newly formed interfaces, the smaller the droplets of the emulsion produced. Transmembrane pressure greatly influences the flux but causes little change in droplet size.

Size-Dependent Optical Spectroscopy of a Homologous Series of CdSe Cluster Molecules

Abstract: The optical properties and electronic structure of a homologous series of CdSe cluster molecules covering a size range between 0.7 and 2 nm are investigated. CdSe cluster molecules with 4, 8 10, 17, and 32 Cd atoms, capped by selenophenol ligands, were crystallized from solution and their structures determined by single-crystal X-ray diffraction. The cluster molecules are composed of a combination of adamanthane and barylene-like cages, the building blocks of the zinc blende and the wurtzite structures of the bulk CdSe. The onset of the room temperature absorption and low-temperature photoluminescence excitation spectra exhibit a systematic blue shift with reduced cluster size manifesting the quantum confinement effect down to the molecular limit of the bulk semiconductor. Blue-green emission, shifted substantially to lower energy from the absorption onset, is observed only at low temperature and its position is nearly independent of cluster size. The wavelength dependence of both photoluminescence and ph...

Evidence for a particle produced in association with weak bosons and decaying to a bottom-antibottom quark pair in higgs boson searches at the tevatron

Abstract: We combine searches by the CDF and D0 Collaborations for the associated production of a Higgs boson with a W or Z boson and subsequent decay of the Higgs boson to a bottom-antibottom quark pair. The data, originating from Fermilab Tevatron p (p) over bar collisions at root s = 1.96 TeV, correspond to integrated luminosities of up to 9.7 fb(-1). The searches are conducted for a Higgs boson with mass in the range 100-150 GeV/c(2). We observe an excess of events in the data compared with the background predictions, which is most significant in the mass range between 120 and 135 GeV/c(2). The largest local significance is 3.3 standard deviations, corresponding to a global significance of 3.1 standard deviations. We interpret this as evidence for the presence of a new particle consistent with the standard model Higgs boson, which is produced in association with a weak vector boson and decays to a bottom-antibottom quark pair.

Strong spin-phonon coupling between a single-molecule magnet and a carbon nanotube nanoelectromechanical system

Abstract: The coupling between a single-molecule spin and a single phonon in a carbon nanotube is observed.