Swedish Defence Research Agency

About: Swedish Defence Research Agency is a government organization based out in Stockholm, Sweden. It is known for research contribution in the topics: Radar & Laser. The organization has 1413 authors who have published 2731 publications receiving 56083 citations. The organization is also known as: Totalförsvarets forskningsinstitut.

Laser scanning of forest resources: the nordic experience

Abstract: This article reviews the research and application of airborne laser scanning for forest inventory in Finland, Norway and Sweden. The first experiments with scanning lasers for forest inventory were conducted in 1991 using the FLASH system, a full-waveform experimental laser developed by the Swedish Defence Research Institute. In Finland at the same time, the HUTSCAT profiling radar provided experiences that inspired the following laser scanning research. Since 1995, data from commercially operated time-of-flight scanning lasers (e.g. TopEye, Optech ALTM and TopoSys) have been used. Especially in Norway, the main objective has been to develop methods that are directly suited for practical forest inventory at the stand level. Mean tree height, stand volume and basal area have been the most important forest mensurational parameters of interest. Laser data have been related to field training plot measurements using regression techniques, and these relationships have been used to predict corresponding properti...

The complete genome sequence of Francisella tularensis, the causative agent of tularemia.

Abstract: Francisella tularensis is one of the most infectious human pathogens known. In the past, both the former Soviet Union and the US had programs to develop weapons containing the bacterium. We report the complete genome sequence of a highly virulent isolate of F. tularensis (1,892,819 bp). The sequence uncovers previously uncharacterized genes encoding type IV pili, a surface polysaccharide and iron-acquisition systems. Several virulence-associated genes were located in a putative pathogenicity island, which was duplicated in the genome. More than 10% of the putative coding sequences contained insertion-deletion or substitution mutations and seemed to be deteriorating. The genome is rich in IS elements, including IS630 Tc-1 mariner family transposons, which are not expected in a prokaryote. We used a computational method for predicting metabolic pathways and found an unexpectedly high proportion of disrupted pathways, explaining the fastidious nutritional requirements of the bacterium. The loss of biosynthetic pathways indicates that F. tularensis is an obligate host-dependent bacterium in its natural life cycle. Our results have implications for our understanding of how highly virulent human pathogens evolve and will expedite strategies to combat them.

Survival and Growth of Francisella tularensis in Acanthamoeba castellanii

Abstract: Francisella tularensis is a highly infectious, facultative intracellular bacterium which causes epidemics of tularemia in both humans and mammals at regular intervals. The natural reservoir of the bacterium is largely unknown, although it has been speculated that protozoa may harbor it. To test this hypothesis, Acanthamoeba castellanii was cocultured with a strain of F. tularensis engineered to produce green fluorescent protein (GFP) in a nutrient-rich medium. GFP fluorescence within A. castellanii was then monitored by flow cytometry and fluorescence microscopy. In addition, extracellular bacteria were distinguished from intracellular bacteria by targeting with monoclonal antibodies. Electron microscopy was used to determine the intracellular location of F. tularensis in A. castellanii, and viable counts were obtained for both extracellular and intracellular bacteria. The results showed that many F. tularensis cells were located intracellularly in A. castellanii cells. The bacteria multiplied within intracellular vacuoles and eventually killed many of the host cells. F. tularensis was found in intact trophozoites, excreted vesicles, and cysts. Furthermore, F. tularensis grew faster in cocultures with A. castellanii than it did when grown alone in the same medium. This increase in growth was accompanied by a decrease in the number of A. castellanii cells. The interaction between F. tularensis and amoebae demonstrated in this study indicates that ubiquitous protozoa might be an important environmental reservoir for F. tularensis.