Showing papers in "Trends in Biotechnology in 2019"

TL;DR: A detailed classification of CRISPR/Cas biosensing systems is provided and they have the potential to become promising candidates for next-generation diagnostic biosensing platforms.

TL;DR: This review aims to provide a focused overview of the latest achievements made in MIP-based sensor technology, with emphasis on research toward real-life applications.

TL;DR: The critical pitfalls and sources of error in qPCR experiments are described, along with a rigorous, stepwise process to minimize variability, time, and cost in generating reproducible, publication quality data every time.

TL;DR: The monoclonal antibody (mAb) market has changed rapidly in the past 5 years: it has doubled in size, becoming dominated by fully human molecules, launched bispecific molecules, and faced competition from biosimilars.

TL;DR: Current trends in systems metabolic engineering including tools and strategies are reviewed, focusing on recent developments in selection of host strains, metabolic pathway reconstruction, tolerance enhancement, and metabolic flux optimization.

TL;DR: Recent advancements in synthetic biology tools and approaches to engineer synthetic microbial Consortia are reviewed, ongoing and emerging efforts to apply consortia for various biotechnological applications are discussed, and future applications are suggested.

TL;DR: The development of various base editors are reviewed, their technical advantages and limitations are assessed, and their broad applications in basic research, medicine, and agriculture are discussed.

TL;DR: Deep eutectic solvents (DESs) have recently been evaluated assolvents in different biocatalytic reactions and can improve substrate supply, conversion, and stability.

TL;DR: Emerging state-of-the-art techniques that customize algae for enhanced performance and selectivity, particularly molecular and chemical extraction techniques as well as nanoparticle (NP) synthesis approaches are emphasized.

TL;DR: A review of zinc-based degradable biomaterials highlights recent developments, discusses obstacles to overcome, and pinpoints directions for future research.

TL;DR: The mechanism of biofilm formation is summarized, current diagnostic approaches of chronic wound biofilms based on morphology, microbiology, and molecular assays are focused on, and innovative biotechnological approaches may further shed light on this unmet clinical need.

TL;DR: Cytochromes P450 (P450 or CYP) are heme-containing enzymes that catalyze the introduction of one atom of molecular oxygen into nonactivated C-H bonds, often in a regio- and stereoselective manner, which makes P450s powerful biocatalysts.

TL;DR: New perspectives and challenges are identified in protein properties and in the integration and cooptimization of culture and downstream processes and promising solutions are proposed for realizing microalgal biorefineries at industrial scale.

TL;DR: This review addresses lignin and its role in the hydrolysis of hardwood and other lignocellulosic residues and the cost of enzymes.

TL;DR: Understanding the role of crosslinking agents in the modification of collagen performance and their potential biomedical applications are crucial for developing novel collagen-based biopolymers for therapeutic gain.

TL;DR: A holistic temporal model of the shifting constraints on inoculants at five stages of product development and application is proposed, and potential conflicts between stages are highlighted.

TL;DR: A roadmap to integrating deep learning and microfluidics in biotechnology laboratories that matches computational architectures to problem types, and provides an outlook on emerging opportunities is provided.

TL;DR: How single-molecule sequencing technologies are opening up new clinical avenues that are being applied to pathogenic microorganisms and viruses, constitutional disorders, pharmacogenomics, cancer, and more is reviewed.

TL;DR: The most recent advances in BBB-on-chip devices are presented and validation steps that will strengthen their future applications are examined, including modeling of neurodegenerative diseases and high-throughput drug screening.

TL;DR: The fabrication of exosome detection biosensors with respect to biological recognition strategies and signal transduction techniques, which involve integrated scientific and technological aspects of analytical chemistry and nanotechnology are described.

TL;DR: The roles of biotechnology and cell therapy industries to manufacture MVs as inherent therapeutic agents or drug delivery systems are summarized and the manufacturing, development, characterization, and regulatory challenges for successful translation are discussed.

TL;DR: The challenges and future opportunities of this nascent industry are addressed by elucidating key elements of the biorefining sector, including feedstock sourcing, processing methods, and the bioproducts market.

TL;DR: The current state of genomics and field phenomics is presented, emerging approaches and challenges for multiomics big data integration by means of next-generation (Next-Gen) artificial intelligence (AI) are explored, and a workable path to improvement is proposed.

TL;DR: The most recent biological parts and approaches developed are covered and the missing tools needed to build robust living therapeutics are described.

TL;DR: A critical assessment of the various RNAi/CRISPR approaches to pooled screens to discuss their advantages and pitfalls and provides a detailed overview of analysis methods and repositories for identifying the best candidate gene hits.

TL;DR: Zymomonas mobilis not only can be engineered for pentose metabolism through the isomerase pathway without cofactor imbalance but also metabolizes sugar through the Entner-Doudoroff pathway with less ATP and biomass produced for more sugar to be used for ethanol production.

TL;DR: Using these organisms, and ambitions towards zero-concepts with respect to emissions and excess resources in bioprocess engineering, industrial biotechnology is expected to become highly integrated into sustainable generations of technology systems.

TL;DR: The current scientific and regulatory landscape surrounding the implementation of integrated continuous biomanufacturing is examined, which requires the uninterrupted connection of continuous unit operations with no isolated intermediate or hold steps occurring between them.

TL;DR: This review summarizes current understanding of the interplay between the curvature of a cell substrate and the related morphological and functional cellular response and highlights state-of-the-art microtechnologies for engineering curved biomaterials at cell-scale.

TL;DR: This review focuses on recent advancements and modern approaches in quinolone sensors and biosensors that are promising future platforms for rapid and on-site monitoring of antibiotic residues.