Journal ArticleDOI
CRISPR/Cas Systems towards Next-Generation Biosensing.
TLDR
A detailed classification of CRISPR/Cas biosensing systems is provided and they have the potential to become promising candidates for next-generation diagnostic biosensing platforms.About:
This article is published in Trends in Biotechnology.The article was published on 2019-07-01. It has received 518 citations till now. The article focuses on the topics: Cas9 & CRISPR.read more
Citations
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Journal ArticleDOI
SHERLOCK: nucleic acid detection with CRISPR nucleases.
Max J. Kellner,Jeremy G. Koob,Jonathan S. Gootenberg,Jonathan S. Gootenberg,Jonathan S. Gootenberg,Omar O. Abudayyeh,Omar O. Abudayyeh,Omar O. Abudayyeh,Feng Zhang +8 more
TL;DR: Step-by-step instructions for setting up SHERLOCK assays with recombinase-mediated polymerase pre-amplification of DNA or RNA and subsequent Cas13- or Cas12-mediated detection via fluorescence and colorimetric readouts that provide results in <1 h with a setup time of less than 15 min are provided.
Journal ArticleDOI
Ultrasensitive and visual detection of SARS-CoV-2 using all-in-one dual CRISPR-Cas12a assay.
Xiong Ding,Kun Yin,Ziyue Li,Rajesh V. Lalla,Enrique Ballesteros,Maroun M. Sfeir,Changchun Liu +6 more
TL;DR: The AIOD-CRISPR method has the significant potential to provide a rapid, sensitive, one-pot point-of-care assay for SARS-CoV-2.
Journal ArticleDOI
Diagnostics for SARS-CoV-2 infections.
Bhavesh D. Kevadiya,Jatin Machhi,Jonathan Herskovitz,Maxim D. Oleynikov,Wilson R. Blomberg,Neha Bajwa,Dhruvkumar Soni,Srijanee Das,Mahmudul Hasan,Milankumar Patel,Ahmed M. Senan,Santhi Gorantla,JoEllyn M McMillan,Benson J Edagwa,Robert Eisenberg,Channabasavaiah B. Gurumurthy,St Patrick Reid,Chamindie Punyadeera,Linda Chang,Howard E. Gendelman +19 more
TL;DR: A review of available and in-development diagnostic tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) including nanomaterial-based tools is presented in this paper.
Journal ArticleDOI
Exploring the Trans-Cleavage Activity of CRISPR-Cas12a (cpf1) for the Development of a Universal Electrochemical Biosensor.
TL;DR: The first CRISPR Cas12a (cpf1) based electrochemical biosensor (E-CRISPR) is reported, which is more cost-effective and portable comparing with optical transduction based biosensing systems and could be a powerful enabler for wide developments of portable, accurate, and cost-efficient point-of-care diagnostic systems.
Journal ArticleDOI
Ultra-sensitive and high-throughput CRISPR-p owered COVID-19 diagnosis.
Zhen Huang,Di Tian,Yang Liu,Zhen Lin,Christopher J. Lyon,Weihua Lai,Dahlene N. Fusco,Arnaud Drouin,Xiao-Ming Yin,Tony Y. Hu,Bo Ning +10 more
TL;DR: This assay utilizes a custom CRISPR Cas12a/gRNA complex and a fluorescent probe to amplify target amplicons produced by standard RT-PCR or isothermal recombinase polymerase amplification (RPA) to allow sensitive detection at sites not equipped with real-time PCR systems required for qPCR diagnostics.
References
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Journal ArticleDOI
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
Journal ArticleDOI
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
TL;DR: The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage as discussed by the authors.
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,F. A. Ran,David Benjamin Turitz Cox,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +10 more
TL;DR: Two different type II CRISPR/Cas systems are engineered and it is demonstrated that Cas9 nucleases can be directed by short RNAs to induce precise cleavage at endogenous genomic loci in human and mouse cells, demonstrating easy programmability and wide applicability of the RNA-guided nuclease technology.
Journal ArticleDOI
RNA-Guided Human Genome Engineering via Cas9
Prashant Mali,Luhan Yang,Kevin M. Esvelt,John Aach,Marc Güell,James E. DiCarlo,Julie E. Norville,George M. Church,George M. Church +8 more
TL;DR: The type II bacterial CRISPR system is engineer to function with custom guide RNA (gRNA) in human cells to establish an RNA-guided editing tool for facile, robust, and multiplexable human genome engineering.
Journal ArticleDOI
Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system.
Bernd Zetsche,Jonathan S. Gootenberg,Omar O. Abudayyeh,Ian Slaymaker,Kira S. Makarova,Patrick Essletzbichler,Sara E. Volz,Julia Joung,John van der Oost,Aviv Regev,Aviv Regev,Eugene V. Koonin,Feng Zhang +12 more
TL;DR: In this paper, the authors characterized Cpf1, a putative class 2 CRISPR effector, which is a single RNA-guided endonuclease lacking tracrRNA and utilizes a T-rich protospacer-adjacent motif.
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