A genetically encoded anti-CRISPR protein constrains gene drive spread and prevents population suppression.
Chrysanthi Taxiarchi,Andrea Beaghton,Nayomi Illansinhage Don,Kyros Kyrou,Matthew Gribble,Dammy Shittu,Scott P. Collins,Chase L. Beisel,Chase L. Beisel,Roberto Galizi,Andrea Crisanti,Andrea Crisanti +11 more
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TLDR
In this article, an approach based on the germline expression of a phage-derived anti-CRISPR protein (AcrIIA4) was proposed to inactivate CRISPR-based gene drives and restore their inheritance to Mendelian rates in the malaria vector Anopheles gambiae.Abstract:
CRISPR-based gene drives offer promising means to reduce the burden of pests and vector-borne diseases. These techniques consist of releasing genetically modified organisms carrying CRISPR-Cas nucleases designed to bias their inheritance and rapidly propagate desired modifications. Gene drives can be intended to reduce reproductive capacity of harmful insects or spread anti-pathogen effectors through wild populations, even when these confer fitness disadvantages. Technologies capable of halting the spread of gene drives may prove highly valuable in controlling, counteracting, and even reverting their effect on individual organisms as well as entire populations. Here we show engineering and testing of a genetic approach, based on the germline expression of a phage-derived anti-CRISPR protein (AcrIIA4), able to inactivate CRISPR-based gene drives and restore their inheritance to Mendelian rates in the malaria vector Anopheles gambiae. Modeling predictions and cage testing show that a single release of male mosquitoes carrying the AcrIIA4 protein can block the spread of a highly effective suppressive gene drive preventing population collapse of caged malaria mosquitoes.read more
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Combating mosquito-borne diseases using genetic control technologies.
Guan-Hong Wang,Guan-Hong Wang,Stephanie Gamez,Robyn R. Raban,John M. Marshall,Luke Alphey,Ming Li,Jason L. Rasgon,Omar S. Akbari +8 more
TL;DR: A review of the latest developments, notable similarities, and critical distinctions between these promising technologies and discuss their future applications for mosquito-borne disease control can be found in this paper, where the authors discuss the future applications of these technologies.
Journal ArticleDOI
Gene drives gaining speed
TL;DR: The authors summarizes the progress in this field, focusing on optimal design features for full-drive elements that either suppress target mosquito populations or modify them to prevent pathogen transmission, allelic drives for updating genetic elements, mitigating strategies including trans-complementing split-drives and genetic neutralizing elements, and the adaptation of drive technology to other organisms.
Journal ArticleDOI
Symbionts and gene drive: two strategies to combat vector-borne disease.
TL;DR: In this paper , Bhatt et al. reviewed the latest developments in both symbionts and gene drive-based methods, as well as distinctions and obstacles relating to these promising technologies.
Journal ArticleDOI
A homing suppression gene drive with multiplexed gRNAs maintains high drive conversion efficiency and avoids functional resistance alleles
TL;DR: In this paper , the authors constructed a homing suppression gene drive in Drosophila melanogaster that utilized multiplexed gRNAs to inhibit the formation of functional resistance alleles in its female fertility target gene.
Journal ArticleDOI
Risk management recommendations for environmental releases of gene drive modified insects.
Yann Devos,Yann Devos,John D. Mumford,Michael B. Bonsall,Debora C.M. Glandorf,Hector Quemada +5 more
TL;DR: In this article, the authors provide recommendations that may help to improve the relevance of risk assessment and risk management frameworks for environmental releases of gene drive modified insects (GDMIs) by developing additional and more practical risk assessment guidance to ensure appropriate levels of safety.
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