A H-bond strategy to develop acid-resistant photoswitchable rhodamine spirolactams for super-resolution single-molecule localization microscopy
Qingkai Qi,Weijie Chi,Yuanyuan Li,Qinglong Qiao,Jie Chen,Lu Miao,Yi Zhang,Jin Li,Wei Ji,Tao Xu,Xiaogang Liu,Juyoung Yoon,Zhaochao Xu +12 more
TLDR
In this article, a series of acid-resistant rhodamine spirolactams with accelerated ring-closing reactions from fluorescent xanthyliums to non-fluorescent spiactams, endowing them with good photoswitchable properties even in acidic environments.Abstract:
Rhodamine spirolactam based photoswitches have been extensively applied in super-resolution single-molecule localization microscopy (SMLM). However, the ring-opening reactions of spirolactams are cross-sensitive to acid, limiting their photoswitch use to neutral pH conditions. In addition, the ring-closing reactions of spirolactams are environment-sensitive and slow (up to hours), virtually making rhodamine spirolactams caged fluorescent dyes instead of reversible photoswitches in SMLM. Herein, by introducing hydrogen bonds to stabilize spirolactams, we report a series of acid-resistant rhodamine spirolactams with accelerated ring-closing reactions from fluorescent xanthyliums to non-fluorescent spirolactams, endowing them with good photoswitchable properties even in acidic environments. By further substitution of 6-phenylethynyl naphthalimide on the spirolactam, we shifted the photoactivation wavelength into the visible region (>400 nm). Subsequently, we have successfully applied these dyes in labeling and imaging the cell surface of Bacillus subtilis at pH 4.5 using SMLM.read more
Citations
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Metal-coordinated fluorescent and luminescent probes for reactive oxygen species (ROS) and reactive nitrogen species (RNS)
TL;DR: In this paper, a review of reactive oxygen species (ROS)-selective fluorescent and luminescent probes based on metal-coordinated systems is presented, where metal ions play key roles in the probes as an on-off redox switch for photoinduced quenching and as a reaction site with ROS, RNS or luminecenters.
Journal ArticleDOI
Quaternary Piperazine-Substituted Rhodamines with Enhanced Brightness for Super-Resolution Imaging.
Zhiwei Ye,Wei Yang,Chao Wang,Ying Zheng,Weijie Chi,Xiaogang Liu,Zhenlong Huang,Xiaoyuan Li,Yi Xiao +8 more
TL;DR: A new class of quaternary piperazine substituted rhodamines with outstanding quantum yields and superior brightness is developed, by utilizing the electronic inductive effect to prevent TICT.
Journal ArticleDOI
A General Descriptor ΔE Enables the Quantitative Development of Luminescent Materials Based on Photoinduced Electron Transfer
Weijie Chi,Jie Chen,Wenjuan Liu,Chao Wang,Chao Wang,Qingkai Qi,Qinglong Qiao,Tee Meng Tan,Kangming Xiong,Xiao Liu,Keegan Kang,Young-Tae Chang,Zhaochao Xu,Xiaogang Liu +13 more
TL;DR: This work developed a general descriptor (ΔE) for predicting the quantum yield of PET probes, with a threshold value of ~0.6 eV, that is applicable to a wide range of fluorophores, such as BODIPY, fluorescein, rhodamine, and Si-Rhodamine.
Journal ArticleDOI
Rhodamine B Adsorptive Removal and Photocatalytic Degradation on MIL-53-Fe MOF/Magnetic Magnetite/Biochar Composites
Chanaka M. Navarathna,Narada Bombuwala Dewage,Akila G. Karunanayake,Erin L. Farmer,Felio Perez,El Barbary Hassan,Todd E. Mlsna,Charles U. Pittman +7 more
TL;DR: In this paper, a triphase hybrid, multifunctional, magnetically recoverable, sorptive, photocatalytic and degradative, adsorbent (MOF-MBC) was used both to remove and catalyze the photodegradation of Rhodamine B (Rh B) with or without Cr6+ present.
Journal ArticleDOI
A new mitochondrion targetable fluorescent probe for carbon monoxide-specific detection and live cell imaging
Chenxia Zhang,Hai Xie,Tongxia Zhan,Jie Zhang,Bochao Chen,Zhaosheng Qian,Guanglu Zhang,Weifen Zhang,Jin Zhou +8 more
TL;DR: A new mitochondrion targetable molecular probe for carbon monoxide (CO)-specific detection based on palladium-free mediated opening of spirolactam was designed and enables a safe and powerful method for unravelling the function of CO in biological systems to be established.
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