A high-throughput chemically induced inflammation assay in zebrafish
Claudia A d'Alençon,Oscar A Peña,Christine Wittmann,Viviana E Gallardo,Rebecca A Jones,Felix Loosli,Urban Liebel,Clemens Grabher,Miguel L. Allende +8 more
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TLDR
It is shown that specific, noninvasive damage to lateral line neuromast cells can induce a robust acute inflammatory response and be transformed into a high-throughput screening method by using a customized automated imaging and processing system that quantifies the magnitude of the inflammatory reaction.Abstract:
Background
Studies on innate immunity have benefited from the introduction of zebrafish as a model system. Transgenic fish expressing fluorescent proteins in leukocyte populations allow direct, quantitative visualization of an inflammatory response in vivo. It has been proposed that this animal model can be used for high-throughput screens aimed at the identification of novel immunomodulatory lead compounds. However, current assays require invasive manipulation of fish individually, thus preventing high-content screening.read more
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Combinatorial Strategies in Fluorescent Probe Development
TL;DR: This poster presents a poster presented at the 2016 Singapore International Conference of the American Association for the Advancement of Science and Technology (A*STAR) entitled “Advances in Bioimaging Probe Development: Foundations of a scalable, scalable and reproducible Nanofiltration Probe for Matrix-based Assays.”
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A model 450 million years in the making: zebrafish and vertebrate immunity.
TL;DR: Zebrafish immunity is reviewed with a particular focus on recent studies that exploit the unique genetic and in vivo imaging advantages available for this organism, which are driving forward the study of vertebrate immunity in general.
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A Zebrafish Compound Screen Reveals Modulation of Neutrophil Reverse Migration as an Anti-Inflammatory Mechanism
Anne L. Robertson,Geoffrey Holmes,Aleksandra Bojarczuk,Joseph Burgon,Catherine A. Loynes,Myriam Chimen,Amy Sawtell,Bashar Hamza,Joseph A Willson,Sarah R. Walmsley,Sean R. Anderson,Mark Coles,Stuart N. Farrow,Roberto Solari,Simon Jones,Lynne R. Prince,Daniel Irimia,G. Ed Rainger,Visakan Kadirkamanathan,Moira K. B. Whyte,Stephen A. Renshaw +20 more
TL;DR: The authors used a transgenic zebrafish model of sterile tissue injury to screen potential factors involved in inflammation resolution and found that tanshinone IIA, which is derived from a Chinese medicinal herb, had proresolving activity by both inducing neutrophil apoptosis and promoting reverse migration of neutrophils.
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Sustainable aquaculture requires environmental-friendly treatment strategies for fish diseases
Thora Lieke,Thora Lieke,Thomas Meinelt,Seyed Hossein Hoseinifar,Bo Pan,David L. Straus,Christian E. W. Steinberg,Christian E. W. Steinberg +7 more
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Copper toxicology, oxidative stress and inflammation using zebrafish as experimental model
TL;DR: An overview of copper metabolism in health and disease and its effects on oxidative stress and inflammation responses in zebrafish models is provided, and copper‐induced inflammation is highlighted owing to its potential to easily mimic pro‐oxidative and pro‐inflammatory features that combined with zebra fish genetic tractability could help further in the understanding of Copper metabolism, inflammatory responses and related diseases.
References
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Journal ArticleDOI
Stages of embryonic development of the zebrafish.
TL;DR: A series of stages for development of the embryo of the zebrafish, Danio (Brachydanio) rerio is described, providing for flexibility and continued evolution of the staging series as the authors learn more about development in this species.
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Cancer Chemopreventive Activity of Resveratrol, a Natural Product Derived from Grapes
Mei-Shiang Jang,Lining Cai,George Udeani,Karla Slowing,Cathy F. Thomas,Chris Beecher,Harry H. S. Fong,Norman R. Farnsworth,A. Douglas Kinghorn,Rajendra G. Mehta,Richard C. Moon,John M. Pezzuto +11 more
TL;DR: It is suggested that resveratrol, a common constituent of the human diet, merits investigation as a potential cancer chemopreventive agent in humans.
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The identification of genes with unique and essential functions in the development of the zebrafish, Danio rerio
P. Haffter,Michael Granato,Michael Brand,Mary C. Mullins,M. Hammerschmidt,D. A. Kane,J. Odenthal,F. J. M. Van Eeden,Y.-J. Jiang,Carl-Philipp Heisenberg,Robert N. Kelsh,Makoto Furutani-Seiki,Elisabeth Vogelsang,D. Beuchle,U. Schach,C. Fabian,Christiane Nüsslein-Volhard +16 more
TL;DR: It is estimated that the 372 genes defined by the mutants probably represent more than half of all genes that could have been discovered using the criteria of the screen, and the limits and the potentials of a genetic saturation screen in the zebrafish.
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A tissue-scale gradient of hydrogen peroxide mediates rapid wound detection in zebrafish
TL;DR: This work reveals a sustained rise in H2O2 concentration at the wound margin, and shows that this gradient is created by dual oxidase (Duox), and that it is required for rapid recruitment of leukocytes to the wound.