Journal ArticleDOI
A mouse model for β-Thalassemia
Loren C. Skow,B.A. Burkhart,F. M. Johnson,Raymond A. Popp,Diana M. Popp,SZ Goldberg,WF Anderson,L. B. Barnett,Susan E. Lewis +8 more
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TLDR
Mice homozygous for the mutant allele, designated Hbb th-1 , represent the first animal model of β-thalassemia (Cooley's anemia), a severe genetic disease of humans.About:
This article is published in Cell.The article was published on 1983-10-01. It has received 200 citations till now. The article focuses on the topics: Microcytic anemia & Reticulocytosis.read more
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Journal ArticleDOI
Epigenetic memory in induced pluripotent stem cells
Kibem Kim,Akiko Doi,Bo Wen,K. Ng,Rui Zhao,Patrick Cahan,Jonghwan Kim,Martin J. Aryee,Hong Ji,Lauren I.R. Ehrlich,Lauren I.R. Ehrlich,A. Yabuuchi,A. Takeuchi,K. C. Cunniff,H. Hongguang,Shannon McKinney-Freeman,Olaia Naveiras,T. J. Yoon,T. J. Yoon,Rafael A. Irizarry,Namyoung Jung,Jun Seita,Jacob H. Hanna,Peter Murakami,Rudolf Jaenisch,Ralph Weissleder,Stuart H. Orkin,Irving L. Weissman,Andrew P. Feinberg,George Q. Daley +29 more
TL;DR: It is observed that low-passage induced pluripotent stem cells (iPSCs) derived by factor-based reprogramming of adult murine tissues harbour residual DNA methylation signatures characteristic of their somatic tissue of origin, which favours their differentiation along lineages related to the donor cell, while restricting alternative cell fates.
Epigenetic memory in induced pluripotent stem cells
Kibem Kim,Akiko Doi,Bo Wen,K. Ng,Rui Zhao,Patrick Cahan,Jonghwan Kim,Martin J. Aryee,Hong Ji,Lauren I.R. Ehrlich,Lauren I.R. Ehrlich,A. Yabuuchi,A. Takeuchi,K. C. Cunniff,H. Hongguang,Shannon McKinney-Freeman,Olaia Naveiras,T. J. Yoon,T. J. Yoon,Rafael A. Irizarry,Namyoung Jung,Jun Seita,Jacob H. Hanna,Peter Murakami,Rudolf Jaenisch,Ralph Weissleder,Stuart H. Orkin,Irving L. Weissman,Andrew P. Feinberg,George Q. Daley +29 more
TL;DR: The authors showed that low-passage induced pluripotent stem cells (iPSCs) derived by factor-based reprogramming of adult murine tissues harbor residual DNA methylation signatures characteristic of their somatic tissue of origin.
Journal ArticleDOI
Prospects for human gene therapy
TL;DR: The most effective delivery system at present uses retroviral-based vectors to transfer a gene into murine bone marrow cells in culture as discussed by the authors, and the genetically altered bone marrow is then implanted into recipient animals.
Book ChapterDOI
The molecular genetics of human hemoglobin.
TL;DR: The recognition of linked polymorphisms, the development of mutation-specific oligonucleotide probes, and the possibility of first-trimester chrionic villus biopsy have made the prenatal diagnosis of sickle-cell anemia and β-thalassemias of immediate applicability, and appropriate resources must be marshaled to offer this technology to the areas of the world where it is most needed.
Journal ArticleDOI
Targeted disruption of the apolipoprotein C-III gene in mice results in hypotriglyceridemia and protection from postprandial hypertriglyceridemia.
TL;DR: Data indicate that ApoC-III modulates the catabolism of triglyceride-rich lipoproteins and plays a role in the postprandial management of triglycerides.
References
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Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
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Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I
TL;DR: Labeled DNAs (and restriction endonuclease fragments derived from them) are useful probes for detecting rare homologous sequences by in situ hybridization and reassociation kinetic analysis.
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Abnormal human haemoglobins. Separation and characterization of the alpha and beta chains by chromatography, and the determination of two new variants, hb Chesapeak and hb J (Bangkok).
TL;DR: In this article, a method for the separation of the α and β chains of human haemoglobin by chromatography on carboxymethyl-cellulose in 8 M -urea has been described.
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A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells.
TL;DR: The covalently closed circular duplex DNA structure, originally identified in polyoma viral DNA,1’2 has been assigned to the mitochondrial DNA’s in ox and sheep heart, in mouse and chicken liver, and in unfertilized sea urchin egg.
Journal ArticleDOI
The Isolation and Characterization of Linked δ- and β-Globin Genes from a Cloned Library of Human DNA
TL;DR: The two independently isolated β-globin clones differ from each other by the presence of a Pst I restriction enzyme cleavage site within the large intervening sequence of the δ- globin gene of one of the clones, which suggests that the human DNA carried in the two clones was derived from two homologous chromosomes which were heterozygous for the PstI restriction enzyme recognition sequence.