Journal ArticleDOI
Accurate determination of local defocus and specimen tilt in electron microscopy
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TLDR
Two computer programs are presented, CTFFIND3 and CTFTILT, which determine defocus parameters from images of untilted specimens, as well as defocus and tilt parameters from image of tilted specimens, respectively, using a simple algorithm.About:
This article is published in Journal of Structural Biology.The article was published on 2003-06-01. It has received 1480 citations till now. The article focuses on the topics: Tilt (optics) & Contrast transfer function.read more
Citations
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Robo1 Forms a Compact Dimer-of-Dimers Assembly
Nataliia Aleksandrova,Irina Gutsche,Eaazhisai Kandiah,Sergiy V. Avilov,Maxim V. Petoukhov,Elena Seiradake,Andrew A. McCarthy +6 more
TL;DR: It is proposed that Slit2-N binding results in a conformational change of Robo1 to trigger cell signaling, and is proposed to be an attractive therapeutic target for roundabout receptors.
Journal ArticleDOI
Atomic structure of the Campylobacter jejuni flagellar filament reveals how ε Proteobacteria escaped Toll-like receptor 5 surveillance
TL;DR: The essential role of glycosylation in C. jejuni motility is explained, and how the outer domains have evolved to play a role not previously found in other bacteria is shown.
Journal ArticleDOI
The structural basis for Z α1-antitrypsin polymerization in the liver
Sarah V. Faull,Emma L. K. Elliston,Bibek Gooptu,Bibek Gooptu,Bibek Gooptu,Alistair M. Jagger,Ibrahim Aldobiyan,Adam Redzej,Magd Badaoui,Nina Heyer-Chauhan,S. Tamir Rashid,Gary M. Reynolds,David H. Adams,Elena Miranda,Elena V. Orlova,James A. Irving,David A. Lomas +16 more
TL;DR: The data show structural equivalence between heat-induced and ex vivo polymers and that the intersubunit linkage is best explained by a carboxyl-terminal domain swap between molecules of α1-antitrypsin.
ComponentDOI
Seneca Valley virus attachment and uncoating mediated by its receptor anthrax toxin receptor 1
L. Cao,Ruifeng Zhang,T. Liu,Zhong Sun,M. Hu,Yunpeng Sun,Liang Cheng,Yu Guo,S. Fu,J. Hu,X. Li,Caiting Yu,H. Wang,Huilan Chen,Elizabeth E. Fry,David I. Stuart,Ping Qian,Zhiyong Lou,Zihe Rao,Zihe Rao +19 more
TL;DR: Atomic structures of mature SVV particles alone and in complex with ANTXR1 in both neutral and acidic conditions are determined, as well as empty “spent” particles in complex in acidic conditions by cryoelectron microscopy, highlighting opportunities for anticancer therapeutic optimization.
Journal ArticleDOI
Cargo Retention inside P22 Virus-Like Particles
Kimberly McCoy,Ekaterina Selivanovitch,Daniel Luque,Byeongdu Lee,Ethan Edwards,José R. Castón,Trevor Douglas +6 more
TL;DR: The factors important to the ability of P22 VLP to retain or release various protein cargo molecules depending on the nature of the cargo, the capsid morphology, and the environmental conditions are studied.
References
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Journal ArticleDOI
Three-dimensional model of purple membrane obtained by electron microscopy
Richard Henderson,P. N. T. Unwin +1 more
TL;DR: A 7-Å resolution map of the purple membrane has been obtained by electron microscopy of tilted, unstained specimens and shows that Lipid bilayer regions fill the spaces between the protein molecules.
Journal ArticleDOI
Structure of the alpha beta tubulin dimer by electron crystallography.
TL;DR: An atomic model of the αβ tubulin dimer fitted to a 3.7-Å density map obtained by electron crystallography of zinc-induced tubulin sheets is presented.
Journal ArticleDOI
Structural determinants of water permeation through aquaporin-1.
Kazuyoshi Murata,Kaoru Mitsuoka,Teruhisa Hirai,Thomas Walz,Peter Agre,J. B. Heymann,Andreas Engel,Yoshinori Fujiyoshi +7 more
TL;DR: An atomic model of human red cell AQP1 is described, providing a possible molecular explanation to a longstanding puzzle in physiology—how membranes can be freely permeable to water but impermeable to protons.
Journal ArticleDOI
Structure of dengue virus: implications for flavivirus organization, maturation, and fusion.
Richard J. Kuhn,Wei Zhang,Michael G. Rossmann,Sergei V. Pletnev,Jeroen Corver,Edith M. Lenches,Christopher T. Jones,Suchetana Mukhopadhyay,Paul R. Chipman,Ellen G. Strauss,Timothy S. Baker,James H. Strauss +11 more
TL;DR: The first structure of a flavivirus has been determined by using a combination of cryoelectron microscopy and fitting of the known structure of glycoprotein E into the electron density map, suggesting that flaviviruses employ a fusion mechanism in which the distal beta barrels of domain II of the glycop Protein E are inserted into the cellular membrane.
Journal ArticleDOI
A ratchet-like inter-subunit reorganization of the ribosome during translocation.
TL;DR: Three-dimensional cryo-electron microscopy maps of the Escherichia coli 70S ribosome in various functional states show that both EF-G binding and subsequent GTP hydrolysis lead to ratchet-like rotations of the small 30S sub unit relative to the large 50S subunit, indicating a two-step mechanism of translocation.