Journal ArticleDOI
Accurate determination of local defocus and specimen tilt in electron microscopy
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TLDR
Two computer programs are presented, CTFFIND3 and CTFTILT, which determine defocus parameters from images of untilted specimens, as well as defocus and tilt parameters from image of tilted specimens, respectively, using a simple algorithm.About:
This article is published in Journal of Structural Biology.The article was published on 2003-06-01. It has received 1480 citations till now. The article focuses on the topics: Tilt (optics) & Contrast transfer function.read more
Citations
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Journal ArticleDOI
Proteomic characterization of archaeal ribosomes reveals the presence of novel archaeal-specific ribosomal proteins
Viter Márquez,Thomas Fröhlich,Jean Paul Armache,Jean Paul Armache,Daniel Sohmen,Daniel Sohmen,Alexandra Dönhöfer,Alexandra Dönhöfer,Aleksandra Mikolajka,Aleksandra Mikolajka,Otto Berninghausen,Otto Berninghausen,Michael Thomm,Roland Beckmann,Roland Beckmann,Georg J. Arnold,Daniel N. Wilson,Daniel N. Wilson +17 more
TL;DR: A comprehensive two-dimensional PAGE and mass spectrometry analysis of archaeal ribosomes isolated from the thermophilic Pyrobaculum aerophilum and the thermoacidophilic Sulfolobus acidocaldarius Crenarchaeota was performed in this paper.
Journal ArticleDOI
Characterization of a potent and highly unusual minimally enhancing antibody directed against dengue virus.
Max Renner,Aleksandra Flanagan,Wanwisa Dejnirattisai,Chunya Puttikhunt,Watchara Kasinrerk,Piyada Supasa,Wiyada Wongwiwat,Kriangkrai Chawansuntati,Thaneeya Duangchinda,Alison E. Cowper,Claire M. Midgley,Prida Malasit,Juha T. Huiskonen,Juthathip Mongkolsapaya,Juthathip Mongkolsapaya,Gavin R. Screaton,Jonathan M. Grimes +16 more
TL;DR: It is shown that 3H5 shows resilient binding in endosomal pH conditions and neutralizes at low occupancy, which is proposed to be the primary mechanism of how ADE is avoided.
Book ChapterDOI
Semiautomatic, high-throughput, high-resolution protocol for three-dimensional reconstruction of single particles in electron microscopy.
Carlos Oscar S. Sorzano,J. M. de la Rosa Trevín,Joaquín Otón,J. J. Vega,J. Cuenca,A. Zaldívar-Peraza,Josué Gómez-Blanco,Javier Vargas,A. Quintana,Roberto Marabini,José María Carazo +10 more
TL;DR: This chapter describes the steps needed for reconstructing the three-dimensional structure of a macromolecular complex starting from its projections collected in electron micrographs and shows that in the case of high-quality, homogeneous datasets with a priori knowledge about the initial volume, a high-resolution 3D reconstruction in less than 1 day can be had.
Journal ArticleDOI
A PH Domain in ACAP1 Possesses Key Features of the BAR Domain in Promoting Membrane Curvature
Xiaoyun Pang,Jun Fan,Yan Zhang,Kai Zhang,Bingquan Gao,Jun Ma,Jian Li,Yuchen Deng,Qiangjun Zhou,Edward H. Egelman,Victor W. Hsu,Victor W. Hsu,Fei Sun +12 more
TL;DR: It is shown that the BAR domain adjacent to the PH domain can neither bind membrane nor impart curvature, but instead requires a neighboring PH domain to achieve these functions.
Journal ArticleDOI
Self-organization of the vesicular stomatitis virus nucleocapsid into a bullet shape
Ambroise Desfosses,Ambroise Desfosses,Euripedes A. Ribeiro,Guy Schoehn,Guy Schoehn,Danielle Blondel,Delphine Guilligay,Marc Jamin,Rob W.H. Ruigrok,Irina Gutsche +9 more
TL;DR: This work scrutinizes the morphology of purified and recombinant nucleocapsids of vesicular stomatitis virus in vitro, elucidate pH and ionic strength conditions for their folding into conical tips and further growth into whole bullets, and provides cryo-electron microscopy reconstructions of the bullet tip and the helical trunk.
References
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Journal ArticleDOI
Three-dimensional model of purple membrane obtained by electron microscopy
Richard Henderson,P. N. T. Unwin +1 more
TL;DR: A 7-Å resolution map of the purple membrane has been obtained by electron microscopy of tilted, unstained specimens and shows that Lipid bilayer regions fill the spaces between the protein molecules.
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Structure of the alpha beta tubulin dimer by electron crystallography.
TL;DR: An atomic model of the αβ tubulin dimer fitted to a 3.7-Å density map obtained by electron crystallography of zinc-induced tubulin sheets is presented.
Journal ArticleDOI
Structural determinants of water permeation through aquaporin-1.
Kazuyoshi Murata,Kaoru Mitsuoka,Teruhisa Hirai,Thomas Walz,Peter Agre,J. B. Heymann,Andreas Engel,Yoshinori Fujiyoshi +7 more
TL;DR: An atomic model of human red cell AQP1 is described, providing a possible molecular explanation to a longstanding puzzle in physiology—how membranes can be freely permeable to water but impermeable to protons.
Journal ArticleDOI
Structure of dengue virus: implications for flavivirus organization, maturation, and fusion.
Richard J. Kuhn,Wei Zhang,Michael G. Rossmann,Sergei V. Pletnev,Jeroen Corver,Edith M. Lenches,Christopher T. Jones,Suchetana Mukhopadhyay,Paul R. Chipman,Ellen G. Strauss,Timothy S. Baker,James H. Strauss +11 more
TL;DR: The first structure of a flavivirus has been determined by using a combination of cryoelectron microscopy and fitting of the known structure of glycoprotein E into the electron density map, suggesting that flaviviruses employ a fusion mechanism in which the distal beta barrels of domain II of the glycop Protein E are inserted into the cellular membrane.
Journal ArticleDOI
A ratchet-like inter-subunit reorganization of the ribosome during translocation.
TL;DR: Three-dimensional cryo-electron microscopy maps of the Escherichia coli 70S ribosome in various functional states show that both EF-G binding and subsequent GTP hydrolysis lead to ratchet-like rotations of the small 30S sub unit relative to the large 50S subunit, indicating a two-step mechanism of translocation.