Journal ArticleDOI
Accurate determination of local defocus and specimen tilt in electron microscopy
Reads0
Chats0
TLDR
Two computer programs are presented, CTFFIND3 and CTFTILT, which determine defocus parameters from images of untilted specimens, as well as defocus and tilt parameters from image of tilted specimens, respectively, using a simple algorithm.About:
This article is published in Journal of Structural Biology.The article was published on 2003-06-01. It has received 1480 citations till now. The article focuses on the topics: Tilt (optics) & Contrast transfer function.read more
Citations
More filters
Journal ArticleDOI
Transport mechanism of P4 ATPase phosphatidylcholine flippases.
Lin Bai,Qinglong You,Bhawik Kumar Jain,H. Diessel Duan,Amanda Kovach,Todd R. Graham,Huilin Li +6 more
TL;DR: The P4 ATPases may have retained an overall transport mechanism while evolving distinct features for different lipid substrates while retaining a conserved mechanism among P 4 ATPases.
Journal ArticleDOI
Structural Basis for Linezolid Binding Site Rearrangement in the Staphylococcus aureus Ribosome.
Matthew J. Belousoff,Z. Eyal,Mazdak Radjainia,Tofayel Ahmed,Rebecca S. Bamert,Donna Matzov,Anat Bashan,Ella Zimmerman,Satabdi Mishra,David R. Cameron,Hans Elmlund,Hans Elmlund,Anton Y. Peleg,Anton Y. Peleg,Shashi Bhushan,Trevor Lithgow,Ada Yonath +16 more
TL;DR: High-resolution structural information demonstrated that a single amino acid deletion in ribosomal protein uL3 confers linezolid resistance despite being located 24 Å away from the lineZolid binding pocket in the peptidyl-transferase center.
Journal ArticleDOI
A Janus-Faced IM30 Ring Involved in Thylakoid Membrane Fusion Is Assembled from IM30 Tetramers
Michael Saur,Raoul Hennig,Phoebe Young,Kristiane Rusitzka,Nadja Hellmann,Jennifer Heidrich,Nina Morgner,Jürgen Markl,Dirk Schneider +8 more
TL;DR: It is proposed that a two-sided IM30 ring complex connects two opposing membranes, finally resulting in membrane fusion, and that IM30-mediated membrane fusion requires a Janus-faced IM30 rings.
Journal ArticleDOI
The Genomes, Proteomes, and Structures of Three Novel Phages That Infect the Bacillus cereus Group and Carry Putative Virulence Factors
Julianne H. Grose,David M. Belnap,Jordan D. Jensen,Andrew D. Mathis,John T. Prince,Bryan D. Merrill,Sandra H. Burnett,Donald P. Breakwell +7 more
TL;DR: The detailed characterization of host range and superinfection, together with results of genomic, proteomic, and structural analyses, reveal several putative virulence factors as well as the ability of these phages to infect different pathogenic species.
Journal ArticleDOI
Three-dimensional structure of victorivirus HvV190S suggests coat proteins in most totiviruses share a conserved core.
TL;DR: Transmission electron cryomicroscopy and three-dimensional image reconstruction are used to determine the structures of HvV190S virions and two types of virus-like particles and predict most if not all totiviruses including, Leishmania RNA virus 1, to be similar as well.
References
More filters
Journal ArticleDOI
Three-dimensional model of purple membrane obtained by electron microscopy
Richard Henderson,P. N. T. Unwin +1 more
TL;DR: A 7-Å resolution map of the purple membrane has been obtained by electron microscopy of tilted, unstained specimens and shows that Lipid bilayer regions fill the spaces between the protein molecules.
Journal ArticleDOI
Structure of the alpha beta tubulin dimer by electron crystallography.
TL;DR: An atomic model of the αβ tubulin dimer fitted to a 3.7-Å density map obtained by electron crystallography of zinc-induced tubulin sheets is presented.
Journal ArticleDOI
Structural determinants of water permeation through aquaporin-1.
Kazuyoshi Murata,Kaoru Mitsuoka,Teruhisa Hirai,Thomas Walz,Peter Agre,J. B. Heymann,Andreas Engel,Yoshinori Fujiyoshi +7 more
TL;DR: An atomic model of human red cell AQP1 is described, providing a possible molecular explanation to a longstanding puzzle in physiology—how membranes can be freely permeable to water but impermeable to protons.
Journal ArticleDOI
Structure of dengue virus: implications for flavivirus organization, maturation, and fusion.
Richard J. Kuhn,Wei Zhang,Michael G. Rossmann,Sergei V. Pletnev,Jeroen Corver,Edith M. Lenches,Christopher T. Jones,Suchetana Mukhopadhyay,Paul R. Chipman,Ellen G. Strauss,Timothy S. Baker,James H. Strauss +11 more
TL;DR: The first structure of a flavivirus has been determined by using a combination of cryoelectron microscopy and fitting of the known structure of glycoprotein E into the electron density map, suggesting that flaviviruses employ a fusion mechanism in which the distal beta barrels of domain II of the glycop Protein E are inserted into the cellular membrane.
Journal ArticleDOI
A ratchet-like inter-subunit reorganization of the ribosome during translocation.
TL;DR: Three-dimensional cryo-electron microscopy maps of the Escherichia coli 70S ribosome in various functional states show that both EF-G binding and subsequent GTP hydrolysis lead to ratchet-like rotations of the small 30S sub unit relative to the large 50S subunit, indicating a two-step mechanism of translocation.