Journal ArticleDOI
Accurate determination of local defocus and specimen tilt in electron microscopy
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TLDR
Two computer programs are presented, CTFFIND3 and CTFTILT, which determine defocus parameters from images of untilted specimens, as well as defocus and tilt parameters from image of tilted specimens, respectively, using a simple algorithm.About:
This article is published in Journal of Structural Biology.The article was published on 2003-06-01. It has received 1480 citations till now. The article focuses on the topics: Tilt (optics) & Contrast transfer function.read more
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Structural Insights Into Viral Determinants of Nematode Mediated Grapevine Fanleaf Virus Transmission.
Pascale Schellenberger,Claude Sauter,Bernard Lorber,Patrick Bron,Stefano Trapani,Stefano Trapani,Marc Bergdoll,Aurélie Marmonier,Corinne Schmitt-Keichinger,Olivier Lemaire,Gérard Demangeat,Christophe Ritzenthaler +11 more
TL;DR: It is proposed that perturbation of the electrostatic landscape of this pocket affects the interaction of the virion with specific receptors of the nematode's feeding apparatus, and thereby severely diminishes its transmission efficiency.
Journal ArticleDOI
Structural Snapshots of 26S Proteasome Reveal Tetraubiquitin-Induced Conformations.
Zhanyu Ding,Cong Xu,Indrajit Sahu,Yifan Wang,Zhenglin Fu,Min Huang,Catherine C. L. Wong,Catherine C. L. Wong,Michael H. Glickman,Yao Cong +9 more
TL;DR: The authors' structures indicate that tetraUb induced conformational changes in proteasome could initiate substrate degradation, and propose a CP gate-opening mechanism involving the propagation of the motion of the lid to the gate through the Rpn6-α2 interaction.
Journal ArticleDOI
Structure of a human pre-40S particle points to a role for RACK1 in the final steps of 18S rRNA processing
Natacha Larburu,Christian Montellese,Marie-Françoise O'Donohue,Ulrike Kutay,Pierre-Emmanuel Gleizes,Célia Plisson-Chastang +5 more
TL;DR: This first structural analysis of a human pre-ribosomal particle sets the grounds for high-resolution studies of conformational transitions accompanying ribosomal subunit maturation.
Journal ArticleDOI
Quaternary structure of the specific p53–DNA complex reveals the mechanism of p53 mutant dominance
Ricardo Aramayo,Michael B. Sherman,Kathryne Brownless,Rudi Lurz,Andrei L. Okorokov,Elena V. Orlova +5 more
TL;DR: The cryo electron microscopy structure of the full-length p53 tetramer bound to a DNA-encoding transcription factor response element (RE) at a resolution of 21 Å indicates that the Achilles’ heel of p53 is in its dimer-of-dimers organization, thus the tetramer activity can be negated by mutation in only one allele followed by tumourigenesis.
Journal ArticleDOI
Structural basis for assembly and function of a diatom photosystem I-light-harvesting supercomplex.
Ryo Nagao,Koji Kato,Kentaro Ifuku,Takehiro Suzuki,Minoru Kumazawa,Ikuo Uchiyama,Yasuhiro Kashino,Naoshi Dohmae,Seiji Akimoto,Jian Ren Shen,Naoyuki Miyazaki,Naoyuki Miyazaki,Fusamichi Akita,Fusamichi Akita +13 more
TL;DR: The authors present the cryo-EM structure of the photosystem I-FCP (PSI-FCPI) supercomplex isolated from the marine centric diatom Chaetoceros gracilis that contains 16 FCPI subunits surrounding the PSI core and discuss possible excitation energy transfer pathways.
References
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Journal ArticleDOI
Three-dimensional model of purple membrane obtained by electron microscopy
Richard Henderson,P. N. T. Unwin +1 more
TL;DR: A 7-Å resolution map of the purple membrane has been obtained by electron microscopy of tilted, unstained specimens and shows that Lipid bilayer regions fill the spaces between the protein molecules.
Journal ArticleDOI
Structure of the alpha beta tubulin dimer by electron crystallography.
TL;DR: An atomic model of the αβ tubulin dimer fitted to a 3.7-Å density map obtained by electron crystallography of zinc-induced tubulin sheets is presented.
Journal ArticleDOI
Structural determinants of water permeation through aquaporin-1.
Kazuyoshi Murata,Kaoru Mitsuoka,Teruhisa Hirai,Thomas Walz,Peter Agre,J. B. Heymann,Andreas Engel,Yoshinori Fujiyoshi +7 more
TL;DR: An atomic model of human red cell AQP1 is described, providing a possible molecular explanation to a longstanding puzzle in physiology—how membranes can be freely permeable to water but impermeable to protons.
Journal ArticleDOI
Structure of dengue virus: implications for flavivirus organization, maturation, and fusion.
Richard J. Kuhn,Wei Zhang,Michael G. Rossmann,Sergei V. Pletnev,Jeroen Corver,Edith M. Lenches,Christopher T. Jones,Suchetana Mukhopadhyay,Paul R. Chipman,Ellen G. Strauss,Timothy S. Baker,James H. Strauss +11 more
TL;DR: The first structure of a flavivirus has been determined by using a combination of cryoelectron microscopy and fitting of the known structure of glycoprotein E into the electron density map, suggesting that flaviviruses employ a fusion mechanism in which the distal beta barrels of domain II of the glycop Protein E are inserted into the cellular membrane.
Journal ArticleDOI
A ratchet-like inter-subunit reorganization of the ribosome during translocation.
TL;DR: Three-dimensional cryo-electron microscopy maps of the Escherichia coli 70S ribosome in various functional states show that both EF-G binding and subsequent GTP hydrolysis lead to ratchet-like rotations of the small 30S sub unit relative to the large 50S subunit, indicating a two-step mechanism of translocation.