Journal ArticleDOI
Accurate determination of local defocus and specimen tilt in electron microscopy
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TLDR
Two computer programs are presented, CTFFIND3 and CTFTILT, which determine defocus parameters from images of untilted specimens, as well as defocus and tilt parameters from image of tilted specimens, respectively, using a simple algorithm.About:
This article is published in Journal of Structural Biology.The article was published on 2003-06-01. It has received 1480 citations till now. The article focuses on the topics: Tilt (optics) & Contrast transfer function.read more
Citations
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Virion Structure of Baboon Reovirus, a Fusogenic Orthoreovirus That Lacks an Adhesion Fiber
Xiaodong Yan,Kristin N. Parent,Russell P. Goodman,Russell P. Goodman,Jinghua Tang,Jingyun Shou,Max L. Nibert,Roy Duncan,Timothy S. Baker +8 more
TL;DR: It is concluded that the evolution of ortho- and aquareoviruses has included a series of discrete gains or losses of particular components, several of which cross taxonomic boundaries.
Journal ArticleDOI
HIV-1 VRC01 Germline-Targeting Immunogens Select Distinct Epitope-Specific B Cell Receptors.
Yu-Ru Lin,K. Rachael Parks,K. Rachael Parks,Connor Weidle,Anika S. Naidu,Arineh Khechaduri,Andrew O. Riker,Brittany Takushi,Jung-Ho Chun,Andrew J. Borst,David Veesler,Andrew B. Stuart,Parul Agrawal,Matthew D. Gray,Marie Pancera,Marie Pancera,Po-Ssu Huang,Leonidas Stamatatos,Leonidas Stamatatos +18 more
TL;DR: The study indicates that the design of effective immunogens to activate B cell receptors leading to protective HIV-1 antibodies will require a better understanding of how the biophysical properties of the epitope and its surrounding surface on the germline-targeting immunogen influence its interaction with the available receptor variants in vivo.
Journal ArticleDOI
3D electron microscopy of biological nanomachines: principles and applications
Carlos Oscar S. Sorzano,Slavica Jonic,Magali Cottevieille,Eric Larquet,Nicolas Boisset,S. Marco +5 more
TL;DR: The principles and main application domains of 3D transmission electron microscopy in structural biology are presented, and current developments needed in this field are surveyed.
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Cryo-EM structure of TFIIH/Rad4-Rad23-Rad33 in damaged DNA opening in nucleotide excision repair.
Trevor van Eeuwen,Yoonjung Shim,Hee Jong Kim,Tingting Zhao,Shrabani Basu,Benjamin A. Garcia,Craig D. Kaplan,Jung Hyun Min,Kenji Murakami +8 more
TL;DR: In this article, the structure of an NER initiation complex containing Rad4-Rad23-Rad33 (yeast homologue of XPC-RAD23B-CETN2) and 7-subunit core TFIIH assembled on a carcinogen-DNA adduct lesion at 3.9-9.2
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Crystal Structures of Two Immune Complexes Identify Determinants for Viral Infectivity and Type-Specific Neutralization of Human Papillomavirus.
Zhihai Li,Daning Wang,Ying Gu,Shuo Song,Maozhou He,Shi Jingjie,Xinlin Liu,Shuangping Wei,Jinjin Li,Hai Yu,Qingbing Zheng,Xiaodong Yan,Xiaodong Yan,Timothy S. Baker,Jun Zhang,Jason S. McLellan,Shaowei Li,Ningshao Xia +17 more
TL;DR: Two antibodies are described, A12A3 and 28F10, that specifically bind to and neutralize HPV58 and HPV59, respectively, through two distinct binding stoichiometries, providing molecular insights into potential structural determinants of HPV required for infectivity and type specificity.
References
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Journal ArticleDOI
Three-dimensional model of purple membrane obtained by electron microscopy
Richard Henderson,P. N. T. Unwin +1 more
TL;DR: A 7-Å resolution map of the purple membrane has been obtained by electron microscopy of tilted, unstained specimens and shows that Lipid bilayer regions fill the spaces between the protein molecules.
Journal ArticleDOI
Structure of the alpha beta tubulin dimer by electron crystallography.
TL;DR: An atomic model of the αβ tubulin dimer fitted to a 3.7-Å density map obtained by electron crystallography of zinc-induced tubulin sheets is presented.
Journal ArticleDOI
Structural determinants of water permeation through aquaporin-1.
Kazuyoshi Murata,Kaoru Mitsuoka,Teruhisa Hirai,Thomas Walz,Peter Agre,J. B. Heymann,Andreas Engel,Yoshinori Fujiyoshi +7 more
TL;DR: An atomic model of human red cell AQP1 is described, providing a possible molecular explanation to a longstanding puzzle in physiology—how membranes can be freely permeable to water but impermeable to protons.
Journal ArticleDOI
Structure of dengue virus: implications for flavivirus organization, maturation, and fusion.
Richard J. Kuhn,Wei Zhang,Michael G. Rossmann,Sergei V. Pletnev,Jeroen Corver,Edith M. Lenches,Christopher T. Jones,Suchetana Mukhopadhyay,Paul R. Chipman,Ellen G. Strauss,Timothy S. Baker,James H. Strauss +11 more
TL;DR: The first structure of a flavivirus has been determined by using a combination of cryoelectron microscopy and fitting of the known structure of glycoprotein E into the electron density map, suggesting that flaviviruses employ a fusion mechanism in which the distal beta barrels of domain II of the glycop Protein E are inserted into the cellular membrane.
Journal ArticleDOI
A ratchet-like inter-subunit reorganization of the ribosome during translocation.
TL;DR: Three-dimensional cryo-electron microscopy maps of the Escherichia coli 70S ribosome in various functional states show that both EF-G binding and subsequent GTP hydrolysis lead to ratchet-like rotations of the small 30S sub unit relative to the large 50S subunit, indicating a two-step mechanism of translocation.