Journal ArticleDOI
Accurate determination of local defocus and specimen tilt in electron microscopy
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TLDR
Two computer programs are presented, CTFFIND3 and CTFTILT, which determine defocus parameters from images of untilted specimens, as well as defocus and tilt parameters from image of tilted specimens, respectively, using a simple algorithm.About:
This article is published in Journal of Structural Biology.The article was published on 2003-06-01. It has received 1480 citations till now. The article focuses on the topics: Tilt (optics) & Contrast transfer function.read more
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Acquisition of functions on the outer capsid surface during evolution of double-stranded RNA fungal viruses.
Carlos P. Mata,Daniel Luque,Daniel Luque,Josué Gómez-Blanco,Javier M. Rodríguez,José M. González,Nobuhiro Suzuki,Said A. Ghabrial,José L. Carrascosa,Benes L. Trus,José R. Castón +10 more
TL;DR: The results indicate that the intimate virus-fungus partnership has altered the capsid genome-protective and/or receptor-binding functions in Rosellinia necatrix quadrivirus 1, indicating that Fungal virus evolution has tended to allocate enzyme activities to the virus capsid outer surface.
Journal ArticleDOI
The Architecture of Yeast DNA Polymerase ζ
Yacob Gomez-Llorente,Radhika Malik,Rinku Jain,Jayati Roy Choudhury,Robert E. Johnson,Louise Prakash,Satya Prakash,Iban Ubarretxena-Belandia,Aneel K. Aggarwal +8 more
TL;DR: It is shown that Polζ-d has a bilobal architecture resembling the replicative polymerases and that Pol32 lies in proximity to Rev7, and a structural framework for understanding their roles in DNA damage bypass is provided.
Journal ArticleDOI
Structure of Yin Yang 1 Oligomers That Cooperate with RuvBL1-RuvBL2 ATPases
Andrés López-Perrote,Hanan E. Alatwi,Eva Torreira,A. A. Ismail,Silvia Ayora,Jessica A. Downs,Oscar Llorca +6 more
TL;DR: It is shown that YY1 and the ATPase activity of RuvBL2 are required for RAD51 foci formation during homologous recombination, and these findings suggest that Yy1-RuvBL1- RuvBL2 complexes could contribute to functions beyond transcription.
Journal ArticleDOI
Two p53 tetramers bind one consensus DNA response element
TL;DR: The EM structural analysis revealed that two p53 tetramers bind one DNA RE simultaneously with DNA positioned between them, demonstrating a mode different from that assumed previously for the p53-DNA interaction and suggesting important biological implications on p53 activity as a transcriptional regulator of cellular response to stress.
Journal ArticleDOI
Structures of Mycobacterium smegmatis 70S ribosomes in complex with HPF, tmRNA, and P-tRNA
TL;DR: The authors' structural data shows a Mycobacteria-specific H54a-bS1 interaction which seems to prevent subunit dissociation and degradation during hibernation without the formation of 100S dimer, which indicates a new role of bS1 protein in 70S protection during hibernating in Myc Cobacteria in addition to its conserved function during translation initiation.
References
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Journal ArticleDOI
Three-dimensional model of purple membrane obtained by electron microscopy
Richard Henderson,P. N. T. Unwin +1 more
TL;DR: A 7-Å resolution map of the purple membrane has been obtained by electron microscopy of tilted, unstained specimens and shows that Lipid bilayer regions fill the spaces between the protein molecules.
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Structure of the alpha beta tubulin dimer by electron crystallography.
TL;DR: An atomic model of the αβ tubulin dimer fitted to a 3.7-Å density map obtained by electron crystallography of zinc-induced tubulin sheets is presented.
Journal ArticleDOI
Structural determinants of water permeation through aquaporin-1.
Kazuyoshi Murata,Kaoru Mitsuoka,Teruhisa Hirai,Thomas Walz,Peter Agre,J. B. Heymann,Andreas Engel,Yoshinori Fujiyoshi +7 more
TL;DR: An atomic model of human red cell AQP1 is described, providing a possible molecular explanation to a longstanding puzzle in physiology—how membranes can be freely permeable to water but impermeable to protons.
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Structure of dengue virus: implications for flavivirus organization, maturation, and fusion.
Richard J. Kuhn,Wei Zhang,Michael G. Rossmann,Sergei V. Pletnev,Jeroen Corver,Edith M. Lenches,Christopher T. Jones,Suchetana Mukhopadhyay,Paul R. Chipman,Ellen G. Strauss,Timothy S. Baker,James H. Strauss +11 more
TL;DR: The first structure of a flavivirus has been determined by using a combination of cryoelectron microscopy and fitting of the known structure of glycoprotein E into the electron density map, suggesting that flaviviruses employ a fusion mechanism in which the distal beta barrels of domain II of the glycop Protein E are inserted into the cellular membrane.
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A ratchet-like inter-subunit reorganization of the ribosome during translocation.
TL;DR: Three-dimensional cryo-electron microscopy maps of the Escherichia coli 70S ribosome in various functional states show that both EF-G binding and subsequent GTP hydrolysis lead to ratchet-like rotations of the small 30S sub unit relative to the large 50S subunit, indicating a two-step mechanism of translocation.