Journal ArticleDOI
Avoiding false positives with PCR
S Kwok,Russell Higuchi +1 more
Reads0
Chats0
TLDR
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment and adherence to a strict set of protocols can avoid disaster.Abstract:
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment. Tidiness and adherence to a strict set of protocols can avoid disaster.read more
Citations
More filters
Journal ArticleDOI
Infection with a plasmid-free variant Chlamydia related to Chlamydia trachomatis identified by using multiple assays for nucleic acid detection.
Q An,G Radcliffe,R Vassallo,D Buxton,W. J. O'brien,Dale A. Pelletier,William G. Weisburg,J D Klinger,D. M. Olive +8 more
TL;DR: Clinical samples in transport media from 40 patients exhibiting pathologies potentially caused by Chlamydia trachomatis infection were analyzed for chlamydial nucleic acid, and the results were compared with those of culture.
Journal ArticleDOI
Identification of Bordetella pertussis infection by shared-primer PCR.
Z M Li,D. L. Jansen,T. M. Finn,S. A. Halperin,A. Kasina,S. P. O'connor,T. Aoyama,Charles R. Manclark,M J Brennan +8 more
TL;DR: The specific and sensitive PCR method described here should be useful for both the clinical diagnosis of pertussis and case identification in vaccine trials.
Book ChapterDOI
Real-time quantitative PCR, pathogen detection and MIQE
TL;DR: The "Minimal Information for the Publication of real-time PCR" (MIQE) guidelines aim to improve both the reliability of assay design as well as the transparency of reporting, essential conditions if qPCR is to remain the benchmark technology for molecular diagnosis.
Journal ArticleDOI
Hepatitis B Virus DNA in Persons with Isolated Antibody to Hepatitis B Core Antigen Who Subsequently Received Hepatitis B Vaccine
A. Eduardo Silva,Brian J. McMahon,Alan J. Parkinson,Maria H. Sjogren,Jay H. Hoofnagle,Adrian M. Di Bisceglie +5 more
TL;DR: Serum samples from 133 persons who were positive only for antibody to hepatitis B core antigen (anti-HBc) by enzyme immunoassay were retested for seromarkers of hepatitis B virus (HBV) by radioimmunoASSay and for HBV DNA by polymerase chain reaction analysis.
Journal ArticleDOI
Differential susceptibilities of Anopheles albimanus and Anopheles pseudopunctipennis to infections with coindigenous Plasmodium vivax variants VK210 and VK247 in southern Mexico.
Lilia González-Cerón,Mario H. Rodriguez,Jose C. Nettel,Cuauhtemoc Villarreal,Kevin C. Kain,Juan E. Hernandez +5 more
TL;DR: The susceptibilities to coindigenous Plasmodium vivaxof colonized Anopheles albimanus and Anophele pseudopunctipennis from southern Mexico were investigated by simultaneous feeding with infected blood obtained from patients.
References
More filters
Journal ArticleDOI
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
Randall Keichi Saiki,David H. Gelfand,Susanne Stoffel,Stephen J. Scharf,Russell Higuchi,Glenn Thomas Horn,Kary B. Mullis,Henry A. Erlich +7 more
TL;DR: A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified.
Book ChapterDOI
Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.
Kary B. Mullis,Fred A. Faloona +1 more
TL;DR: A method whereby a nucleic acid sequence can be exponentially amplified in vitro is described in the chapter, and the possibility of utilizing a heat-stable DNA polymerase is explored so as to avoid the need for addition of new enzyme after each cycle of thermal denaturation.
Journal ArticleDOI
DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells
Chin-Yih Ou,Shirley Kwok,Sheila W. Mitchell,David Henry Mack,John J. Sninsky,John W. Krebs,Paul M. Feorino,Donna T. Warfield,Gerald Schochetman +8 more
TL;DR: This method of DNA amplification made it possible to obtain results within 3 days, whereas virus isolation takes up to 3 to 4 weeks, and may therefore be used to complement or replace virus isolation as a routine means of determining HIV-1 infection.
Journal ArticleDOI
DNA typing from single hairs
TL;DR: Three different means of DNA typing are used for the determination of amplified DNA fragment length differences, hybridization with allele-specific oligonucleotide probes, and direct DNA sequencing on single human hairs to detect genetically variable mitochondrial and nuclear DNA sequences.
Journal ArticleDOI
Amplification and analysis of DNA sequences in single human sperm and diploid cells
Honghua Li,Ulf B. Gyllensten,Xiangfeng Cui,Randall Keichi Saiki,Henry A. Erlich,Norman Arnheim +5 more
TL;DR: The use of the polymerase chain reaction for analysing DNA sequences in individual diploid cells and human sperm shows that two genetic loci can be co-amplified from a single sperm, which may allow the analysis of previously inaccessible genetic phenomena.