Journal ArticleDOI
Avoiding false positives with PCR
S Kwok,Russell Higuchi +1 more
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TLDR
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment and adherence to a strict set of protocols can avoid disaster.Abstract:
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment. Tidiness and adherence to a strict set of protocols can avoid disaster.read more
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Journal ArticleDOI
Are haemochromatosis mutations related to the severity of liver disease in hepatitis C virus infection
Ana de Lourdes Candolo Martinelli,Rendrik F. Franco,Márcia Guimarães Villanova,José Fernando de Castro Figueiredo,Marie Secaf,Marli Haydee Tavella,Leandra Naira Zambelli Ramalho,Sérgio Zucoloto,Marco Antonio Zago +8 more
TL;DR: Patients with chronic HCV infection carrying HFE mutations tend to present more evident body iron accumulation and a higher degree of necroinflammatory activity and fibrosis in the liver, and HFE gene mutations might be an additional factor to be considered among those implicated in the determination of a worse prognosis of the liver disease in chronicHCV infection.
Journal ArticleDOI
Evaluation of the ICT Malaria P.f/P.v and the OptiMal Rapid Diagnostic Tests for Malaria in Febrile Returned Travellers
E. G. Playford,J. Walker +1 more
TL;DR: Despite the high sensitivity of the ICT P.f/P.v assay for P. falciparum malaria, caution is warranted before RDTs are widely adopted for the diagnosis of malaria in nonimmune patients or in countries where malaria is not endemic.
Journal Article
A possible contributory role of BK virus infection in neuroblastoma development.
Trond Flægstad,Per Arne Andresen,John Inge Johnsen,Samuel Kofi Asomani,Gunn-Eli Jørgensen,Somasuntharam Vignarajan,Anita Kjuul,Per Kogner,Terje Traavik +8 more
TL;DR: The presence and expression of BK virus in neuroblastomas, but not in normal adrenal medulla, and colocalization and binding to p53, suggest that this virus may play a contributory role in the development of this neoplasm.
Journal ArticleDOI
The der(11)-encoded MLL/AF-4 fusion transcript is consistently detected in t(4;11)(q21;q23)-containing acute lymphoblastic leukemia
James R. Downing,David R. Head,Susana C. Raimondi,Andrew J. Carroll,A. M. Curcio-Brint,T. A. Motroni,M. G. Hulshof,DJ Pullen,P H Domer +8 more
TL;DR: The data suggest that the critical chimeric gene product involved in the establishment of the leukemic clone is derived from the der(11) chromosome, and demonstrate the utility of the RT-PCR assay for the der (11)encoded message both for diagnosing t(4;11)-containing leukemia and for monitoring patients for minimal residual disease.
Journal ArticleDOI
Polymerase chain reaction-based diagnosis of Mediterranean spotted fever in serum and tissue samples.
TL;DR: The results demonstrate that sera and tissue samples are suitable specimens for the nested PCR tests, especially in fatal cases.
References
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Journal ArticleDOI
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
Randall Keichi Saiki,David H. Gelfand,Susanne Stoffel,Stephen J. Scharf,Russell Higuchi,Glenn Thomas Horn,Kary B. Mullis,Henry A. Erlich +7 more
TL;DR: A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified.
Book ChapterDOI
Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.
Kary B. Mullis,Fred A. Faloona +1 more
TL;DR: A method whereby a nucleic acid sequence can be exponentially amplified in vitro is described in the chapter, and the possibility of utilizing a heat-stable DNA polymerase is explored so as to avoid the need for addition of new enzyme after each cycle of thermal denaturation.
Journal ArticleDOI
DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells
Chin-Yih Ou,Shirley Kwok,Sheila W. Mitchell,David Henry Mack,John J. Sninsky,John W. Krebs,Paul M. Feorino,Donna T. Warfield,Gerald Schochetman +8 more
TL;DR: This method of DNA amplification made it possible to obtain results within 3 days, whereas virus isolation takes up to 3 to 4 weeks, and may therefore be used to complement or replace virus isolation as a routine means of determining HIV-1 infection.
Journal ArticleDOI
DNA typing from single hairs
TL;DR: Three different means of DNA typing are used for the determination of amplified DNA fragment length differences, hybridization with allele-specific oligonucleotide probes, and direct DNA sequencing on single human hairs to detect genetically variable mitochondrial and nuclear DNA sequences.
Journal ArticleDOI
Amplification and analysis of DNA sequences in single human sperm and diploid cells
Honghua Li,Ulf B. Gyllensten,Xiangfeng Cui,Randall Keichi Saiki,Henry A. Erlich,Norman Arnheim +5 more
TL;DR: The use of the polymerase chain reaction for analysing DNA sequences in individual diploid cells and human sperm shows that two genetic loci can be co-amplified from a single sperm, which may allow the analysis of previously inaccessible genetic phenomena.