Journal ArticleDOI
Avoiding false positives with PCR
S Kwok,Russell Higuchi +1 more
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TLDR
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment and adherence to a strict set of protocols can avoid disaster.Abstract:
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment. Tidiness and adherence to a strict set of protocols can avoid disaster.read more
Citations
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Review Paper. Ancient DNA
Eske Willerslev,Alan Cooper +1 more
TL;DR: Initial results have revealed surprisingly complex population histories, and indicate that modern phylogeographic studies may give misleading impressions about even the recent evolutionary past.
Journal ArticleDOI
The causal link between human papillomavirus and invasive cervical cancer: A population-based case-control study in colombia and spain
Nubia Muñoz,F. X. Bosch,S de Sanjosé,Luis Alberto Tafur,Isabel Izarzugaza,Miguel Gili,P. Viladiu,Carmen Navarro,C. Martos,Nieves Ascunce,L. C. Gonzalez,John M. Kaldor,E. Guerrero,Attila T. Lorincz,M. Santamaria,P. Alonso De Ruiz,Nubia Aristizabal,Keerti V. Shah +17 more
TL;DR: There is a very strong association between HPV 16, 18, 31, 33 and 35 and invasive cervical cancer and that this association is probably causal.
Journal ArticleDOI
Chromosome translocations and covert leukemic clones are generated during normal fetal development
Hiroshi Mori,Susan M. Colman,Zhijian Xiao,Anthony M. Ford,Lyn E. Healy,Craig Donaldson,Jill Hows,Cristina Navarrete,Mel Greaves +8 more
TL;DR: Using parallel reverse transcriptase–PCR and real-time PCR screening, it is found that the common leukemia fusion genes, TEL-AML1 or AML1-ETO, are present in cord bloods at a frequency that is 100-fold greater than the risk of the corresponding leukemia.
Journal ArticleDOI
Rapid diagnosis of herpes simplex encephalitis by nested polymerase chain reaction assay of cerebrospinal fluid.
TL;DR: A polymerase chain reaction (PCR) assay with two "nested" primer pairs was developed for the amplification of herpes simplex virus DNA in cerebrospinal fluid (CSF) and southern blotting was used to confirm the specificity of the amplification.
Journal ArticleDOI
Detection of hepatitis C viral sequences in blood donations by "nested" polymerase chain reaction and prediction of infectivity.
Jeremy A. Garson,Richard S. Tedder,Moya Briggs,Philip W. Tuke,J. A. Glazebrook,A. Trute,D. Parker,J.A.J. Barbara,M. Contreras,S. Aloysius +9 more
TL;DR: Stored serum samples from blood donors who had been involved in three episodes of post-transfusion NANBH in 1981 also contained hepatitis C viral sequences, which seem to be a better predictor of infectivity than the presence of anti-C100 alone.
References
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Journal ArticleDOI
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
Randall Keichi Saiki,David H. Gelfand,Susanne Stoffel,Stephen J. Scharf,Russell Higuchi,Glenn Thomas Horn,Kary B. Mullis,Henry A. Erlich +7 more
TL;DR: A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified.
Book ChapterDOI
Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.
Kary B. Mullis,Fred A. Faloona +1 more
TL;DR: A method whereby a nucleic acid sequence can be exponentially amplified in vitro is described in the chapter, and the possibility of utilizing a heat-stable DNA polymerase is explored so as to avoid the need for addition of new enzyme after each cycle of thermal denaturation.
Journal ArticleDOI
DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells
Chin-Yih Ou,Shirley Kwok,Sheila W. Mitchell,David Henry Mack,John J. Sninsky,John W. Krebs,Paul M. Feorino,Donna T. Warfield,Gerald Schochetman +8 more
TL;DR: This method of DNA amplification made it possible to obtain results within 3 days, whereas virus isolation takes up to 3 to 4 weeks, and may therefore be used to complement or replace virus isolation as a routine means of determining HIV-1 infection.
Journal ArticleDOI
DNA typing from single hairs
TL;DR: Three different means of DNA typing are used for the determination of amplified DNA fragment length differences, hybridization with allele-specific oligonucleotide probes, and direct DNA sequencing on single human hairs to detect genetically variable mitochondrial and nuclear DNA sequences.
Journal ArticleDOI
Amplification and analysis of DNA sequences in single human sperm and diploid cells
Honghua Li,Ulf B. Gyllensten,Xiangfeng Cui,Randall Keichi Saiki,Henry A. Erlich,Norman Arnheim +5 more
TL;DR: The use of the polymerase chain reaction for analysing DNA sequences in individual diploid cells and human sperm shows that two genetic loci can be co-amplified from a single sperm, which may allow the analysis of previously inaccessible genetic phenomena.