Journal ArticleDOI
Avoiding false positives with PCR
S Kwok,Russell Higuchi +1 more
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TLDR
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment and adherence to a strict set of protocols can avoid disaster.Abstract:
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment. Tidiness and adherence to a strict set of protocols can avoid disaster.read more
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Journal Article
Mouse mammary tumor virus-like gene sequences in breast tumors of Australian and Vietnamese women.
TL;DR: MMTV-like gene sequences are found in only some human populations and are rarely found in normal human breast tissue from all populations, suggesting they are not present in the normal human genome and have been acquired.
Journal ArticleDOI
Development and clinical evaluation of a polymerase chain reaction test for detection of Chlamydia trachomatis.
J M Ossewaarde,M. Rieffe,Maja Rozenberg-Arska,P M Ossenkoppele,R. P. Nawrocki,A. M. van Loon +5 more
TL;DR: It is concluded that the PCR is the most sensitive technique for laboratory detection of C. trachomatis.
Journal ArticleDOI
Diagnostic value of detecting JC virus DNA in cerebrospinal fluid of patients with progressive multifocal leukoencephalopathy.
TL;DR: JC virus DNA was detected by PCR in the cerebrospinal fluid of 17 of 23 patients with confirmed cases of progressive multifocal leukoencephalopathy and 2 of 48 controls without progressive multifocally leukoencesphalopathy.
Journal ArticleDOI
Diagnosis of Streptococcus pneumoniae Lower Respiratory Infection in Hospitalized Children by Culture, Polymerase Chain Reaction, Serological Testing, and Urinary Antigen Detection
Ian C. Michelow,Juanita Lozano,Kurt Olsen,Collin S. Goto,Nancy K. Rollins,Faryal Ghaffar,Violeta Rodriguez-Cerrato,Maija Leinonen,George H. McCracken +8 more
TL;DR: Findings support the use of PCR tests to evaluate the protective efficacy of pneumococcal conjugate vaccines and to identify promptly children with pretreated or nonbacteremic pneumococCal lower respiratory infections.
Journal ArticleDOI
Use of the polymerase chain reaction to detect Legionella DNA in urine and serum samples from patients with pneumonia.
David R. Murdoch,E J Walford,Lance C. Jennings,G. J. Light,M. I. Schousboe,A. Y. Chereshsky,Stephen T. Chambers,G. I. Town +7 more
TL;DR: Use of polymerase chain reaction with primers that amplify a 104-base pair segment of the coding region of the 5S tRNA gene to detect Legionella DNA in urine and serum samples from patients with pneumonia shows promise for the rapid diagnosis of legionella pneumonia.
References
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Journal ArticleDOI
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
Randall Keichi Saiki,David H. Gelfand,Susanne Stoffel,Stephen J. Scharf,Russell Higuchi,Glenn Thomas Horn,Kary B. Mullis,Henry A. Erlich +7 more
TL;DR: A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified.
Book ChapterDOI
Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.
Kary B. Mullis,Fred A. Faloona +1 more
TL;DR: A method whereby a nucleic acid sequence can be exponentially amplified in vitro is described in the chapter, and the possibility of utilizing a heat-stable DNA polymerase is explored so as to avoid the need for addition of new enzyme after each cycle of thermal denaturation.
Journal ArticleDOI
DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells
Chin-Yih Ou,Shirley Kwok,Sheila W. Mitchell,David Henry Mack,John J. Sninsky,John W. Krebs,Paul M. Feorino,Donna T. Warfield,Gerald Schochetman +8 more
TL;DR: This method of DNA amplification made it possible to obtain results within 3 days, whereas virus isolation takes up to 3 to 4 weeks, and may therefore be used to complement or replace virus isolation as a routine means of determining HIV-1 infection.
Journal ArticleDOI
DNA typing from single hairs
TL;DR: Three different means of DNA typing are used for the determination of amplified DNA fragment length differences, hybridization with allele-specific oligonucleotide probes, and direct DNA sequencing on single human hairs to detect genetically variable mitochondrial and nuclear DNA sequences.
Journal ArticleDOI
Amplification and analysis of DNA sequences in single human sperm and diploid cells
Honghua Li,Ulf B. Gyllensten,Xiangfeng Cui,Randall Keichi Saiki,Henry A. Erlich,Norman Arnheim +5 more
TL;DR: The use of the polymerase chain reaction for analysing DNA sequences in individual diploid cells and human sperm shows that two genetic loci can be co-amplified from a single sperm, which may allow the analysis of previously inaccessible genetic phenomena.