Journal ArticleDOI
Avoiding false positives with PCR
S Kwok,Russell Higuchi +1 more
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TLDR
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment and adherence to a strict set of protocols can avoid disaster.Abstract:
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment. Tidiness and adherence to a strict set of protocols can avoid disaster.read more
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Book
Fundamentals of Forensic DNA Typing
TL;DR: The basic principles of Forensic DNA Typing and its applications in medicine, science and research are studied.
Multiplex detection of four pathogenic retroviruses using molecular beacons (real-time PCRyhairpin-forming probesymulticolor f luorescent labelsyhomogeneous assaysyviral quantitation)
Jacqueline A. M. Vet,Amit R. Majithia,Salvatore A. E. Marras,Sanjay Tyagi,Syamalima Dube,Bernard J. Poiesz,Fred R. Kramer +6 more
TL;DR: In this paper, a multiplex nucleic acid (MNA) assay was proposed to identify and determine the abundance of four different patho- genic retroviruses (HIV-1, HIV-2, and human T-lymphotrophic virus types I and II).
Journal ArticleDOI
Monitoring of Human Cytomegalovirus Infections and Ganciclovir Treatment in Heart Transplant Recipients by Determination of Viremia, Antigenemia, and DNAemia
G. Gerna,Donato Zipeto,M. Parea,Maria Grazia Revello,Enrico Silini,Elena Percivalle,Maurizio Zavattoni,Paolo Grossi,Gabriele Milanesi +8 more
TL;DR: Follow-up of HCMV infections in heart transplant recipients showed that PCR can detect viral appearance in blood 7-10 days earlier than assays for antigenemia/viremia, and viral disappearance from blood, as assessed by PCR, occurred weeks or months later than revealed by other assays.
Journal ArticleDOI
Detection of human herpesvirus 6 in plasma of children with primary infection and immunosuppressed patients by polymerase chain reaction.
Paola Secchiero,Donald R. Carrigan,Yoshizo Asano,Laura Benedetti,Richard W. Crowley,Anthony L. Komaroff,Robert C. Gallo,Paolo Lusso +7 more
TL;DR: A sensitive and specific polymerase chain reaction method for the detection of human herpesvirus 6 (HHV-6) DNA in serum or plasma has been developed and is a marker of active infection that can be used to investigate the role of HHV- 6 in human disease.
Journal ArticleDOI
Polymerase chain reaction detection of the BCR-ABL fusion transcript after allogeneic marrow transplantation for chronic myeloid leukemia: results and implications in 346 patients.
Jerry Radich,GB Gehly,Ted Gooley,Eileen Bryant,Reginald A. Clift,Steven J. Collins,S Edmands,J Kirk,A Lee,P Kessler +9 more
TL;DR: Analysis of patients after bone marrow transplantation for chronic myeloid leukemia for the presence of the bcr-abl transcript detected by the polymerase chain reaction (PCR) indicates that a PCR-positive result at 6 to 12 months post-BMT is an independent predictor of subsequent relapse which provides an opportunity for early therapeutic intervention.
References
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Journal ArticleDOI
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
Randall Keichi Saiki,David H. Gelfand,Susanne Stoffel,Stephen J. Scharf,Russell Higuchi,Glenn Thomas Horn,Kary B. Mullis,Henry A. Erlich +7 more
TL;DR: A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified.
Book ChapterDOI
Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.
Kary B. Mullis,Fred A. Faloona +1 more
TL;DR: A method whereby a nucleic acid sequence can be exponentially amplified in vitro is described in the chapter, and the possibility of utilizing a heat-stable DNA polymerase is explored so as to avoid the need for addition of new enzyme after each cycle of thermal denaturation.
Journal ArticleDOI
DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells
Chin-Yih Ou,Shirley Kwok,Sheila W. Mitchell,David Henry Mack,John J. Sninsky,John W. Krebs,Paul M. Feorino,Donna T. Warfield,Gerald Schochetman +8 more
TL;DR: This method of DNA amplification made it possible to obtain results within 3 days, whereas virus isolation takes up to 3 to 4 weeks, and may therefore be used to complement or replace virus isolation as a routine means of determining HIV-1 infection.
Journal ArticleDOI
DNA typing from single hairs
TL;DR: Three different means of DNA typing are used for the determination of amplified DNA fragment length differences, hybridization with allele-specific oligonucleotide probes, and direct DNA sequencing on single human hairs to detect genetically variable mitochondrial and nuclear DNA sequences.
Journal ArticleDOI
Amplification and analysis of DNA sequences in single human sperm and diploid cells
Honghua Li,Ulf B. Gyllensten,Xiangfeng Cui,Randall Keichi Saiki,Henry A. Erlich,Norman Arnheim +5 more
TL;DR: The use of the polymerase chain reaction for analysing DNA sequences in individual diploid cells and human sperm shows that two genetic loci can be co-amplified from a single sperm, which may allow the analysis of previously inaccessible genetic phenomena.