Journal ArticleDOI
Avoiding false positives with PCR
S Kwok,Russell Higuchi +1 more
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TLDR
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment and adherence to a strict set of protocols can avoid disaster.Abstract:
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment. Tidiness and adherence to a strict set of protocols can avoid disaster.read more
Citations
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Journal ArticleDOI
Rapid and quantitative detection of human adenovirus DNA by real-time PCR.
TL;DR: Real‐time PCR with consensus primer and probe sequences for a conserved region of the hexon gene is designed and evaluated and is found to be a sensitive and quantitative procedure for the detection of adenovirus infections.
Journal ArticleDOI
Adoptive immunotherapy evaluating escalating doses of donor leukocytes for relapse of chronic myeloid leukemia after bone marrow transplantation: separation of graft-versus-leukemia responses from graft-versus-host disease
Stephen Mackinnon,Esperanza B. Papadopoulos,MH Carabasi,L Reich,Nancy H. Collins,Farid Boulad,Hugo Castro-Malaspina,Barrett H. Childs,Alfred P. Gillio,Nancy A. Kernan +9 more
TL;DR: The dose of donor leukocytes or T cells used may be important in determining both the GVL response and the incidence of GVHD, and 15 of the 17 evaluable patients have become BCR-ABL negative by PCR.
Journal ArticleDOI
Prenatal diagnosis of fetal RhD status by molecular analysis of maternal plasma.
Yuk-Ming Dennis Lo,N. M. Hjelm,Carrie Fidler,Ian L. Sargent,Michael F. Murphy,Paul Chamberlain,Priscilla M.K. Poon,Christopher W.G. Redman,J. S. Wainscoat +8 more
TL;DR: Noninvasive fetal RhD genotyping can be performed rapidly and reliably with the use of maternal plasma beginning in the second trimester of pregnancy.
Journal ArticleDOI
Immunologic purging of marrow assessed by PCR before autologous bone marrow transplantation for B-cell lymphoma.
John G. Gribben,Arnold S. Freedman,Donna Neuberg,Denis-Claude Roy,K Blake,S D Woo,Michael L. Grossbard,SN Rabinowe,F Coral,Gordon J. Freeman +9 more
TL;DR: Evidence is provided of the clinical usefulness of ex vivo purging of autologous bone marrow in the treatment of patients with lymphoma and suggest that the reinfusion of malignant cells in autOLOGous marrow contributes to relapse.
Journal ArticleDOI
Quantitative analysis of MDR1 (multidrug resistance) gene expression in human tumors by polymerase chain reaction.
Kevin E. Noonan,C. Beck,T. A. Holzmayer,Janice E. Chin,Jay S. Wunder,Irene L. Andrulis,Adi F. Gazdar,Cheryl L. Willman,B. Griffith,D. D. Von Hoff,Igor B. Roninson +10 more
TL;DR: A highly sensitive, specific, and quantitative protocol for measuring the levels of MDR1 mRNA in clinical samples, based on the polymerase chain reaction is devised, which was found to be effective in most solid tumors and leukemias tested.
References
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Journal ArticleDOI
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
Randall Keichi Saiki,David H. Gelfand,Susanne Stoffel,Stephen J. Scharf,Russell Higuchi,Glenn Thomas Horn,Kary B. Mullis,Henry A. Erlich +7 more
TL;DR: A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified.
Book ChapterDOI
Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.
Kary B. Mullis,Fred A. Faloona +1 more
TL;DR: A method whereby a nucleic acid sequence can be exponentially amplified in vitro is described in the chapter, and the possibility of utilizing a heat-stable DNA polymerase is explored so as to avoid the need for addition of new enzyme after each cycle of thermal denaturation.
Journal ArticleDOI
DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells
Chin-Yih Ou,Shirley Kwok,Sheila W. Mitchell,David Henry Mack,John J. Sninsky,John W. Krebs,Paul M. Feorino,Donna T. Warfield,Gerald Schochetman +8 more
TL;DR: This method of DNA amplification made it possible to obtain results within 3 days, whereas virus isolation takes up to 3 to 4 weeks, and may therefore be used to complement or replace virus isolation as a routine means of determining HIV-1 infection.
Journal ArticleDOI
DNA typing from single hairs
TL;DR: Three different means of DNA typing are used for the determination of amplified DNA fragment length differences, hybridization with allele-specific oligonucleotide probes, and direct DNA sequencing on single human hairs to detect genetically variable mitochondrial and nuclear DNA sequences.
Journal ArticleDOI
Amplification and analysis of DNA sequences in single human sperm and diploid cells
Honghua Li,Ulf B. Gyllensten,Xiangfeng Cui,Randall Keichi Saiki,Henry A. Erlich,Norman Arnheim +5 more
TL;DR: The use of the polymerase chain reaction for analysing DNA sequences in individual diploid cells and human sperm shows that two genetic loci can be co-amplified from a single sperm, which may allow the analysis of previously inaccessible genetic phenomena.