Journal ArticleDOI
Avoiding false positives with PCR
S Kwok,Russell Higuchi +1 more
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TLDR
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment and adherence to a strict set of protocols can avoid disaster.Abstract:
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment. Tidiness and adherence to a strict set of protocols can avoid disaster.read more
Citations
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Diagnosis of Cat Scratch Disease with Detection of Bartonella henselae by PCR: a Study of Patients with Lymph Node Enlargement
Yves Hansmann,Sylvie DeMartino,Yves Piémont,Nicolas Meyer,Philippe Mariet,R. Heller,Daniel Christmann,Benoît Jaulhac +7 more
TL;DR: A study was performed to determine the diagnostic value of PCR analysis for the detection of B. henselae for the diagnosis of CSD and its place in the diagnostic strategy alongside the classical criteria.
Journal ArticleDOI
Hepatitis C Virus Replicates in the Liver of Patients Who Have a Sustained Response to Antiviral Treatment
Inmaculada Castillo,Elena Rodríguez-Iñigo,Juan Manuel López-Alcorocho,Margarita Pardo,Javier Bartolomé,Vicente Carreño +5 more
TL;DR: HCV persisted and replicated in the livers and peripheral blood mononuclear cells of most sustained responders, and these patients did not experience HCV infection clearance, despite apparent clinical disease resolution.
Journal ArticleDOI
"Cytomegalovirus disease" in renal allograft recipients: Is human herpesvirus 7 a Co-factor for disease progression?
TL;DR: It is concluded that in patients with CMV DNAemia, concurrent infection/reactivation of HHV7 (and possibly HHV6) is associated with an increased risk of progression to “CMV disease.”
Journal ArticleDOI
Detection of HIV-1 gene sequences in hippocampal neurons isolated from postmortem AIDS brains by laser capture microdissection.
Jorge Torres-Munoz,Patricia S. Stockton,Noe Tacoronte,Brenda Roberts,Robert R. Maronpot,Carol K. Petito +5 more
TL;DR: The results indicate that brain neurons in vivo contain HIV-1 DNA sequences consistent with latent infection by this virus, and suggest that neurons display a selective vulnerability for HIV infection.
Journal ArticleDOI
Evaluation of the range of clinical presentations of herpes simplex encephalitis by using polymerase chain reaction assay of cerebrospinal fluid samples.
Renan Barros Domingues,Ana Maria Crous Tsanaclis,Cláudio Sérgio Pannuti,Matthew S. Mayo,Fred D. Lakeman +4 more
TL;DR: In this study, PCR assay of CSF samples obtained from 29 patients with focal, mild, and diffuse encephalitis, respectively, was performed and revealed that atypical and less severe forms ofEncephalitis are caused by herpes simplex virus.
References
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Journal ArticleDOI
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
Randall Keichi Saiki,David H. Gelfand,Susanne Stoffel,Stephen J. Scharf,Russell Higuchi,Glenn Thomas Horn,Kary B. Mullis,Henry A. Erlich +7 more
TL;DR: A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified.
Book ChapterDOI
Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.
Kary B. Mullis,Fred A. Faloona +1 more
TL;DR: A method whereby a nucleic acid sequence can be exponentially amplified in vitro is described in the chapter, and the possibility of utilizing a heat-stable DNA polymerase is explored so as to avoid the need for addition of new enzyme after each cycle of thermal denaturation.
Journal ArticleDOI
DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells
Chin-Yih Ou,Shirley Kwok,Sheila W. Mitchell,David Henry Mack,John J. Sninsky,John W. Krebs,Paul M. Feorino,Donna T. Warfield,Gerald Schochetman +8 more
TL;DR: This method of DNA amplification made it possible to obtain results within 3 days, whereas virus isolation takes up to 3 to 4 weeks, and may therefore be used to complement or replace virus isolation as a routine means of determining HIV-1 infection.
Journal ArticleDOI
DNA typing from single hairs
TL;DR: Three different means of DNA typing are used for the determination of amplified DNA fragment length differences, hybridization with allele-specific oligonucleotide probes, and direct DNA sequencing on single human hairs to detect genetically variable mitochondrial and nuclear DNA sequences.
Journal ArticleDOI
Amplification and analysis of DNA sequences in single human sperm and diploid cells
Honghua Li,Ulf B. Gyllensten,Xiangfeng Cui,Randall Keichi Saiki,Henry A. Erlich,Norman Arnheim +5 more
TL;DR: The use of the polymerase chain reaction for analysing DNA sequences in individual diploid cells and human sperm shows that two genetic loci can be co-amplified from a single sperm, which may allow the analysis of previously inaccessible genetic phenomena.