Journal ArticleDOI
Avoiding false positives with PCR
S Kwok,Russell Higuchi +1 more
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TLDR
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment and adherence to a strict set of protocols can avoid disaster.Abstract:
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment. Tidiness and adherence to a strict set of protocols can avoid disaster.read more
Citations
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Rapid routine detection of enterovirus RNA in cerebrospinal fluid by a one-step real-time RT-PCR assay
TL;DR: The new TM-PCR is a convincing alternative to conventional PCR protocols for the diagnosis of enterovirus meningitis because the one-step strategy limits hands on time and cross contamination risk combined with accelerated assay procedure of only 100 min.
Journal ArticleDOI
High frequency of human papillomavirus infection in carcinoma of the urinary bladder.
TL;DR: The prevalence of type 6, 11, 16, 18, and 33 human papillomavirus (HPV) was investigated with the polymerase chain reaction (PCR) on formalin‐fixed, paraffin wax‐embedded material, including 48 neoplastic and 21 normal urinary bladder specimens.
Journal ArticleDOI
Molecular evidence of organ-related transmission of Kaposi sarcoma-associated herpesvirus or human herpesvirus-8 in transplant patients.
Mario Luppi,P. Barozzi,P. Barozzi,Gaia Santagostino,Gaia Santagostino,Raffaella Trovato,Raffaella Trovato,Thomas F. Schulz,Thomas F. Schulz,Roberto Marasca,Roberto Marasca,Davide Bottalico,Davide Bottalico,Lucia Bignardi,Lucia Bignardi,Giuseppe Torelli,Giuseppe Torelli +16 more
TL;DR: The 100% identity of nucleotide sequence of the most variable viral region and the presence of the same, single orf -73 type in both patients provides strong molecular evidence of organ-related transmission of HHV-8 in the setting of transplantation.
Journal ArticleDOI
Diagnostic Value of Immunoglobulin G (IgG) and IgM Anti-Hepatitis E Virus (HEV) Tests Based on HEV RNA in an Area Where Hepatitis E Is Not Endemic
Chen-Chun Lin,Jaw Ching Wu,Ting-Tsung Chang,Wen-Yu Chang,Ming-Lung Yu,Albert W. Tam,Shen-Chin Wang,Yi Hsiang Huang,Yi Hsiang Huang,Full-Young Chang,Full-Young Chang,Shou-Dong Lee,Shou-Dong Lee +12 more
TL;DR: IgG anti- HEV is a good diagnostic test for screening for AHE in nonendemic areas and the high rate of prevalence of anti-HEV in healthy subjects indicates that subclinical infection may exist.
Journal ArticleDOI
The predominance of hepatitis delta virus genotype I among chronically infected Italian patients.
Grazia Anna Niro,Antonina Smedile,Angelo Andriulli,Mario Rizzetto,John L. Gerin,John L. Casey +5 more
TL;DR: It is concluded that in Italy, where HDV infection has been endemic, the overwhelmingly predominant genotype of HDV is genotype I, and this genotype appeared to be associated with a broad spectrum of chronic disease.
References
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Journal ArticleDOI
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
Randall Keichi Saiki,David H. Gelfand,Susanne Stoffel,Stephen J. Scharf,Russell Higuchi,Glenn Thomas Horn,Kary B. Mullis,Henry A. Erlich +7 more
TL;DR: A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified.
Book ChapterDOI
Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.
Kary B. Mullis,Fred A. Faloona +1 more
TL;DR: A method whereby a nucleic acid sequence can be exponentially amplified in vitro is described in the chapter, and the possibility of utilizing a heat-stable DNA polymerase is explored so as to avoid the need for addition of new enzyme after each cycle of thermal denaturation.
Journal ArticleDOI
DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells
Chin-Yih Ou,Shirley Kwok,Sheila W. Mitchell,David Henry Mack,John J. Sninsky,John W. Krebs,Paul M. Feorino,Donna T. Warfield,Gerald Schochetman +8 more
TL;DR: This method of DNA amplification made it possible to obtain results within 3 days, whereas virus isolation takes up to 3 to 4 weeks, and may therefore be used to complement or replace virus isolation as a routine means of determining HIV-1 infection.
Journal ArticleDOI
DNA typing from single hairs
TL;DR: Three different means of DNA typing are used for the determination of amplified DNA fragment length differences, hybridization with allele-specific oligonucleotide probes, and direct DNA sequencing on single human hairs to detect genetically variable mitochondrial and nuclear DNA sequences.
Journal ArticleDOI
Amplification and analysis of DNA sequences in single human sperm and diploid cells
Honghua Li,Ulf B. Gyllensten,Xiangfeng Cui,Randall Keichi Saiki,Henry A. Erlich,Norman Arnheim +5 more
TL;DR: The use of the polymerase chain reaction for analysing DNA sequences in individual diploid cells and human sperm shows that two genetic loci can be co-amplified from a single sperm, which may allow the analysis of previously inaccessible genetic phenomena.