Journal ArticleDOI
Avoiding false positives with PCR
S Kwok,Russell Higuchi +1 more
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TLDR
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment and adherence to a strict set of protocols can avoid disaster.Abstract:
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment. Tidiness and adherence to a strict set of protocols can avoid disaster.read more
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Expression of somatostatin receptor types 2, 3 and 5 in biopsies and surgical specimens of human lung tumours. Correlation with preoperative octreotide scintigraphy.
Mauro Papotti,Sabrina Croce,Marilena Bellò,Massimo Bongiovanni,Elena Allia,Marcus Schindler,Gianni Bussolati +6 more
TL;DR: Octreoscan findings and sst expression were correlated in 22/25 cases, thus indicating that a cell membrane sst immunoreactivity in a biopsy reliably predicts the tumor–receptor profile before its resection.
Journal ArticleDOI
Detection of neuroblastoma cells in bone marrow and peripheral blood at diagnosis by the reverse transcriptase‐polymerase chain reaction for tyrosine hydroxylase mRNA
TL;DR: The feasibility and clinical value of using the reverse transcriptase‐ (RT) polymerase chain reaction (PCR) to amplify mRNA for tyrosine hydroxylase (TH), the first enzyme of catecholamine synthesis, was evaluated to detect neuroblastoma cells in patient samples.
Journal ArticleDOI
Prognostic significance of WT1 gene expression at diagnosis in adult de novo acute myeloid leukemia
D Schmid,Georg Heinze,B Linnerth,K Tisljar,Rajko Kusec,Klaus Geissler,C Sillaber,Klaus Laczika,Margit Mitterbauer,S Zöchbauer,Christine Mannhalter,Oskar A. Haas,Klaus Lechner,Ulrich Jäger,A. Gaiger +14 more
TL;DR: WT1 gene expression at diagnosis is not associated with specific characteristics of AML blast cells and is not a prognostic factor for CR, remission duration and overall survival in acute myeloid leukemia.
Journal ArticleDOI
Occult Hepatitis C Virus Infection among Hemodialysis Patients
Guillermina Barril,Inmaculada Castillo,María Dolores Arenas,Mario Espinosa,Juan Garcia-Valdecasas,Nuria Garcia-Fernandez,Emilio González-Parra,Jose M. Alcazar,Carmen María Martínez Sánchez,José Carlos Diez-Baylón,Pilar Balanza Martínez,Javier Bartolomé,Vicente Carreño +12 more
TL;DR: The prevalence of occultHCV infection is high among hemodialysis patients with persistently abnormal values of liver enzymes of unknown cause, and the clinical significance of occult HCV infection in these patients requires further study.
Journal ArticleDOI
Viral and host factors that contribute to efficacy of interferon-alpha 2a therapy in patients with chronic hepatitis C.
TL;DR: It is indicated that HCV genotype, HAI score, and schedule of administration can be important predictors of the response to interferon therapy.
References
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Journal ArticleDOI
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
Randall Keichi Saiki,David H. Gelfand,Susanne Stoffel,Stephen J. Scharf,Russell Higuchi,Glenn Thomas Horn,Kary B. Mullis,Henry A. Erlich +7 more
TL;DR: A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified.
Book ChapterDOI
Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.
Kary B. Mullis,Fred A. Faloona +1 more
TL;DR: A method whereby a nucleic acid sequence can be exponentially amplified in vitro is described in the chapter, and the possibility of utilizing a heat-stable DNA polymerase is explored so as to avoid the need for addition of new enzyme after each cycle of thermal denaturation.
Journal ArticleDOI
DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells
Chin-Yih Ou,Shirley Kwok,Sheila W. Mitchell,David Henry Mack,John J. Sninsky,John W. Krebs,Paul M. Feorino,Donna T. Warfield,Gerald Schochetman +8 more
TL;DR: This method of DNA amplification made it possible to obtain results within 3 days, whereas virus isolation takes up to 3 to 4 weeks, and may therefore be used to complement or replace virus isolation as a routine means of determining HIV-1 infection.
Journal ArticleDOI
DNA typing from single hairs
TL;DR: Three different means of DNA typing are used for the determination of amplified DNA fragment length differences, hybridization with allele-specific oligonucleotide probes, and direct DNA sequencing on single human hairs to detect genetically variable mitochondrial and nuclear DNA sequences.
Journal ArticleDOI
Amplification and analysis of DNA sequences in single human sperm and diploid cells
Honghua Li,Ulf B. Gyllensten,Xiangfeng Cui,Randall Keichi Saiki,Henry A. Erlich,Norman Arnheim +5 more
TL;DR: The use of the polymerase chain reaction for analysing DNA sequences in individual diploid cells and human sperm shows that two genetic loci can be co-amplified from a single sperm, which may allow the analysis of previously inaccessible genetic phenomena.