Journal ArticleDOI
Avoiding false positives with PCR
S Kwok,Russell Higuchi +1 more
Reads0
Chats0
TLDR
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment and adherence to a strict set of protocols can avoid disaster.Abstract:
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment. Tidiness and adherence to a strict set of protocols can avoid disaster.read more
Citations
More filters
Journal ArticleDOI
Evaluation of mixed chimerism by in vitro amplification of dinucleotide repeat sequences using the polymerase chain reaction
TL;DR: A highly sensitive novel method based on the polymerase chain reaction (PCR) was reported to assess mixed hematopoietic chimerism after allogeneic bone marrow transplantation, finding sudden increases in the proportions of recipient cells may be a prelude to graft rejection or relapse.
Journal ArticleDOI
Detection of Viral DNA in Neonatal Herpes Simplex Virus Infections: Frequent and Prolonged Presence in Serum and Cerebrospinal Fluid
Hiroshi Kimura,Masahide Futamura,Hideyuki Kito,Tseunesaburo Ando,Masahiko Goto,Kiyotaka Kuzushima,Motohiro Shibata,Tsuneo Morishima +7 more
TL;DR: PCR assay results suggest that HSV may be spread principally via viremia in neonates, especially in culture-negative cases of neonatal HSV infections.
Journal ArticleDOI
An AML1/ETO fusion transcript is consistently detected by RNA-based polymerase chain reaction in acute myelogenous leukemia containing the (8;21)(q22;q22) translocation
James R. Downing,David R. Head,A. M. Curcio-Brint,M. G. Hulshof,T. A. Motroni,Susana C. Raimondi,Andrew J. Carroll,Harry A. Drabkin,C. L. Willman,Karl S. Theil,Curt I. Civin,P. Erickson +11 more
TL;DR: Studies on the sensitivity and specificity of this approach show that PCR analysis can be used as a rapid, accurate, and sensitive means for detecting this chromosomal abnormality, and for following the patients' response to therapy.
Journal ArticleDOI
Rapid HLA-DPB typing using enzymatically amplified DNA and nonradioactive sequence-specific oligonucleotide probes.
TL;DR: A simple and rapid method for characterizing the polymorphism at the HLA-DPB1 locus has been developed and the application of this technology to the fields of tissue typing and individual identity is discussed.
Journal ArticleDOI
Rapid Identification of Candida Species with Species-Specific DNA Probes
TL;DR: This method is simple, rapid, and feasible for identifying Candida species in clinical laboratories that utilize molecular identification techniques and provides a novel method to differentiate the new species, C. dubliniensis, from C. albicans.
References
More filters
Journal ArticleDOI
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
Randall Keichi Saiki,David H. Gelfand,Susanne Stoffel,Stephen J. Scharf,Russell Higuchi,Glenn Thomas Horn,Kary B. Mullis,Henry A. Erlich +7 more
TL;DR: A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified.
Book ChapterDOI
Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.
Kary B. Mullis,Fred A. Faloona +1 more
TL;DR: A method whereby a nucleic acid sequence can be exponentially amplified in vitro is described in the chapter, and the possibility of utilizing a heat-stable DNA polymerase is explored so as to avoid the need for addition of new enzyme after each cycle of thermal denaturation.
Journal ArticleDOI
DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells
Chin-Yih Ou,Shirley Kwok,Sheila W. Mitchell,David Henry Mack,John J. Sninsky,John W. Krebs,Paul M. Feorino,Donna T. Warfield,Gerald Schochetman +8 more
TL;DR: This method of DNA amplification made it possible to obtain results within 3 days, whereas virus isolation takes up to 3 to 4 weeks, and may therefore be used to complement or replace virus isolation as a routine means of determining HIV-1 infection.
Journal ArticleDOI
DNA typing from single hairs
TL;DR: Three different means of DNA typing are used for the determination of amplified DNA fragment length differences, hybridization with allele-specific oligonucleotide probes, and direct DNA sequencing on single human hairs to detect genetically variable mitochondrial and nuclear DNA sequences.
Journal ArticleDOI
Amplification and analysis of DNA sequences in single human sperm and diploid cells
Honghua Li,Ulf B. Gyllensten,Xiangfeng Cui,Randall Keichi Saiki,Henry A. Erlich,Norman Arnheim +5 more
TL;DR: The use of the polymerase chain reaction for analysing DNA sequences in individual diploid cells and human sperm shows that two genetic loci can be co-amplified from a single sperm, which may allow the analysis of previously inaccessible genetic phenomena.