Journal ArticleDOI
Avoiding false positives with PCR
S Kwok,Russell Higuchi +1 more
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TLDR
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment and adherence to a strict set of protocols can avoid disaster.Abstract:
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment. Tidiness and adherence to a strict set of protocols can avoid disaster.read more
Citations
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Journal ArticleDOI
Molecular identification by "suicide PCR" of Yersinia pestis as the agent of medieval black death.
Didier Raoult,Gérard Aboudharam,Eric Crubézy,Georges Larrouy,Bertrand Ludes,Michel Drancourt +5 more
TL;DR: Medieval Black Death was plague and the causative organism was debated because no definitive evidence has been obtained to confirm the role of Yersinia pestis as the agent of plague, according to the new "suicide PCR" protocol in which the primers are used only once.
Journal ArticleDOI
Association of Amino Acid Substitution Pattern in Core Protein of Hepatitis C Virus Genotype 1b High Viral Load and Non-Virological Response to Interferon-Ribavirin Combination Therapy
Norio Akuta,Fumitaka Suzuki,Hitomi Sezaki,Yoshiyuki Suzuki,Tetsuya Hosaka,Takashi Someya,Masahiro Kobayashi,Satoshi Saitoh,Sachiyo Watahiki,Junko Sato,Marie Matsuda,Mariko Kobayashi,Yasuji Arase,Kenji Ikeda,Hiromitsu Kumada +14 more
TL;DR: The results suggest that serum albumin and amino acid substitution patterns in the core region in patients with high titers of HCV genotype 1b may have an effect on combination therapy in NVRs.
Journal ArticleDOI
Predictive factors of early and sustained responses to peginterferon plus ribavirin combination therapy in Japanese patients infected with hepatitis C virus genotype 1b: Amino acid substitutions in the core region and low-density lipoprotein cholesterol levels
Norio Akuta,Fumitaka Suzuki,Yusuke Kawamura,Hiromi Yatsuji,Hitomi Sezaki,Yoshiyuki Suzuki,Tetsuya Hosaka,Masahiro Kobayashi,Mariko Kobayashi,Yasuji Arase,Kenji Ikeda,Hiromitsu Kumada +11 more
TL;DR: This study identified aa substitutions in the core region and serum LDL-C as predictors of response to PEG-IFN-RBV therapy in Japanese patients infected with HCV genotype 1b.
Journal ArticleDOI
Heminested PCR assay for detection of six genotypes of rabies and rabies-related viruses.
TL;DR: A heminested reverse transcriptase PCR (hnRT-PCR) protocol which is rapid and sensitive for the detection of rabies virus and rabies-related viruses is described and a comparison of the sensitivity of the standard fluorescent-antibody test (FAT) for rabies antigen and that of hnRT- PCR for rabie viral RNA with degraded tissue infected with a genotype 1 virus is reported.
Patent
Microfluidic device for cell separation and uses thereof
TL;DR: In this paper, a mixture of cells is introduced into a microfluidic channel that selectively allows the passage of a desired type of cell, and the population of cells enriched in the desired type is then introduced into another micro fluidic channel to produce a population of additional cells enriched with the same properties, i.e., size, shape, deformability, surface characteristics, or intracellular properties.
References
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Journal ArticleDOI
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
Randall Keichi Saiki,David H. Gelfand,Susanne Stoffel,Stephen J. Scharf,Russell Higuchi,Glenn Thomas Horn,Kary B. Mullis,Henry A. Erlich +7 more
TL;DR: A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified.
Book ChapterDOI
Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.
Kary B. Mullis,Fred A. Faloona +1 more
TL;DR: A method whereby a nucleic acid sequence can be exponentially amplified in vitro is described in the chapter, and the possibility of utilizing a heat-stable DNA polymerase is explored so as to avoid the need for addition of new enzyme after each cycle of thermal denaturation.
Journal ArticleDOI
DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells
Chin-Yih Ou,Shirley Kwok,Sheila W. Mitchell,David Henry Mack,John J. Sninsky,John W. Krebs,Paul M. Feorino,Donna T. Warfield,Gerald Schochetman +8 more
TL;DR: This method of DNA amplification made it possible to obtain results within 3 days, whereas virus isolation takes up to 3 to 4 weeks, and may therefore be used to complement or replace virus isolation as a routine means of determining HIV-1 infection.
Journal ArticleDOI
DNA typing from single hairs
TL;DR: Three different means of DNA typing are used for the determination of amplified DNA fragment length differences, hybridization with allele-specific oligonucleotide probes, and direct DNA sequencing on single human hairs to detect genetically variable mitochondrial and nuclear DNA sequences.
Journal ArticleDOI
Amplification and analysis of DNA sequences in single human sperm and diploid cells
Honghua Li,Ulf B. Gyllensten,Xiangfeng Cui,Randall Keichi Saiki,Henry A. Erlich,Norman Arnheim +5 more
TL;DR: The use of the polymerase chain reaction for analysing DNA sequences in individual diploid cells and human sperm shows that two genetic loci can be co-amplified from a single sperm, which may allow the analysis of previously inaccessible genetic phenomena.