Journal ArticleDOI
Avoiding false positives with PCR
S Kwok,Russell Higuchi +1 more
Reads0
Chats0
TLDR
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment and adherence to a strict set of protocols can avoid disaster.Abstract:
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment. Tidiness and adherence to a strict set of protocols can avoid disaster.read more
Citations
More filters
Journal Article
Evaluation of six methods for extraction and purification of viral DNA from urine and serum samples.
Massimiliano Bergallo,Cristina Costa,Giorgio Gribaudo,Sonia Tarallo,Sara Baro,Alessandro Negro Ponzi,Rossana Cavallo +6 more
TL;DR: The presence of inhibitors was overcome by all DNA extraction techniques in urine samples, as evidenced by semiquantitative PCR amplification, and the most effective extraction methods were the phenol/chloroform technique and the silica gel extraction procedure for urine and serum samples, respectively.
Journal ArticleDOI
Sensitive and specific polymerase chain reaction assays for diagnosis of human T-cell lymphotropic virus type I (HTLV-I) and HTLV-II infections in HTLV-I/II-seropositive individuals.
TL;DR: To confirm and differentiate between human T-cell lymphotropic virus type I (HTLV-I) and HTLV-II infections, polymerase chain reaction samples of peripheral blood lymphocytes from 98 individuals seropositive for HT LV/II were analyzed using pol (SK110/111) and tax (SK43/44) consensus primer pairs.
Journal ArticleDOI
Life-Threatening Parvovirus B19-Associated Myocarditis and Cardiac Transplantation as Possible Therapy: Two Case Reports
G. Enders,J. Dötsch,J. Bauer,W. Nützenadel,H. Hengel,D. Haffner,Gunnar Schalasta,Karen Searle,K. E. Brown +8 more
TL;DR: Clinicians should be alerted to the possible role of parvovirus B19 in myocarditis presenting in immunocompetent patients, which was identified that resulted in life-threateningMyocarditis shortly after acute infection in immune-competent individuals.
Journal ArticleDOI
Hepatitis C viraemia in adults with type 2 autoimmune hepatitis.
Jeremy A. Garson,Marco Lenzi,Christopher J. Ring,Fabio Cassani,Giorgio Ballardini,M. Briggs,Richard S. Tedder,F.B. Bianchi +7 more
TL;DR: Neither anti‐HCV nor serum HCV RNA were detected in any of 6 controls with primary biliary cirrhosis or in 39 healthy blood donors, suggesting a role for HCV in the pathogenesis of type 2 autoimmune hepatitis.
Journal ArticleDOI
Serum levels of hepatitis C virus core protein in patients with chronic hepatitis C treated with interferon alfa
Eiji Tanaka,Kendo Kiyosawa,Akihiro Matsumoto,T Kashiwakuma,A Hasegawa,H Mori,O Yanagihara,Yohsuke Ohta +7 more
TL;DR: To assess the utility of measurements of serum HCV core protein during the course of treatment of chronic hepatitis C, 27 patients who were treated with a single schedule of interferon alfa (IFN-alpha) were studied.
References
More filters
Journal ArticleDOI
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
Randall Keichi Saiki,David H. Gelfand,Susanne Stoffel,Stephen J. Scharf,Russell Higuchi,Glenn Thomas Horn,Kary B. Mullis,Henry A. Erlich +7 more
TL;DR: A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified.
Book ChapterDOI
Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.
Kary B. Mullis,Fred A. Faloona +1 more
TL;DR: A method whereby a nucleic acid sequence can be exponentially amplified in vitro is described in the chapter, and the possibility of utilizing a heat-stable DNA polymerase is explored so as to avoid the need for addition of new enzyme after each cycle of thermal denaturation.
Journal ArticleDOI
DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells
Chin-Yih Ou,Shirley Kwok,Sheila W. Mitchell,David Henry Mack,John J. Sninsky,John W. Krebs,Paul M. Feorino,Donna T. Warfield,Gerald Schochetman +8 more
TL;DR: This method of DNA amplification made it possible to obtain results within 3 days, whereas virus isolation takes up to 3 to 4 weeks, and may therefore be used to complement or replace virus isolation as a routine means of determining HIV-1 infection.
Journal ArticleDOI
DNA typing from single hairs
TL;DR: Three different means of DNA typing are used for the determination of amplified DNA fragment length differences, hybridization with allele-specific oligonucleotide probes, and direct DNA sequencing on single human hairs to detect genetically variable mitochondrial and nuclear DNA sequences.
Journal ArticleDOI
Amplification and analysis of DNA sequences in single human sperm and diploid cells
Honghua Li,Ulf B. Gyllensten,Xiangfeng Cui,Randall Keichi Saiki,Henry A. Erlich,Norman Arnheim +5 more
TL;DR: The use of the polymerase chain reaction for analysing DNA sequences in individual diploid cells and human sperm shows that two genetic loci can be co-amplified from a single sperm, which may allow the analysis of previously inaccessible genetic phenomena.