Journal ArticleDOI
Avoiding false positives with PCR
S Kwok,Russell Higuchi +1 more
Reads0
Chats0
TLDR
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment and adherence to a strict set of protocols can avoid disaster.Abstract:
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment. Tidiness and adherence to a strict set of protocols can avoid disaster.read more
Citations
More filters
A Guide to HIV-1 Reverse Transcriptase and Protease Sequencing for Drug Resistance Studies.
TL;DR: The use of HIV-1 genotypic resistance testing in research settings to learn about the mechanisms and clinical significance of drug resistance and in clinical settings where it is used to help guide anti-HIV treatment are detailed.
FULL OF SOUND AND FURY: The Recent History of Ancient DNA
TL;DR: The discovery that DNA survives in ancient remains and can be amplified by the polymerase chain reaction has added a direct temporal dimension to evolutionary studies, and the evolutionary questions that can be addressed are far reaching.
Journal ArticleDOI
Detection of minimal residual disease in acute myelomonocytic leukemia with abnormal marrow eosinophils by nested polymerase chain reaction with allele specific amplification
TL;DR: It is confirmed that CBFB/MYH11 transcripts (with a predominant type A form) are present in most cases of inv(16) AML, and NPASA provides a useful and sensitive tool for the detection of minimal residual disease in inv( 16)AML and, potentially, in other leukemias associated with translocations that result in a predominant fusion transcript.
Journal ArticleDOI
Presence of fetal DNA in maternal plasma decades after pregnancy.
Pietro Invernizzi,Maria Luisa Biondi,Pier Maria Battezzati,Francesca Perego,Carlo Selmi,Federica Cecchini,Mauro Podda,Giuseppe Simoni +7 more
TL;DR: Not only fetal cells, but also fragments of fetal DNA can be present in the maternal circulation indefinitely after pregnancy, and this finding has practical implications for non-invasive prenatal diagnoses based on maternal blood, and may be considered for possible pathophysiological correlations.
Journal ArticleDOI
Use of filter paper (FTA®) technology for sampling, recovery and molecular characterisation of rabies viruses
TL;DR: The stability of viral RNA and the inactivation of infectivity make the FTA technology useful for the storage, transport, collection and subsequent molecular analysis of viral rabies RNA, facilitating epidemiological investigations in the field.
References
More filters
Journal ArticleDOI
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
Randall Keichi Saiki,David H. Gelfand,Susanne Stoffel,Stephen J. Scharf,Russell Higuchi,Glenn Thomas Horn,Kary B. Mullis,Henry A. Erlich +7 more
TL;DR: A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified.
Book ChapterDOI
Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.
Kary B. Mullis,Fred A. Faloona +1 more
TL;DR: A method whereby a nucleic acid sequence can be exponentially amplified in vitro is described in the chapter, and the possibility of utilizing a heat-stable DNA polymerase is explored so as to avoid the need for addition of new enzyme after each cycle of thermal denaturation.
Journal ArticleDOI
DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells
Chin-Yih Ou,Shirley Kwok,Sheila W. Mitchell,David Henry Mack,John J. Sninsky,John W. Krebs,Paul M. Feorino,Donna T. Warfield,Gerald Schochetman +8 more
TL;DR: This method of DNA amplification made it possible to obtain results within 3 days, whereas virus isolation takes up to 3 to 4 weeks, and may therefore be used to complement or replace virus isolation as a routine means of determining HIV-1 infection.
Journal ArticleDOI
DNA typing from single hairs
TL;DR: Three different means of DNA typing are used for the determination of amplified DNA fragment length differences, hybridization with allele-specific oligonucleotide probes, and direct DNA sequencing on single human hairs to detect genetically variable mitochondrial and nuclear DNA sequences.
Journal ArticleDOI
Amplification and analysis of DNA sequences in single human sperm and diploid cells
Honghua Li,Ulf B. Gyllensten,Xiangfeng Cui,Randall Keichi Saiki,Henry A. Erlich,Norman Arnheim +5 more
TL;DR: The use of the polymerase chain reaction for analysing DNA sequences in individual diploid cells and human sperm shows that two genetic loci can be co-amplified from a single sperm, which may allow the analysis of previously inaccessible genetic phenomena.