Journal ArticleDOI
Avoiding false positives with PCR
S Kwok,Russell Higuchi +1 more
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TLDR
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment and adherence to a strict set of protocols can avoid disaster.Abstract:
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment. Tidiness and adherence to a strict set of protocols can avoid disaster.read more
Citations
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Bacteriological and PCR analysis of clinical material aspirated from otitis media with effusions.
TL;DR: It is demonstrated that effusion fluid from otitis media cases contain a battery of bacterial species and these bacteria might play roles in the pathogens of OME.
Journal ArticleDOI
A multicenter randomized controlled trial of recombinant interferon‐α2b in patients with acute transfusion‐associated hepatitis C
Pietro Lampertico,Maria Grazia Rumi,Raffaella Romeo,Antonio Craxì,Roberta Soffredini,Daniela Biassoni,Massimo Colombo +6 more
TL;DR: One major finding was that short‐term treatment with interferon‐α was effective in most patients with acute hepatitis C and led to complete recovery from hepatitis in 39 of the cases.
Journal ArticleDOI
Comparison between PCR and Detection of Antigen in Sera for Diagnosis of Pulmonary Aspergillosis
Sumio Kawamura,Shigefumi Maesaki,Tetsuhiro Noda,Y. Hirakata,Kazunori Tomono,Takayoshi Tashiro,Shigeru Kohno +6 more
TL;DR: D detection of Aspergillus DNA in serum samples by nested PCR had the highest sensitivity of the three methods tested for the diagnosis of pulmonary aspergillosis.
Journal ArticleDOI
Single-molecule electrophoresis
Alonso Castro,E. Brooks Shera +1 more
TL;DR: In this paper, a novel method for the detection and identification of single molecules in solution has been devised, computer simulated, and experimentally achieved, which involves the determination of electrophoretic velocities by measuring the time required for individual molecules to travel a fixed distance between two laser beams.
Journal ArticleDOI
Hepatitis C virus infection in kidney transplant recipients
Johnson Y. N. Lau,Gary L. Davis,M. E. Brunson,Ke-Ping Qian,Hsiang-Ju Lin,Stella Quan,Robert K. Dinello,Alan Polito,Juan C. Scornik +8 more
TL;DR: The data suggest that the prevalence of hepatitis Cirus infection is high in kidney transplant recipients, active hepatitis C virus replication may be present in the absence of hepatitisC virus antibody, and many of these patients who are second‐generation enzyme immunoassay–positive but second‐ generation recombinant immunoblot assay–indeterminate are hepatitis C Virus RNA positive.
References
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Journal ArticleDOI
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
Randall Keichi Saiki,David H. Gelfand,Susanne Stoffel,Stephen J. Scharf,Russell Higuchi,Glenn Thomas Horn,Kary B. Mullis,Henry A. Erlich +7 more
TL;DR: A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified.
Book ChapterDOI
Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.
Kary B. Mullis,Fred A. Faloona +1 more
TL;DR: A method whereby a nucleic acid sequence can be exponentially amplified in vitro is described in the chapter, and the possibility of utilizing a heat-stable DNA polymerase is explored so as to avoid the need for addition of new enzyme after each cycle of thermal denaturation.
Journal ArticleDOI
DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells
Chin-Yih Ou,Shirley Kwok,Sheila W. Mitchell,David Henry Mack,John J. Sninsky,John W. Krebs,Paul M. Feorino,Donna T. Warfield,Gerald Schochetman +8 more
TL;DR: This method of DNA amplification made it possible to obtain results within 3 days, whereas virus isolation takes up to 3 to 4 weeks, and may therefore be used to complement or replace virus isolation as a routine means of determining HIV-1 infection.
Journal ArticleDOI
DNA typing from single hairs
TL;DR: Three different means of DNA typing are used for the determination of amplified DNA fragment length differences, hybridization with allele-specific oligonucleotide probes, and direct DNA sequencing on single human hairs to detect genetically variable mitochondrial and nuclear DNA sequences.
Journal ArticleDOI
Amplification and analysis of DNA sequences in single human sperm and diploid cells
Honghua Li,Ulf B. Gyllensten,Xiangfeng Cui,Randall Keichi Saiki,Henry A. Erlich,Norman Arnheim +5 more
TL;DR: The use of the polymerase chain reaction for analysing DNA sequences in individual diploid cells and human sperm shows that two genetic loci can be co-amplified from a single sperm, which may allow the analysis of previously inaccessible genetic phenomena.