Journal ArticleDOI
Avoiding false positives with PCR
S Kwok,Russell Higuchi +1 more
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TLDR
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment and adherence to a strict set of protocols can avoid disaster.Abstract:
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment. Tidiness and adherence to a strict set of protocols can avoid disaster.read more
Citations
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Journal ArticleDOI
Molecular characterization of occult hepatitis B cases in Greek blood donors
Antigoni Katsoulidou,Dimitrios Paraskevis,Emmanouil Magiorkinis,Zissis Moschidis,C. Haida,E. Hatzitheodorou,Agoritsa Varaklioti,Anastasia Karafoulidou,Maria Hatzitaki,Lilian Kavallierou,Athanasia Mouzaki,Evaggelia Andrioti,Chrysanthi Veneti,Athanasia Kaperoni,Eleftheria Zervou,Constantina Politis,Angelos Hatzakis +16 more
TL;DR: Serological and molecular characterization of occult HBV infection in Greek blood donors was undertaken and several amino acid substitutions related to diagnostic escape and antiviral resistance were revealed.
Journal ArticleDOI
Long-term follow-up of hepatitis B virus and hepatitis C virus replicative levels in chronic hepatitis patients coinfected with both viruses
Kazuyoshi Ohkawa,Norio Hayashi,Nobukazu Yuki,Manabu Masuzawa,Michio Kato,Keiji Yamamoto,Hideo Hosotsubo,Matsuo Deguchi,Kazuhiro Katayama,Akinori Kasahara,Hideyuki Fusamoto,Takenobu Kamada +11 more
TL;DR: It was found that hepatitis C virus replicative levels were enhanced after the decline of hepatitis B virus replication in 1 of the 3 patients, and that a suppressive action between hepatitis B and C viruses may occur in dual infection with both viruses.
Combination therapy with interferon alpha and ribavirin for chronic hepatitis C virus infection in thalassaemic patients
P. T. T Elfer,J. A. G Arson,K. W Hitby,P. R. G Rant,A. Y Ardumian,A. V. H Offbrand,B. W Onke +6 more
TL;DR: In this paper, a pilot study of combination anti-viral therapy for patients who failed to respond, or relapsed after an initial response to single-agent interferon-alpha was conducted.
Journal ArticleDOI
HPV DNA in plasma of patients with cervical carcinoma.
Narayanan Sathish,Priya Abraham,Abraham Peedicayil,Gopalan Sridharan,Subhashini John,Ramachandran V Shaji,George Chandy +6 more
TL;DR: HPV DNA is detectable in the plasma of patients with advanced cervical cancer and a patient with stage IB cancer had detectable HPV DNA in the external iliac lymph node, which was histopathologically free of tumour.
Journal ArticleDOI
Evaluation of indeterminate c22-3 reactivity in volunteer blood donors
Leslie H. Tobler,Michael P. Busch,J Wilber,Robert K. Dinello,Stella Quan,Alan Polito,R. Kochesky,C Bahl,M Nelles,Stephen R. Lee +9 more
TL;DR: A large number of blood donor sera that are repeatably reactive for hepatitis C virus (HCV) on second‐generation enzyme immunoassay (EIA 2.0) are indeterminate on second-generation recombinant immunoblot assay (RIBA 2.2).
References
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Journal ArticleDOI
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
Randall Keichi Saiki,David H. Gelfand,Susanne Stoffel,Stephen J. Scharf,Russell Higuchi,Glenn Thomas Horn,Kary B. Mullis,Henry A. Erlich +7 more
TL;DR: A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified.
Book ChapterDOI
Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.
Kary B. Mullis,Fred A. Faloona +1 more
TL;DR: A method whereby a nucleic acid sequence can be exponentially amplified in vitro is described in the chapter, and the possibility of utilizing a heat-stable DNA polymerase is explored so as to avoid the need for addition of new enzyme after each cycle of thermal denaturation.
Journal ArticleDOI
DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells
Chin-Yih Ou,Shirley Kwok,Sheila W. Mitchell,David Henry Mack,John J. Sninsky,John W. Krebs,Paul M. Feorino,Donna T. Warfield,Gerald Schochetman +8 more
TL;DR: This method of DNA amplification made it possible to obtain results within 3 days, whereas virus isolation takes up to 3 to 4 weeks, and may therefore be used to complement or replace virus isolation as a routine means of determining HIV-1 infection.
Journal ArticleDOI
DNA typing from single hairs
TL;DR: Three different means of DNA typing are used for the determination of amplified DNA fragment length differences, hybridization with allele-specific oligonucleotide probes, and direct DNA sequencing on single human hairs to detect genetically variable mitochondrial and nuclear DNA sequences.
Journal ArticleDOI
Amplification and analysis of DNA sequences in single human sperm and diploid cells
Honghua Li,Ulf B. Gyllensten,Xiangfeng Cui,Randall Keichi Saiki,Henry A. Erlich,Norman Arnheim +5 more
TL;DR: The use of the polymerase chain reaction for analysing DNA sequences in individual diploid cells and human sperm shows that two genetic loci can be co-amplified from a single sperm, which may allow the analysis of previously inaccessible genetic phenomena.