Journal ArticleDOI
Avoiding false positives with PCR
S Kwok,Russell Higuchi +1 more
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TLDR
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment and adherence to a strict set of protocols can avoid disaster.Abstract:
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment. Tidiness and adherence to a strict set of protocols can avoid disaster.read more
Citations
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Journal ArticleDOI
Hepatitis C Infection in Patients with Primary Hypogammaglobulinemia after Treatment with Contaminated Immune Globulin
TL;DR: In patients with primary hypogammaglobulinemia there was a high rate of HCV infection after treatment with contaminated immune globulin, and responses to interferon are poor.
Journal ArticleDOI
Increased Fetal DNA Concentrations in the Plasma of Pregnant Women Carrying Fetuses with Trisomy 21
Y.M. Dennis Lo,Tze K. Lau,Jun Zhang,Tse N. Leung,Allan M. Z. Chang,N. Magnus Hjelm,R. Sarah Elmes,Diana W. Bianchi +7 more
TL;DR: Abnormally high concentrations of circulating fetal DNA are found in a proportion of women carrying fetuses with trisomy 21, and the robustness and reproducibility of real-time PCR analysis of maternal plasma makes it a valuable tool for cross-institutional collaboration involving centers located in different parts of the world.
BookDOI
The polymerase chain reaction
TL;DR: This handbook provides up-to-date methodological protocols from the world's leading laboratories, in addition to new techniques and enhanced applications not yet available in book form.
Journal ArticleDOI
General primer-mediated polymerase chain reaction for detection of enteroviruses: application for diagnostic routine and persistent infections.
TL;DR: In this paper, the applicability of the polymerase chain reaction (PCR) for routine diagnostic use and for the detection of persistent enteroviral infections was evaluated using 66 different enterovirus serotypes and a specific fragment was amplified from 60 of 66 serotypes.
Journal ArticleDOI
Detection of Norwalk virus in stool by polymerase chain reaction.
TL;DR: A cationic detergent, cetyltrimethylammonium bromide, was found to effectively remove from stool extracts factors that inhibit the RT-PCR assay, and should allow detection of Norwalk virus in food or environmental samples such as shellfish and shellfish waters.
References
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Journal ArticleDOI
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
Randall Keichi Saiki,David H. Gelfand,Susanne Stoffel,Stephen J. Scharf,Russell Higuchi,Glenn Thomas Horn,Kary B. Mullis,Henry A. Erlich +7 more
TL;DR: A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified.
Book ChapterDOI
Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.
Kary B. Mullis,Fred A. Faloona +1 more
TL;DR: A method whereby a nucleic acid sequence can be exponentially amplified in vitro is described in the chapter, and the possibility of utilizing a heat-stable DNA polymerase is explored so as to avoid the need for addition of new enzyme after each cycle of thermal denaturation.
Journal ArticleDOI
DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells
Chin-Yih Ou,Shirley Kwok,Sheila W. Mitchell,David Henry Mack,John J. Sninsky,John W. Krebs,Paul M. Feorino,Donna T. Warfield,Gerald Schochetman +8 more
TL;DR: This method of DNA amplification made it possible to obtain results within 3 days, whereas virus isolation takes up to 3 to 4 weeks, and may therefore be used to complement or replace virus isolation as a routine means of determining HIV-1 infection.
Journal ArticleDOI
DNA typing from single hairs
TL;DR: Three different means of DNA typing are used for the determination of amplified DNA fragment length differences, hybridization with allele-specific oligonucleotide probes, and direct DNA sequencing on single human hairs to detect genetically variable mitochondrial and nuclear DNA sequences.
Journal ArticleDOI
Amplification and analysis of DNA sequences in single human sperm and diploid cells
Honghua Li,Ulf B. Gyllensten,Xiangfeng Cui,Randall Keichi Saiki,Henry A. Erlich,Norman Arnheim +5 more
TL;DR: The use of the polymerase chain reaction for analysing DNA sequences in individual diploid cells and human sperm shows that two genetic loci can be co-amplified from a single sperm, which may allow the analysis of previously inaccessible genetic phenomena.