Journal ArticleDOI
Avoiding false positives with PCR
S Kwok,Russell Higuchi +1 more
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TLDR
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment and adherence to a strict set of protocols can avoid disaster.Abstract:
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment. Tidiness and adherence to a strict set of protocols can avoid disaster.read more
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JCV-DNA and BKV-DNA in the CNS tissue and CSF of AIDS patients and normal subjects. Study of 41 cases and review of the literature
Luca Vago,Paola Cinque,Elena Sala,Manuela Nebuloni,R Caldarelli,S Racca,Pasquale Ferrante,G Trabottoni,G. Costanzi +8 more
TL;DR: It is demonstrated that JCV-DNA and, rarely, BKV-DNA can be detected in the central nervous system of immunocompromised patients with and without PML and also in the CNS of HIV-negative subjects.
Journal ArticleDOI
High frequency of leukemic clones in newborn screening blood samples of children with B-precursor acute lymphoblastic leukemia.
Jeffrey W. Taub,Mark A. Konrad,Yubin Ge,John M. Naber,Jackie S. Scott,Larry H. Matherly,Yaddanapudi Ravindranath +6 more
TL;DR: The results of this study support the notion that a high proportion of childhood B-precursor ALL cases arise in utero, although postnatal events are also important factors in leukemogenesis.
Journal ArticleDOI
Geographic distribution and genetic variability of hepatitis delta virus genotype I.
A. Obaid Shakil,A. Obaid Shakil,Stephanos J. Hadziyannis,Jay H. Hoofnagle,Adrian M. Di Bisceglie,John L. Gerin,John L. Casey +6 more
TL;DR: High sequence diversity was found for isolates from the United States, Archangelos, Turkey, and Albania, suggesting that HDV was introduced more recently and/or from fewer sources into these areas as compared to mainland Greece, Italy, and north Africa, where sequence diversity is much greater.
Journal ArticleDOI
Comparison of PCR assay with bacterial culture for detecting Streptococcus pneumoniae in middle ear fluid of children with acute otitis media.
TL;DR: P pneumolysin PCR is a sensitive and specific new method to study pneumococcal involvement in MEF samples of children with AOM by comparing a newly developed pneumitiscal PCR for Streptococcus pneumoniae to bacterial culture with 180 middle ear fluid samples of 125 children with 125 episodes of AOM.
Journal ArticleDOI
Search for Coxsackievirus B3 RNA in idiopathic dilated cardiomyopathy using gene amplification by polymerase chain reaction
Maurizia Grasso,Eloisa Arbustini,Enrico Silini,Marta Diegoli,Elena Percivalle,Giulio Ratti,M. Bramerio,Antonello Gavazzi,Mario Viganò,Gabriele Milanesi +9 more
TL;DR: A polymerase chain reaction (PCR) amplification assay was developed to detect Coxsackievirus B3 ribonucleic acid (RNA) in blood and myocardial tissue of explanted hearts from 40 patients who underwent cardiac transplantation and in 1 normal heart.
References
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Journal ArticleDOI
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
Randall Keichi Saiki,David H. Gelfand,Susanne Stoffel,Stephen J. Scharf,Russell Higuchi,Glenn Thomas Horn,Kary B. Mullis,Henry A. Erlich +7 more
TL;DR: A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified.
Book ChapterDOI
Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.
Kary B. Mullis,Fred A. Faloona +1 more
TL;DR: A method whereby a nucleic acid sequence can be exponentially amplified in vitro is described in the chapter, and the possibility of utilizing a heat-stable DNA polymerase is explored so as to avoid the need for addition of new enzyme after each cycle of thermal denaturation.
Journal ArticleDOI
DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells
Chin-Yih Ou,Shirley Kwok,Sheila W. Mitchell,David Henry Mack,John J. Sninsky,John W. Krebs,Paul M. Feorino,Donna T. Warfield,Gerald Schochetman +8 more
TL;DR: This method of DNA amplification made it possible to obtain results within 3 days, whereas virus isolation takes up to 3 to 4 weeks, and may therefore be used to complement or replace virus isolation as a routine means of determining HIV-1 infection.
Journal ArticleDOI
DNA typing from single hairs
TL;DR: Three different means of DNA typing are used for the determination of amplified DNA fragment length differences, hybridization with allele-specific oligonucleotide probes, and direct DNA sequencing on single human hairs to detect genetically variable mitochondrial and nuclear DNA sequences.
Journal ArticleDOI
Amplification and analysis of DNA sequences in single human sperm and diploid cells
Honghua Li,Ulf B. Gyllensten,Xiangfeng Cui,Randall Keichi Saiki,Henry A. Erlich,Norman Arnheim +5 more
TL;DR: The use of the polymerase chain reaction for analysing DNA sequences in individual diploid cells and human sperm shows that two genetic loci can be co-amplified from a single sperm, which may allow the analysis of previously inaccessible genetic phenomena.